Screening, Isolation and Identification of Probiotic Producing Lactobacillus acidophilus Strains EMBS081 &amp; EMBS082 by 16S rRNA Gene Sequencing

Chandok, Harshpreet; Shah, Pratik; Akare, Uday Raj; Hindala, Maliram; Bhadoriya, Sneha Singh; Ravi, G.; Sharma, Varsha; Rathore, Pragya; Nayarisseri, Anuraj

doi:10.1007/s12539-015-0002-5

Cited by 13 publications

(6 citation statements)

References 17 publications

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“…The bootstrap test (1000replicates) is shown next to the branches. Bootstrapped phenograms were produced for all the trees using consensus procedure [ 43 , 44 , 45 ]. Trees were treated as sun rooted, the 'out group designation' option was included to polarize the character states.…”

Section: Resultsmentioning

confidence: 99%

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Screening, isolation and characterization of biosurfactant producing Bacillus subtilis strain ANSKLAB03

2018

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Biosurfactants are surface-active compounds produced by a wide range of microorganisms. They have both hydrophobic and hydrophilic domains and can decrease the surface tension and the interfacial tension of growth medium. Biosurfactants have different chemical structures like-lipopeptides, glycolipids, neutral lipids and fatty acids. They are biodegradable non-toxic biomolecules that show strong emulsification of hydrophobic compounds. They have the ability to form stable emulsions. The low water-solubility of these compounds restricts their availability to microorganisms. Surfactants secreted by microbes enhance the bioavailability of such hydrophobic compounds for bioremediation. Therefore, biosurfactant-enhanced solubility of pollutants has prospective applications in bioremediation. Biosurfactants are useful in a variety of industrial processes, and are also of vital importance to the microbes in adhesion, emulsification, and bioavailability, desorption and defense strategy. Therefore, it is of interest to identify biosurfuctantproducing strain of bacteria from brackish water. The microbial samples were isolated from the Chilika Lake, odisha, India and were tested for its biosurfactant property by various biochemical methods. 16S rRNA was sequenced using Sanger dideoxy sequencing method to characterize the biosurfuctant producing strain. The new Bacillus subtilis strain ANSKLAB03 isolated from 40 samples was deposited in GenBank with accession number KU523257.

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Section: Resultsmentioning

confidence: 99%

“…Trees were treated as sun rooted, the 'out group designation' option was included to polarize the character states. A comparative study of phylogenetic trees was performed by maximum-likelihood, UPGMA methods [ 42 ] and by maximum parsimony methods [ 45 ], which resulted in similar topologies of the strain Figure 6 .…”

Section: Resultsmentioning

confidence: 99%

Screening, isolation and characterization of biosurfactant producing Bacillus subtilis strain ANSKLAB03

2018

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“…DNA extraction of Lactobacillus isolates for the molecular analysis was performed according to the method of Chandok et al (2015) with some modifications. First, pure culture of Lactobacillus isolates was prepared on MRS agar medium and 2 to 3 colonies were dissolved in 50 μl of STE (Sodium Chloride-Tris-EDTA) solution.…”

Section: Dna Extraction and Molecular Methodsmentioning

confidence: 99%

Probiotic properties of Lactobacilli isolated from human milk and their inhibitory effect on gastrointestinal pathogenic bacteria

Dallal¹,

Maragheh²,

Rajabi³

et al. 2021

Afr. J. Microbiol. Res.

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Breast feeding has critical effects on the newborns and either mother's health. Some of such healthimproving effects of the mother's milk is associated with the beneficial microbes, lactic acid bacteria (LAB), which are normally present in the mother's milk. Thus, human milk-associated lactobacilli were isolated in this study and some of their probiotic properties was investigated. Afterwards, Lactobacillus strains were screened for low pH and bile acids tolerance. Molecular identification was carried out using 16SrDNA and polymerase chain reaction (PCR). Antibiotic resistance was evaluated with disk diffusion assay and the inhibitory effect of isolates on pathogenic bacteria was examined with well assay and zone inhibition. Isolation experiments resulted in 122 human milk-associated lactobacilli belonging to 12 species. The most dominant species was Lactobacillus casei followed by Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus gasser, respectively. Screening for probiotic properties showed that 19 isolates, belonging to, Lactobacillus, have interesting probiotic characteristics. The most prevalent antibiotic resistance was observed in case of vancomycin (63.15%) and no drug resistance was detected for chloramphenicol, penicillin, rifampin (0%). Three Lactobacillus strains, designated as L4, L14 and L16, were found as potential probiotic strains since they have indicated promising inhibitory effects against the studied pathogenic bacterial strains. Our results shed light on the considerable diversity of lactobacilli in human breast milk. Furthermore, the candidate probiotic strains detected in this research might be used as potential probiotic strains.

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“…Both the forward and reverse sequence obtained from the trace file, further assembles using DNA Baser [38]. The assembled sequence obtained in FASTA format shown below was used for the further Bioinformatics analysis [39][40][41][42][43][44].…”

Section: Phylogeny Assessmentmentioning

confidence: 99%

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2018

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Cresol is an organic pollutant discharged by pharmaceutical, pesticide, coal and gasification industries causing severe organ failure in humans. Therefore, it is of interest to isolate microbes from contaminated site for degrading cresol. We isolated a strain (CR-13) that survives at 5000ppm cresol with about 80% cresol degradation ability. Immobilized cells showed >99% degradation at high concentration of cresol. The 16S rRNA sequence (accession number: MF278026) deposited in GenBank was used for phylogenetic tree analysis and the strain was grouped under Pseudomonas monteilii. The isolated cresol degrading strain was subsequently named as Pseudomonas monteilii SHY.

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Screening, Isolation and Identification of Probiotic Producing Lactobacillus acidophilus Strains EMBS081 & EMBS082 by 16S rRNA Gene Sequencing

Cited by 13 publications

References 17 publications

Screening, isolation and characterization of biosurfactant producing Bacillus subtilis strain ANSKLAB03

Screening, isolation and characterization of biosurfactant producing Bacillus subtilis strain ANSKLAB03

Probiotic properties of Lactobacilli isolated from human milk and their inhibitory effect on gastrointestinal pathogenic bacteria

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