Screening, morphological and molecular characterization of fungi producing cystathionine γ-lyase

Abstract

The potency for production of cystathionine γ-lyase (CGL) by the fungal isolates was screened. Among the tested twenty-two isolates, Aspergillus carneus was the potent CGL producer (6.29 U/mg), followed by A. ochraceous (6.03 U/mg), A. versicolor (2.51 U/mg), A. candidus (2.12 U/mg), A. niveus and Penicillium notatum (2.0 U/mg). The potent six isolates producing CGL was characterized morphologically, A. carneus KF723837 was further molecularly characterized based on the sequence of 18S-28S rDNA. Upon sulfur starvation, the yield of A. carneus extracellular CGL was increased by about 1.7-and 4.1-fold comparing to non-sulfur starved and L-methionine free medium, respectively. Also, the uptake of L-methionine was duplicated upon sulfur starvation, assuming the activation of specific transporters for L-methionine and efflux of CGL. Also, the intracellular thiols and GDH activity of A. carneus was strongly increased by S starvation, revealing the activation of in vivo metabolic antioxidant systems. upon irradiation of A. carneus by 2.0 kGy of γ-rays, the activity of CGL was increased by two-fold, regarding to control, with an obvious decreases on its yield upon further doses. Practically, CGL activity from the solid A. carneus cultures, using rice bran as substrate, was increased by 1.2-fold, comparing to submerged cultures, under optimum conditions.

Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by Penicillium chrysogenum

“…Interestingly, the results of TLC and HPLC analyzes approved taxol production by P. chrysogenum and confirmed the success of the results obtained by PCR amplification of DBAT gene as rate-limiting genes of taxol biosynthesis; the purity of P. chrysogenum taxol was additionally approved by HPLC [ 17 , 31 , 32 ]. The chemical structure of the extracted taxol from P. chrysogenum was confirmed by 1 H NMR, 13 C NMR and FTIR analysis.…”

“…Research work focused recently on discovering taxol-producing strains of endophytic fungi that are able to produce taxol as an extracellular metabolite. It was approved that these fungal isolates contain the genes encoding 10-de-acetylbaccatin III-O-acetyltransferase (DBAT), which is the precursor necessary for taxol biosynthesis [ 16 , 17 ].…”

Conjugation of Aspergillus flavipes Taxol with Porphyrin Increases the Anticancer Activity of Taxol and Ameliorates Its Cytotoxic Effects

“…The current fungal isolates were morphologically identified based on their macro and microscopical features [30]. Aspergillus flavipes was isolated from Egyptian soil samples [1,[31][32][33][34][35][36], while A. terreus, A. flavus, and A. parasiticus were isolated as endophytes from Podocarpus gracilior [6,8]. The isolates were maintained on potato dextrose agar (PDA) medium [37] (extract of 250 g potato, 20 g glucose, and 20 g agar, dissolved in 1 L tap water).…”

Biochemical and Pharmacokinetic Properties of PEGylated Cystathionine &#947;-Lyase from <b><i>Aspergillus carneus</i></b> KF723837

“…carneus KF723837 was selected as a potent CGL producer among 22 fungal isolates [El-Sayed et al, 2015]. Prior to inoculation, the fungus was grown on sulfur and nitrogen-free basal medium for 2 days at 30 ° C for starvation.…”

“…For the enormous therapeutic applications of CGL, exploration of this enzyme from fungi with a unique catalytic and biochemical properties remains a challenge. Aspergillus carneus was selected as potent isolate for producing CGL from our preliminary studies [El-Sayed et al, 2015].…”