Screening of 170 Peruvian plant species for allelopathic activity by using the Sandwich Method

Morikawa, Cecilia Inés Ono; Miyaura, Rie; Figueroa, María de Lourdes Tapia y; Salgado, Elsa Liliana Rengifo; Fujii, Yoshiharu

doi:10.1111/j.1445-6664.2011.00429.x

Cited by 21 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The sandwich bioassay has been used [33,35,44,[61][62][63] to screen a lot of plants and is effective in determining allelopathic activities by plant litter under laboratory conditions. The sandwich bioassay is widely used, reliable, and less time-consuming method for screening allelopathic activity from leaf litter [61].…”

Section: Sandwich Bioassaymentioning

confidence: 99%

Exploring Alternative Use of Medicinal Plants for Sustainable Weed Management

et al. 2017

Self Cite

View full text Add to dashboard Cite

This paper presents the first application of ethnobotanical studies to screen for allelopathic species among medicinal plants for sustainable weed management. This study assesses the possible relationship between ethnobotanical indices and allelopathy of medicinal plants. Ethnobotanical data were collected in 2016 by using semi-structured interviews with 140 informants in the Ejisu-Juaben Municipality, Ghana. Data were analysed using statistical tool and ethnobotanical indices including use value (UV), Fidelity Level (FL), Relative Frequency of Citation (RFC). The Sandwich and Dish pack methods were respectively used to evaluate allelopathy through leachates and volatiles of collected samples. Ninety-five species belonging to 43 families are reported in this study, with leaves (52%) cited the most utilised plant part. Cleistopholis patens (UV = 0.54; FL = 90.7%; RFC = 0.37) and Ocimum gratissimum (UV = 0.37; FL = 38.4%; RFC = 0.35) were among the most cited species. Thirty-two species showed inhibition (≥49.3%) by leachates, while twenty-four species were found with potential volatile inhibitory compounds against lettuce radicle growth. There was a significant positive correlation (Pearson) between the UV and RFC of medicinal plants and allelopathy by leaf leachates (r = 0.639 **; p = 0.01 and r = 0.653 **; p = 0.01 respectively). This systematic documentation of medicinal plants in Ejisu-Juaben Municipality shows medicinal plants with ethnomedicinal values and potential allelopathy that can be utilised in sustainable weed control.

show abstract

Section: Sandwich Bioassaymentioning

confidence: 99%

Exploring Alternative Use of Medicinal Plants for Sustainable Weed Management

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The sandwich method procedure was adopted from Fujii et al (2003), Fujii et al(2004), and Morikawa et al(2012). The amount of leaf litter sample (either 10 mg or 50 mg) was placed into a six well (~10 cm 2 area per well) multidish plate (36 mm × 18 mm, Nalge Nunc Int.)…”

Section: Sandwich Methodsmentioning

confidence: 99%

“…As Table 1 shows, the radicle elongation in lettuce seedlings was less than that of hypocotyls with either 10 mg or 50 mg of dried leaves. This might be related to the absorption and concentration of phytotoxins in the root tissue by direct contact with the allelochemicals (Morikawa et al, 2012).…”

Section: Allelopathic Potential Of S Macrophylla Leaf Litter On Lettmentioning

confidence: 99%

Determination of Allelopathic Potential in Mahogany (Swietenia macrophylla King) Leaf Litter Using Sandwich Method

Mukaromah

Purwestri

Fujii

2017

Indones. J. Biotechnol.

Self Cite

View full text Add to dashboard Cite

The sandwich method is a reliable screening bioassay that can be utilized to investigate allelopathic activity of leaf litter leachates. Screening the allelopathic potential of mahogany (Swietenia macrophylla King) leaf litter in plant-plant interaction using the sandwich bioassay method has not been reported. The research objectives were to determine and categorize allelopathic potential of S. macrophylla leaf litter using the sandwich bioassay method, and to determine specific activity (EC 50 ) of S. macrophylla leaf litter. The results showed that S. macrophylla leaf litter exhibited strong allelopathic activity when compared with 46 leaf litter species and was included in the top ten of allelopathic leaf litter species. Increasing S. macrophylla leaf litter concentration was concomitant with inhibition of radicle lettuce seedling growth compared with the control. According to the linear regression analysis, the effective concentration (EC 50 ) of S. macrophylla was estimated to be 3.25 mg D.W. eq. mL 1 and was considered to have strong growth inhibitory activity on lettuce radicle elongation. The results suggest the possibility of allelopathic potential of leaf litter in plant-plant interaction under S. macrophylla trees.

show abstract

“…The screening of a large number of plants by bioassay might be the first significant step in the investigation of related allelochemicals and their application in weed management. The sandwich method was developed to determine the allelopathic activity through plant leachates [2,12] and has been widely used [2,[12][13][14]. The method is reliable and less time-consuming under laboratory conditions that could be used in extensive screening in the identification of potential allelopathic species [2,12,[15][16][17].…”

Section: Introductionmentioning

confidence: 99%

First Broad Screening of Allelopathic Potential of Wild and Cultivated Plants in Turkey

Ozkan

Uruşak

Appiah

et al. 2019

Plants

Self Cite

View full text Add to dashboard Cite

Turkey has one of the richest plant diversities in the Mediterranean region. In the current literature, no broad screening has been conducted on the potential allelopathy of plants from Turkey. This study aimed to evaluate the allelopathic activity of a large number of plants from Turkey for the first time and to determine the species with significant plant growth inhibitory potentials by bioassay. Dried samples of different plant parts were collected from local herbalists. The sandwich method was used to evaluate the potential allelopathy of 126 medicinal plants belonging to 55 families. The results of lettuce radicle and hypocotyl growth for 10 and 50 mg sample treatment conformed to normal distribution. Significant inhibition on lettuce radicle elongation with 10 mg sample was observed in 40 species, out of which 27 species showed over 50% inhibitory activity. The results suggested that these species could contain potential inhibitory compounds against lettuce radicle or hypocotyl growth. The calyxes of Hibiscus sabdariffa (3.2% of control) and the seeds of Prunus dulcis (5.7% of control) showed the most potent growth inhibitory activity on lettuce radicle elongation. The potential plant growth inhibitory effects of these plants, together with the fruits of Rhus coriaria and seeds of Prunus mahaleb, have been reported in this study for the first time. All these plants are medicinal, and the results hereby presented provide essential information about the allelopathic effects of medicinal plants from Turkey.

show abstract

Screening of 170 Peruvian plant species for allelopathic activity by using the Sandwich Method

Cited by 21 publications

References 26 publications

Exploring Alternative Use of Medicinal Plants for Sustainable Weed Management

Exploring Alternative Use of Medicinal Plants for Sustainable Weed Management

Determination of Allelopathic Potential in Mahogany (Swietenia macrophylla King) Leaf Litter Using Sandwich Method

First Broad Screening of Allelopathic Potential of Wild and Cultivated Plants in Turkey

Contact Info

Product

Resources

About