Screening of 20 Mycobacterium tuberculosis sRNAs in plasma for detection of active pulmonary tuberculosis

Han, Xue; Li, Ting; Fan, Yunfan; Wang, Xinyang; Gu, Wei; Lu, Weinan; Yin, Yi-An; Meng, Qinggang; Zhang, Wenli; Zhao, Junlong; Zhang, Fengmin; Fu, Yingmei

doi:10.1016/j.tube.2021.102086

Cited by 6 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MTB-derived sRNAs MTS2823 and MTS1338 have been found to play a vital role in the stages of MTB infection [ 23 ]. These sRNAs were also reported to be highly expressed in the plasma of patients with TB [ 3 ]. Hereby the 2 cf-RNAs of bacterial origin were used as the target genes for evaluating the efficacy of the candidate reference genes.…”

Section: Resultsmentioning

confidence: 99%

“…Emerging studies have demonstrated the potential diagnostic value of cf-RNAs for TB, including host- and pathogen-derived sRNAs [ 3 , 25 , 26 ]. However, many technical and sample-related factors may impact the results of cf-RNA quantification, such as specimen type, storage condition, RNA isolation method, and the donor's physical state [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

“…Cell-free RNA (cf-RNA) molecules can be detected in the extracellular fluid, either leaked from dead cells or secreted actively by vesicles, or transported by RNA-binding proteins [ 2 ]. Cf-RNA in biofluid has recently emerged as a promising noninvasive biomarker for TB [ 3 ].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Identification of RNU44 as an Endogenous Reference Gene for Normalizing Cell-Free RNA in Tuberculosis

et al. 2022

Open Forum Infectious Diseases

Self Cite

View full text Add to dashboard Cite

Background Normalization of cell-free RNA (cf-RNA) concentration can be affected by variable experimental conditions and thus impact the performance of their diagnostic potential. Our study aimed to identify appropriate endogenous reference genes for cf-RNA biomarker evaluation in the diagnosis of tuberculosis (TB). Methods Subjects consisting of patients with TB with and without malignancy, patients with pneumonia, and healthy controls were recruited. Candidate reference genes were screened and identified by literature reviewing and RNA-Seq analysis. Expression levels of the candidate genes were determined by reverse-transcription real-time quantitative polymerase chain reaction in plasma from patients with TB and healthy controls. The stability of gene expression was assessed by geNorm, NormFinder, BestKeeper, the Comparative Delta Ct method, and RefFinder. Differential expression of 2 small RNAs (sRNAs) encoding by genome of Mycobacterium tuberculosis in plasma of patients with TB were determined by both absolute quantification and relative quantification with candidate reference genes. Results According to the stability ranking analyzed with the 5 computational programs, the top 4 candidates—miR-93, RNU44, miR-16, and glyceraldehyde 3-phosphate dehydrogenase—were used to normalize the transcript levels of 2 mycobacterial sRNAs, MTS2823 and MTS1338, which were observed to have higher copy numbers in the plasma of patients with TB. Normalization with RNU44 displayed significantly higher levels of the 2 M tuberculosis sRNAs in the patients’ plasma than those of healthy controls. Conclusions RNU44 was demonstrated as a proper endogenous gene for cf-RNA normalization in TB diagnosis.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of RNU44 as an Endogenous Reference Gene for Normalizing Cell-Free RNA in Tuberculosis

et al. 2022

Open Forum Infectious Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

“…Later work revealed that ASdes, ASpks, AS1726, and AS1890 were also present in Bacillus Calmette Guerin (BCG) mycobacterial cultures [22]. ASdes was subsequently detected in the plasma of 15 of 27 TB patients and 6 of 24 BCG vaccinated individuals [22][23][24]. Additional RNA sequencing approaches have since broadened the number of small RNAs detected in exponential phase Mtb cultures, with 19 antisense and 20 intergenic sRNAs identified [10].…”

Section: Identification and Designation Of Mycobacterial Small Rnasmentioning

confidence: 99%

Section: Identification and Designation Of Mycobacterial Small Rnasmentioning

confidence: 99%

Small RNAs Asserting Big Roles in Mycobacteria

Coskun

Płociński

Oers

2021

ncRNA

View full text Add to dashboard Cite

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb), with 10.4 million new cases per year reported in the human population. Recent studies on the Mtb transcriptome have revealed the abundance of noncoding RNAs expressed at various phases of mycobacteria growth, in culture, in infected mammalian cells, and in patients. Among these noncoding RNAs are both small RNAs (sRNAs) between 50 and 350 nts in length and smaller RNAs (sncRNA) < 50 nts. In this review, we provide an up-to-date synopsis of the identification, designation, and function of these Mtb-encoded sRNAs and sncRNAs. The methodological advances including RNA sequencing strategies, small RNA antagonists, and locked nucleic acid sequence-specific RNA probes advancing the studies on these small RNA are described. Initial insights into the regulation of the small RNA expression and putative processing enzymes required for their synthesis and function are discussed. There are many open questions remaining about the biological and pathogenic roles of these small non-coding RNAs, and potential research directions needed to define the role of these mycobacterial noncoding RNAs are summarized.

show abstract

Modulatory Impact of the sRNA Mcr11 in Two Clinical Isolates of Mycobacterium tuberculosis

Alvarez-Eraso¹,

Muñoz-Martínez²,

Alzate

et al. 2022

Curr Microbiol

View full text Add to dashboard Cite

Screening of 20 Mycobacterium tuberculosis sRNAs in plasma for detection of active pulmonary tuberculosis

Cited by 6 publications

References 36 publications

Identification of RNU44 as an Endogenous Reference Gene for Normalizing Cell-Free RNA in Tuberculosis

Identification of RNU44 as an Endogenous Reference Gene for Normalizing Cell-Free RNA in Tuberculosis

Small RNAs Asserting Big Roles in Mycobacteria

Modulatory Impact of the sRNA Mcr11 in Two Clinical Isolates of Mycobacterium tuberculosis

Contact Info

Product

Resources

About