Screening of 239 medicinal plant species for allelopathic activity using the sandwich method

Fujii, Yoshiharu; Parvez, Syeda Shahnaz; Parvez, Mohammad Masud; Ohmae, Y.; Iida, Osamu

doi:10.1046/j.1444-6162.2003.00111.x

Cited by 173 publications

(178 citation statements)

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“…While the microbioassay requires a smaller area for its execution, but is capable of evaluating a larger number of samples, the conventional bioassay with Petri plates requires more space in the germination chamber; an additional benefit regarding the sandwich-type assay, which uses 6-well plates, even larger, and spends more medium than the microbioassay (Fujii et al, 2003; This method represents an obvious alternative that optimizes resources/analyses and requires a minimum amount of donor extract in laboratories with little infrastructure, in comparison with what has been exposed by Fuji et al (2003) and Miyaura (2012). However, a standardization process to validate the efficacy of the method, with a larger number of species and types of recipient seeds, would provide reliable data on this new method.…”

Section: Resultsmentioning

confidence: 99%

PHYTOTOXIC POTENTIAL OF Ipomoea batatas EXTRACT , DETECTED THROUGH A NEW TYPE OF SANDWICH MICROBIOASSAY ON THREE SPECIES OF WEEDS

Hernández-Aro¹,

Hernández-Pérez²,

Guadalupe-Tapia

et al. 2017

Planta daninha

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Section: Resultsmentioning

confidence: 99%

PHYTOTOXIC POTENTIAL OF Ipomoea batatas EXTRACT , DETECTED THROUGH A NEW TYPE OF SANDWICH MICROBIOASSAY ON THREE SPECIES OF WEEDS

Hernández-Aro¹,

Hernández-Pérez²,

Guadalupe-Tapia

et al. 2017

Planta daninha

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“…The sandwich method procedure was adopted from Fujii et al (2003), Fujii et al(2004), and Morikawa et al(2012). The amount of leaf litter sample (either 10 mg or 50 mg) was placed into a six well (~10 cm 2 area per well) multidish plate (36 mm × 18 mm, Nalge Nunc Int.)…”

Section: Sandwich Methodsmentioning

confidence: 99%

“…However, the previous preliminary studies need extraction procedure, and suitable for a small number of samples. The sandwich method are very useful allelopathic screening for leaf leachate under laboratory conditions (Fujii et al, 2003). The sandwich method is a reliable, fast, easy bioassay and can screen a large number of samples from leaf litter leachates.…”

Section: Introductionmentioning

confidence: 99%

Determination of Allelopathic Potential in Mahogany (Swietenia macrophylla King) Leaf Litter Using Sandwich Method

Mukaromah

Purwestri

Fujii

2017

Indones. J. Biotechnol.

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The sandwich method is a reliable screening bioassay that can be utilized to investigate allelopathic activity of leaf litter leachates. Screening the allelopathic potential of mahogany (Swietenia macrophylla King) leaf litter in plant-plant interaction using the sandwich bioassay method has not been reported. The research objectives were to determine and categorize allelopathic potential of S. macrophylla leaf litter using the sandwich bioassay method, and to determine specific activity (EC 50 ) of S. macrophylla leaf litter. The results showed that S. macrophylla leaf litter exhibited strong allelopathic activity when compared with 46 leaf litter species and was included in the top ten of allelopathic leaf litter species. Increasing S. macrophylla leaf litter concentration was concomitant with inhibition of radicle lettuce seedling growth compared with the control. According to the linear regression analysis, the effective concentration (EC 50 ) of S. macrophylla was estimated to be 3.25 mg D.W. eq. mL 1 and was considered to have strong growth inhibitory activity on lettuce radicle elongation. The results suggest the possibility of allelopathic potential of leaf litter in plant-plant interaction under S. macrophylla trees.

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“…Based on the results of Fujii et al (2003) agar growth medium in the sandwich method was found to be the best method for radish seedling growth. Agar (0.5% w/v) was also applied as the growth medium.…”

Section: Preparation Of Growth Mediummentioning

confidence: 99%

“…Thus, different labora-tory screening techniques have been applied to measure allelopathy without the interference of resource competition. The sandwich method (Fujii et al 2003), agar medium selection (Fujii 1992;Wu et al 1999), the plant box method (Fujii 1994;Nishihara et al 2005) and relay seedling (Navarez and Olofsdotter 1996) have been tested for bioassays. The sandwich method is an effective tool to screen for allelopathic properties under laboratory conditions.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of allelopathic potential of safflower genotypes (Carthamus tinctorius L.)

Motamedi¹,

Karimmojeni²,

Sini³

2016

Journal of Plant Protection Research

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Forty safflower genotypes were grown under normal irrigation and drought stress. In the first experiment, the allelopathic potential of shoot residues was evaluated using the sandwich method. Each genotype residue (0.4 g) was placed in a sterile Petri dish and two layers of agar were poured on that. Radish seeds were placed on agar medium. The radish seeds were cultivated without safflower residues as the controls. The length of the radicle, hypocotyl, and fresh biomass weight and seed germination percentages were measured. A pot experiment was also done on two genotypes with the highest and two with the lowest allelopathic activity selected after screening genotypes in the first experiment. Before entering the reproductive phase, irrigation treatments (normal irrigation and drought stress) were applied. Shoots were harvested, dried, milled and mixed with the topsoil of new pots and then radish seeds were sown. The pots with safflower genotypes were used to evaluate the effect of root residue allelopathy. The shoot length, fresh biomass weight, and germination percentage were measured. Different safflower genotypes showed varied allelopathic potential. The results of the first experiment showed that Egypt and Iran-Khorasan genotypes caused maximum inhibitory responses and Australia and Iran-Kerman genotypes resulted in minimum inhibitory responses on radish seedling growth. Fresh biomass weight had the most sensitivity to safflower residues. The results of the pot experiment were consistent with the results of in vitro experiments. Residues produced under drought stress had more inhibitory effects on the measured traits. Safflower root residue may have a higher level of allelochemicals or different allelochemicals than shoot residue.

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Screening of 239 medicinal plant species for allelopathic activity using the sandwich method

Abstract: Keywords: allelopathy, leaf litter leacheate, plant species, sandwich method. time-consuming bioassay method and could be used to screen a large number of samples.

Cited by 173 publications

References 4 publications

PHYTOTOXIC POTENTIAL OF Ipomoea batatas EXTRACT , DETECTED THROUGH A NEW TYPE OF SANDWICH MICROBIOASSAY ON THREE SPECIES OF WEEDS

PHYTOTOXIC POTENTIAL OF Ipomoea batatas EXTRACT , DETECTED THROUGH A NEW TYPE OF SANDWICH MICROBIOASSAY ON THREE SPECIES OF WEEDS

Determination of Allelopathic Potential in Mahogany (Swietenia macrophylla King) Leaf Litter Using Sandwich Method

Evaluation of allelopathic potential of safflower genotypes (Carthamus tinctorius L.)

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