Screening of a &lt;i&gt;Bacillus subtilis&lt;/i&gt; Strain Producing Multiple Types of Cyclic Lipopeptides and Evaluation of Their Surface-tension-lowering Activities

Habe, Hiroshi; Taira, Toshiaki; Imura, Tomohiro

doi:10.5650/jos.ess17039

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…The virtual absence of N236 mutations in B. subtilis strains is surprising (Table 3), and could be a result of other cheater protection mechanisms at work, such as the diversification of surfactants in the subtilis clade 92,93 (though this diversity was shown to affect biofilms more than swarms 94 ). It may also suggest that NCIB 3610, the supposedly wild strain commonly used in lab studies, is likely at least partially domesticated from its original Marburg ancestor 75 based on a recent genomic analysis that found an amount of gene loss similar to that of lab strains 95 .…”

Section: Discussionmentioning

confidence: 99%

A single mutation in rapP induces cheating to prevent cheating in Bacillus subtilis by minimizing public good production

Lyons

Kolter

2018

Commun Biol

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Cooperation is beneficial to group behaviors like multicellularity, but is vulnerable to exploitation by cheaters. Here we analyze mechanisms that protect against exploitation of extracellular surfactin in swarms of Bacillus subtilis. Unexpectedly, the reference strain NCIB 3610 displays inherent resistance to surfactin-non-producing cheaters, while a different wild isolate is susceptible. We trace this interstrain difference down to a single amino acid change in the plasmid-borne regulator RapP, which is necessary and sufficient for cheater mitigation. This allele, prevalent in many Bacillus species, optimizes transcription of the surfactin operon to the minimum needed for full cooperation. When combined with a strain lacking rapP, NCIB 3610 acts as a cheater itself—except it does not harm the population at high proportions since it still produces enough surfactin. This strategy of minimal production is thus a doubly advantageous mechanism to limit exploitation of public goods, and is readily evolved from existing regulatory networks.

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Section: Discussionmentioning

confidence: 99%

A single mutation in rapP induces cheating to prevent cheating in Bacillus subtilis by minimizing public good production

Lyons

Kolter

2018

Commun Biol

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“…He et al [33] reported the presence of the LP genes sfp (surfactin), fenB (fengycin), ituD (iturin) in B. subtilis Czk1, which was obtained from the aerial roots of rubber trees. It was contemplated that the co-production of several LPs by one strain can increase their synergistic effect [34]. Iturin A and fengycin separately exhibited antifungal activity.…”

Section: Discussionmentioning

confidence: 99%

Bacillus amyloliquefaciens—Derived Lipopeptide Biosurfactants Inhibit Biofilm Formation and Expression of Biofilm-Related Genes of Staphylococcus aureus

et al. 2021

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Biosurfactants (BSs) are surface-active compounds produced by diverse microorganisms, including the genus Bacillus. These bioactive compounds possess biological activities such as antiadhesive, antimicrobial and antibiofilm effects that can lead to important applications in combating many infections. Based on these findings, we decided to investigate the antibiofilm activity of BSs from the marine Bacillus amyloliquefaciens against Staphylococcus aureus CCM 4223. Expression of biofilm-related genes was also evaluated using qRT-PCR. Isolated and partially purified BSs were identified and characterized by molecular tools and by UHPLC-DAD and MALDI-TOF/MS. Bacillus amyloliquefaciens 3/22, that exhibited surfactant activity evaluated by oil spreading assay, was characterized using the 16S rRNA sequencing method. Screening by PCR detected the presence of the sfp, srfAA, fenD and ituD genes, suggesting production of the lipopeptides (LPs) surfactin, fengycin and iturin. The above findings were further supported by the results of UHPLC-DAD and MALDI-TOF/MS. As quantified by the crystal violet method, the LPs significantly (p < 0.001) reduced biofilm formation of S. aureus in a dose-dependent manner and decreased expression of biofilm-related genes fnbA, fnbB, sortaseA and icaADBC operon. Data from our investigation indicate a promising therapeutic application for LPs isolated from B. amyloliquefaciens toward prevention of S. aureus biofilm infections.

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“…Rhodoferax sediminis JCM 32677 was evaluated as a negative control harboring no C domain sequence in the genome. In addition, Bacillus subtilis NBRC 109107 was evaluated as a positive control producing cyclic lipopeptide synthesized by NRPS [60]. The antifungal activities were tested in vitro against Botrytis cinerea MAFF 306935, Verticillium dahliae MAFF 236075, and Colletotrichum plurivorum MAFF 306007, the causal agents of fungal disease in tomatoes.…”

Section: Antifungal Activity Of Biocontrol Candidates Against Plant P...mentioning

confidence: 99%

A small step to discover candidate biological control agents from preexisting bioresources by using novel nonribosomal peptide synthetases hidden in activated sludge metagenomes

Tomita,

Kuroda,

Narihiro

2023

PLoS ONE

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Biological control agents (BCAs), beneficial organisms that reduce the incidence or severity of plant disease, have been expected to be alternatives to replace chemical pesticides worldwide. To date, BCAs have been screened by culture-dependent methods from various environments. However, previously unknown BCA candidates may be buried and overlooked because this approach preferentially selects only easy-to-culture microbial lineages. To overcome this limitation, as a small-scale test case, we attempted to explore novel BCA candidates by employing the shotgun metagenomic information of the activated sludge (AS) microbiome, which is thought to contain unutilized biological resources. We first performed genome-resolved metagenomics for AS taken from a municipal sewage treatment plant and obtained 97 nonribosomal peptide synthetase (NRPS)/polyketide synthase (PKS)-related gene sequences from 43 metagenomic assembled bins, most of which were assigned to the phyla Proteobacteria and Myxococcota. Furthermore, these NRPS/PKS-related genes are predicted to be novel because they were genetically dissimilar to known NRPS/PKS gene clusters. Of these, the condensation domain of the syringomycin-related NRPS gene cluster was detected in Rhodoferax- and Rhodocyclaceae-related bins, and its homolog was found in previously reported AS metagenomes as well as the genomes of three strains available from the microbial culture collections, implying their potential BCA ability. Then, we tested the antimicrobial activity of these strains against phytopathogenic fungi to investigate the potential ability of BCA by in vitro cultivation and successfully confirmed the actual antifungal activity of three strains harboring a possibly novel NRPS gene cluster. Our findings provide a possible strategy for discovering novel BCAs buried in the environment using genome-resolved metagenomics.

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Screening of a <i>Bacillus subtilis</i> Strain Producing Multiple Types of Cyclic Lipopeptides and Evaluation of Their Surface-tension-lowering Activities

Cited by 5 publications

References 32 publications

A single mutation in rapP induces cheating to prevent cheating in Bacillus subtilis by minimizing public good production

A single mutation in rapP induces cheating to prevent cheating in Bacillus subtilis by minimizing public good production

Bacillus amyloliquefaciens—Derived Lipopeptide Biosurfactants Inhibit Biofilm Formation and Expression of Biofilm-Related Genes of Staphylococcus aureus

A small step to discover candidate biological control agents from preexisting bioresources by using novel nonribosomal peptide synthetases hidden in activated sludge metagenomes

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