Screening of Additive Manufactured Scaffolds Designs for Triple Negative Breast Cancer 3D Cell Culture and Stem-Like Expansion

Polonio‐Alcalá, Emma; Rabionet, Marc; Guerra, Antonio J.; Yeste, Marc; Ciurana, Joaquim; Puig, Teresa

doi:10.3390/ijms19103148

Cited by 28 publications

(18 citation statements)

References 49 publications

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“…[60][61][62][63][64][65] Commonly used materials for constructing scaffolds are bioactive ceramics (hydroxyapatite, bioactive glasses), synthetic polymers (such as polyglycolic and polylactic acids), and natural polymers (including silk, collagen, chitosan-alginate (CA), and hyaluronic acid (HA)). [66][67][68][69] Polonio-Alcalá et al 70 found that polylactic acid (PLA) scaffolds can promote CSC proliferation and enrichment in breast cancer cells. Florczyk and coworkers 71 showed that 3D CA scaffolds promoted enrichment of the CSC population, including the cells of prostate carcinoma, breast cancer, hepatocellular carcinoma (HCC), and glioblastoma cells.…”

Section: Biomaterial-based 3d Csc Culture Modelsmentioning

confidence: 99%

3D culture technologies of cancer stem cells: promising ex vivo tumor models

et al. 2020

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Cancer stem cells have been shown to be important in tumorigenesis processes, such as tumor growth, metastasis, and recurrence. As such, many three-dimensional models have been developed to establish an ex vivo microenvironment that cancer stem cells experience under in vivo conditions. Cancer stem cells propagating in three-dimensional culture systems show physiologically related signaling pathway profiles, gene expression, cell–matrix and cell–cell interactions, and drug resistance that reflect at least some of the tumor properties seen in vivo. Herein, we discussed the presently available Cancer stem cell three-dimensional culture models that use biomaterials and engineering tools and the biological implications of these models compared to the conventional ones.

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Section: Biomaterial-based 3d Csc Culture Modelsmentioning

confidence: 99%

3D culture technologies of cancer stem cells: promising ex vivo tumor models

et al. 2020

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“…Moreover, this technology could offer several significant advances: • Creation of ideal products. Polymers are common in different medical applications such as tissue engineering [2], and the manufacture of surgical planning prototypes [3][4][5][6][7] and scaffolds [8]. Ceramic materials have also been employed in scaffolds [9].…”

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confidence: 99%

Development of AM Technologies for Metals in the Sector of Medical Implants

Buj-Corral

Tejo-Otero

Fenollosa-Artés

2020

Metals

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Additive manufacturing (AM) processes have undergone significant progress in recent years, having been implemented in sectors as diverse as automotive, aerospace, electrical component manufacturing, etc. In the medical sector, different devices are printed, such as implants, surgical guides, scaffolds, tissue engineering, etc. Although nowadays some implants are made of plastics or ceramics, metals have been traditionally employed in their manufacture. However, metallic implants obtained by traditional methods such as machining have the drawbacks that they are manufactured in standard sizes, and that it is difficult to obtain porous structures that favor fixation of the prostheses by means of osseointegration. The present paper presents an overview of the use of AM technologies to manufacture metallic implants. First, the different technologies used for metals are presented, focusing on the main advantages and drawbacks of each one of them. Considered technologies are binder jetting (BJ), selective laser melting (SLM), electron beam melting (EBM), direct energy deposition (DED), and material extrusion by fused filament fabrication (FFF) with metal filled polymers. Then, different metals used in the medical sector are listed, and their properties are summarized, with the focus on Ti and CoCr alloys. They are divided into two groups, namely ferrous and non-ferrous alloys. Finally, the state-of-art about the manufacture of metallic implants with AM technologies is summarized. The present paper will help to explain the latest progress in the application of AM processes to the manufacture of implants.

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“…Previously, our research group set up the conditions of the ES-scaffolds using polycaprolactone (PCL) to enrich the BCSC population in MDA-MB-231 cells [26,32]. BCSCs are a small subpopulation of cells capable of self-renewal, giving rise to the bulk of the tumour and responsible for relapse due to their chemoresistance [33,34,35].…”

Section: Resultsmentioning

confidence: 99%

PLA Electrospun Scaffolds for Three-Dimensional Triple-Negative Breast Cancer Cell Culture

et al. 2019

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Three-dimensional (3D) systems provide a suitable environment for cells cultured in vitro since they reproduce the physiological conditions that traditional cell culture supports lack. Electrospinning is a cost-effective technology useful to manufacture scaffolds with nanofibers that resemble the extracellular matrix that surround cells in the organism. Poly(lactic acid) (PLA) is a synthetic polymer suitable for biomedical applications. The main objective of this study is to evaluate electrospun (ES)-PLA scaffolds to be used for culturing cancer cells. Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype with no validated targeted therapy and a high relapse rate. MDA-MB-231 TNBC cells were grown in scaffolds from two different PLA concentrations (12% and 15% w/v). The appropriateness of ES-PLA scaffolds was evaluated using a cell proliferation assay. EGFR and STAT3 gene expression and protein levels were compared in cells grown in 2D versus in 3D cultures. An increase in STAT3 activation was shown, which is related to self-renewal of cancer stem cells (CSCs). Therefore, the enrichment of the breast CSC (BCSC) population was tested using a mammosphere-forming assay and gene expression of BCSC-related stemness and epithelial-to-mesenchymal transition markers. Based on the results obtained, ES-PLA scaffolds are useful for 3D cultures in short culture periods with no BCSC-enrichment.

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Screening of Additive Manufactured Scaffolds Designs for Triple Negative Breast Cancer 3D Cell Culture and Stem-Like Expansion

Cited by 28 publications

References 49 publications

3D culture technologies of cancer stem cells: promising ex vivo tumor models

3D culture technologies of cancer stem cells: promising ex vivo tumor models

Development of AM Technologies for Metals in the Sector of Medical Implants

PLA Electrospun Scaffolds for Three-Dimensional Triple-Negative Breast Cancer Cell Culture

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