Screening of anti-functional dyspepsia compounds in Cynanchum auriculatum: A spectrum-effect relationship analysis, and ATP-binding cassette transporters inhibitor evaluation

Sun, Jia; Gou, Jian; Lan, Qing; Liu, Ting; Huang, Yong; Lu, Yuan; Wang, Yonglin; Li, Yongjun

doi:10.1016/j.jep.2023.116867

Cited by 5 publications

(12 citation statements)

References 22 publications

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“…Furthermore, for the YMC-Triart column (Figure S3C), isoliquiritigenin and glycyrrhizin were detected at the same retention time, and the resolution of poncirin was not as good as that achieved with the other columns. Although the resolution of 4-hydroxycinnamic acid on the SunFire TM column was slightly low, we were 5), ononin (6), baicalin (7), poncirin (8), wogonoside (9), baicalein (10), isoliquiritigenin (11), glycyrrhizin (12), and wogonin (13). The concentrations of each marker compound in the mixed standard solution were as follows: 4-hydroxycinnamic acid (10.00 µg/mL); ononin, baicalin, wogonoside, baicalein, isoliquiritigenin, and wogonin (20.00 µg/mL); narirutin and naringin (30.00 µg/mL); liquiritin (40.00 µg/mL); liquiritin apioside and poncirin (50.00 µg/mL); and glycyrrhizin (100.00 µg/mL).…”

Section: Optimization Of Hplc Conditions For Development Of the Simul...mentioning

confidence: 83%

“…Wavelength for quantification, linear range, regression equation, coefficient of determination (r 2 ), limit of detection (LOD), and limit of quantitation (LOQ) using HPLC-PDA simultaneous analysis of the 13 marker compounds in YWS. 5), ononin (6), baicalin (7), poncirin (8), wogonoside (9), baicalein (10), isoliquiritigenin (11), glycyrrhizin (12), and wogonin (13). 2 y: peak area of compounds; x: concentration (µg/mL) of compounds.…”

Section: Methods Validation Of the Developed Hplc Analysis Assaymentioning

confidence: 99%

“…, baicalein (10), isoliquiritigenin (11), glycyrrhizin (12), and wogonin (13). The concentrations of the 13 marker compounds in the mixed standard solution were as follows: 12 µg/L (isoliquiritigenin), 280 µg/L (liquiritin and ononin), 300 µg/L (4-hydroxycinnamic acid), 520 µg/L (wogonin), 1040 µg/L (narirutin), 1080 µg/L (poncirin), 1400 µg/L (baicalein), 2320 µg/L (isoliquiritin apioside), 4800 µg/L (naringin), 7600 µg/L (wogonoside), 8000 µg/L (glycyrrhizin), and 28,000 µg/L (baicalin).…”

Section: Analytementioning

confidence: 99%

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Simultaneous Analysis of Thirteen Compounds in Yeokwisan Using High-Performance Liquid Chromatography–Photodiode Array Detection and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry and Their Antioxidant Effects

Seo,

Kim,

Kim

2024

Pharmaceuticals

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Yeokwisan (YWS) is an herbal medicine prescription consisting of six oriental herbal medicines, developed to treat reflux esophagitis. We focused on developing an analytical method capable of simultaneously quantifying 13 compounds in YWS samples using high-performance liquid chromatography–photodiode array detection (HPLC–PDA) and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) and exploring their antioxidant effects. All compounds examined in both analytical systems were chromatographically separated on a SunFireTM C18 (4.6 × 250 mm, 5 μm) column and an Acquity UPLC BEH C18 (2.1 × 100 mm, 1.7 μm) column using gradient elution of a water–acetonitrile mobile phase. Antioxidant effects were evaluated based on radical scavenging activity (DPPH and ABTS tests) and ferrous ion chelating activity. In two analytical methods, the coefficient of determination of the regression equation was ≥0.9965, the recovery range was 81.11–108.21% (relative standard deviation (RSD) ≤ 9.33%), and the precision was RSD ≤ 11.10%. Application of the optimized analysis conditions gave quantitative analysis results for YWS samples of 0.02–100.36 mg/g. Evaluation of the antioxidant effects revealed that baicalein and baicalin exhibit significant antioxidant activity, suggesting that they play an important role in the antioxidant effects of YWS.

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Section: Optimization Of Hplc Conditions For Development Of the Simul...mentioning

confidence: 83%

Section: Methods Validation Of the Developed Hplc Analysis Assaymentioning

confidence: 99%

Section: Analytementioning

confidence: 99%

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Simultaneous Analysis of Thirteen Compounds in Yeokwisan Using High-Performance Liquid Chromatography–Photodiode Array Detection and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry and Their Antioxidant Effects

Seo,

Kim,

Kim

2024

Pharmaceuticals

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“…Sun et al preliminarily confirmed that scopoletin isolated from Cynachum auriculatum has an anti-functional dyspepsia effect (Sun et al, 2024). Scopoletin remarkably prevents acid reflux esophagitis production, with a similar efficiency to that of standard anti-secretory agents (ranitidine and lansoprazole) through its anti-inflammatory and anti-secretory attributes, such as its pro-kinetic activity, which can accelerate gastric emptying and intestinal transit (Mahattanadul et al, 2011).…”

Section: Facilitating the Digestion Activitymentioning

confidence: 99%

Scopoletin: a review of its pharmacology, pharmacokinetics, and toxicity

Gao,

Li,

Zhang

et al. 2024

Front. Pharmacol.

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Scopoletin is a coumarin synthesized by diverse medicinal and edible plants, which plays a vital role as a therapeutic and chemopreventive agent in the treatment of a variety of diseases. In this review, an overview of the pharmacology, pharmacokinetics, and toxicity of scopoletin is provided. In addition, the prospects and outlook for future studies are appraised. Scopoletin is indicated to have antimicrobial, anticancer, anti-inflammation, anti-angiogenesis, anti-oxidation, antidiabetic, antihypertensive, hepatoprotective, and neuroprotective properties and immunomodulatory effects in both in vitro and in vivo experimental trials. In addition, it is an inhibitor of various enzymes, including choline acetyltransferase, acetylcholinesterase, and monoamine oxidase. Pharmacokinetic studies have demonstrated the low bioavailability, rapid absorption, and extensive metabolism of scopoletin. These properties may be associated with its poor solubility in aqueous media. In addition, toxicity research indicates the non-toxicity of scopoletin to most cell types tested to date, suggesting that scopoletin will neither induce treatment-associated mortality nor abnormal performance with the test dose. Considering its favorable pharmacological activities, scopoletin has the potential to act as a drug candidate in the treatment of cancer, liver disease, diabetes, neurodegenerative disease, and mental disorders. In view of its merits and limitations, scopoletin is a suitable lead compound for the development of new, efficient, and low-toxicity derivatives. Additional studies are needed to explore its molecular mechanisms and targets, verify its toxicity, and promote its oral bioavailability.

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“…Previous laboratory studies have found that the 60% ethanol–eluted fraction from the CA extract is the main active fraction promoting gastrointestinal motility ( Liu et al, 2018 ). The possible anti–FD components of CA were screened, such as syringic acid, deacylmetaplexigenin, ferulic acid, scopoletin, baishouwu benzophenone, and qingyangshengenin by combining spectrum–effect relationship modeling ( Sun et al, 2023 ). Oral absorption studies of CA extract revealed that the overall absorption of mainly anti-FD components are lower in FD state than in the normal state ( Liu et al, 2022 ; Sun et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and tissue distribution of four major bioactive components of Cynanchum auriculatum extract: a UPLC–MS/MS study in normal and functional dyspepsia rats

Sun,

Meng,

Huang

et al. 2023

Front. Pharmacol.

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Introduction:Cynanchum auriculatum (CA) is usually used to treat digestive disorders, such as anorexia, enteritis, dysentery, and indigestion. Functional dyspepsia (FD) is characterized by a group of symptoms associated with the gastroduodenal region. Recent pharmacological studies have demonstrated the efficacy of CA for treating FD. However, the pharmacokinetics (PK) and tissue distribution of CA in physiological and FD states is still unclear. The present study aimed to clarify the differences in PK parameters and tissue distribution of the four major active components of CA (baishouwu benzophenone, deacylmet-aplexigenin, qingyangshengenin, and syringic acid) under both physiological and FD states.Methods: For this, normal and FD rats were orally administered 10 mg/kg CA extract. Then, plasma and tissue (heart, liver, spleen, lung, kidney, brain, stomach, and small intestine) samples were obtained. The four active components of CA in rat plasma and tissues were quantified by developing and validating a fast and reliable ultra–high–performance liquid chromatography–mass spectrometry method.Results: The area under the plasma concentration–time curve from time zero to time t (AUC0-t) of baishouwu benzophenone was significantly lower in the FD group than in the normal group (p < 0.01). The FD group had significantly lower (p < 0.001) apparent volume of distribution and plasma clearance of qing-yangshengenin and significantly higher (p < 0.05) AUC0-t of deacylmetaplexigenin and qingyangshengenin. The four active components were rapidly distributed into various tissues, and the main target organs of CA activity were the stomach and small intestine. In addition, baishouwu benzophenone, deacylmetaplexigenin, and qingyangshengenin could cross the blood-brain barrier, indicating that the brain may be another target organ in the treatment of FD.Discussion: These results indicate that the pathological state of FD alters the PK behavior and tissue distribution characteristics of baishouwu benzophenone, deacylmetaplexigenin, qingyangshengenin, and syringic acid in the CA extract, providing an experimental basis for the role of CA in FD treatment.

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Screening of anti-functional dyspepsia compounds in Cynanchum auriculatum: A spectrum-effect relationship analysis, and ATP-binding cassette transporters inhibitor evaluation

Cited by 5 publications

References 22 publications

Simultaneous Analysis of Thirteen Compounds in Yeokwisan Using High-Performance Liquid Chromatography–Photodiode Array Detection and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry and Their Antioxidant Effects

Simultaneous Analysis of Thirteen Compounds in Yeokwisan Using High-Performance Liquid Chromatography–Photodiode Array Detection and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry and Their Antioxidant Effects

Scopoletin: a review of its pharmacology, pharmacokinetics, and toxicity

Pharmacokinetics and tissue distribution of four major bioactive components of Cynanchum auriculatum extract: a UPLC–MS/MS study in normal and functional dyspepsia rats

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