Screening of antiangiogenic potential of twenty two marine invertebrate extracts of phylum Mollusca from South East Coast of India

Gupta, Pankaj; Muthuvel, A.; Azad, Raj Vardhan; Saxena, Rohit; Ghose, Supriyo; Biswas, Nihar Ranjan; Velpandian, Thirumurthy

doi:10.12980/apjtb.4.2014c701

Cited by 13 publications

(6 citation statements)

References 27 publications

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“…In a recent study, Bojnourdi et al [ 20 ] investigated the anti-angiogenic properties of shell extract of the mollusc chiton on CAM model and found that it produced maximum 58% anti-angiogenic activity. In another interesting study by Gupta et al [ 21 ], 22 different marine invertebrate extracts were screened for their anti-angiogenic activity, out of which, Meretrix meretrix (47.01% inhibition), Meretrix casta (64.63% inhibition), Telescopium telescopium (62.02% inhibition) and Bursa crumena (60.48% inhibition) showed significant anti-angiogenic activity in CAM. In the present study, EAME showed 42% inhibition of branching points and 51% inhibition of first order blood vessels in CAM (Fig.…”

Section: Discussionmentioning

confidence: 99%

Methanolic extract of Euchelus asper exhibits in-ovo anti-angiogenic and in vitro anti-proliferative activities

Agrawal¹,

Chaugule²,

More³

et al. 2017

Biol Res

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BackgroundThe marine environment is a rich source of bioactive natural products. Many of the marine bioactive compounds have been derived successfully from molluscs. Euchelus asper is a marine mollusc which is commonly found in the intertidal rocky regions of the Mumbai coast. The present study was focused on evaluating the anti-angiogenic and anti- proliferative activities of methanolic extract of Euchelus asper (EAME).MethodsThe anti-angiogenic activity of EAME (50–800 μg/mL) was assessed by chick chorio-allantoic membrane (CAM) model wherein multiple parameters in the CAM blood vessels were analysed through morphometric and histological investigations. In vitro testing of EAME (5–20 μg/mL) included its cytotoxicity against three different cancer cell lines, its effect on cell proliferation by wound healing assay as well as their relevant molecular mechanisms. Statistical analysis was carried out by two-tailed student’s t test for two unpaired groups.ResultsAnalysis of CAM revealed that the extract is effective in reducing the branching points of the 1st order blood vessels or capillaries of CAM. Histological analysis of CAM showed significant decrease in capillary plexus and compartmentalization along with increase in mesodermal blood vessels, thus establishing its anti-angiogenicity. Further, EAME exhibited moderate but significant cytotoxicity against A549 non-small cell lung carcinoma cell line. We also demonstrated that the cytotoxicity of EAME in A549 was associated with its apoptotic activity by subG1 phase arrest. Lastly, EAME significantly reduced A549 proliferation by reducing the expression of Matrix metalloproteinase-2 (MMP-2) and Matrix metalloproteinase-9 (MMP-9).ConclusionOverall, our study suggested that EAME has potential to inhibit tumour angiogenic and proliferative activity and may be a potential source for development of new anti-cancer pharmaceuticals.

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Section: Discussionmentioning

confidence: 99%

Methanolic extract of Euchelus asper exhibits in-ovo anti-angiogenic and in vitro anti-proliferative activities

Agrawal¹,

Chaugule²,

More³

et al. 2017

Biol Res

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“…On the 14th day, percentage inhibition of neovascularization was seen after enucleating the animals. Rats were anesthetized and sacrificed; the eyes were removed to check for inhibitory effect [12].…”

Section: Corneal Neovascularization Methodsmentioning

confidence: 99%

Anti-angiogenic activity of chloride and potassium channel modulators: repurposing ion channel modulators

et al. 2020

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Background: Excessive angiogenesis can be the root cause of many pathological conditions. Various types of ion channels are found on the endothelial cells. These ion channels play a vital role in the multi-stepped process of angiogenesis. The study aims to investigate the anti-angiogenic effects of specific ion channel modulators mefloquine (volume-regulated chloride channel blocker), lubiprostone (ClC-2 channel agonist), and 4-aminopyridine (voltage-gated potassium channel blocker). Results: The anti-angiogenic activity of ion channel modulators was screened by measuring its effects on the area of neovascularization and histopathological studies by in vivo (corneal neovascularization) method and by in vitro assays, endothelial cell proliferation assay, cell migration assay, and matrigel cord-like morphogenesis assay. The test and standard drug (bevacizumab) groups were compared with the control group using one-way ANOVA, followed by post hoc test, and Dunnett's test to compare the mean of all the groups with the control mean. The results revealed that mefloquine at the dose of 0.6% w/v and 1.0% w/v, lubiprostone at the dose of 0.5% w/v and 1.0% w/ v, and 4-aminopyridine at the dose of 2% w/v and 4% w/v showed significant anti-angiogenic property. In the studies on human umbilical vein endothelial cells, the test drugs (100 nM) showed significant inhibition of proliferation, migration, and decrease in network length of cord-like tubes. Conclusion: The scientific findings indicate that the test drugs have potent anti-angiogenic activity by inhibiting the cell proliferation, inhibiting the cell volume increase, arresting the cell cycle progression and by causing membrane hyperpolarization. The potent anti-angiogenic drugs obtained by repurposing these ion channel modulators, in the further studies, will be able to treat the diseases due to excess angiogenesis from the root cause.

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“…U. reticulata presented the maximum contents of chromium, copper and magnesium. At the same time, Halimeda tuna only exhibited low mineral contents of cobalt, iron, magnesium, manganese, nickel, lead and zinc (Gokulkrishnan et al, 2011). Besides, C. lentillifera from the Kei Islands of Indonesia contained magnesium, potassium and zinc, whereas C. lentillifera from the Seribu Islands contained calcium (119.20 g/kg), sodium (34.18 g/kg) and iron (0.34 g/kg), respectively (Tapotubun et al, 2020).…”

Section: Mineralsmentioning

confidence: 97%

“…Seafood, including seaweed species, are usually known to hold an abundance of minerals such as iodine, magnesium, calcium, phosphorus, iron, potassium, copper and fluoride (Gokulkrishnan, Anantharaman, Manivannan, Thirumaran, & Balasubramanian, 2011). These minerals play a significant role as a cofactor of the enzyme in the human body.…”

Section: Mineralsmentioning

confidence: 99%

“…These minerals play a significant role as a cofactor of the enzyme in the human body. For instance, calcium and magnesium help maintain the bone and teeth strength, while sodium and potassium involve in the transfer of nutrients (Gokulkrishnan et al, 2011). Hence, it is believed that seaweeds are valuable for human consumption, as studies showed that seaweeds have great minerals content.…”

Section: Mineralsmentioning

confidence: 99%

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Bioactive Compounds, Cosmeceutical And Nutraceutical Applications of Green Seaweed Species (Chlorophyta)

Taher¹,

Ruslan

Susanti

et al. 2021

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Seaweeds are valuable marine plants that have garnered much attention from the public due to their high bioactive, nutrients and minerals content. Seaweeds have been used in multiple applications, including in cosmeceutical, nutraceutical and pharmaceutical industries. Nevertheless, this review will focus on the bioactive compounds of Chlorophyta and their potential application in nutraceutical and cosmeceutical industries. Chlorophyta are believed to possess a significant amount of nutrients and minerals, sufficient to meet the daily requirements of nutrients and minerals in the human body. Considering the nutritional aspect, deficiency in nutrients may lead to severe ailments, including heart disease, neurological disorder and cancer. The main compounds studied in this review are polysaccharides, proteins, amino acids, lipids, fatty acids, pigments, minerals, vitamins and secondary metabolites. Among all, polysaccharides are the most exploited compounds and used in many advanced applications in the nutraceutical and cosmeceutical industries. This review also offers insights into the beneficial biological properties of Chlorophyta, highlighting their potential in cosmeceutical and nutraceutical applications. Further research is required to highlight the Chlorophyta sp. aquaculture, its extraction method, and the most targeted bioactive compounds from the species. Therefore, the challenge is to increase public awareness of the promising application of this species in the nutraceutical and cosmeceutical industries.

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Screening of antiangiogenic potential of twenty two marine invertebrate extracts of phylum Mollusca from South East Coast of India

Abstract: The significant antiangiogenic activity evinced by the extract of Telescopium telescopium merits further investigation for ocular neovascular diseases.

Cited by 13 publications

References 27 publications

Methanolic extract of Euchelus asper exhibits in-ovo anti-angiogenic and in vitro anti-proliferative activities

Methanolic extract of Euchelus asper exhibits in-ovo anti-angiogenic and in vitro anti-proliferative activities

Anti-angiogenic activity of chloride and potassium channel modulators: repurposing ion channel modulators

Bioactive Compounds, Cosmeceutical And Nutraceutical Applications of Green Seaweed Species (Chlorophyta)

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