Screening of Antibacterial and Antioxidant Activity of Biogenically Synthesized Silver Nanoparticles from Alternaria alternata, Endophytic Fungus of Dendrophthoe falcata-a Parasitic Plant

Govindappa, M.; Dj, Manasa; Vinaykiya, Vridhi; Bhoomika, V; Dutta, Suryanshi; Pawar, Ritu; Raghavendra, Vinay B.

doi:10.1007/s12668-021-00932-4

Cited by 15 publications

(2 citation statements)

References 39 publications

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“…The observation was made that Ag-NPs tended to aggregate into small clusters, ultimately resulting in the formation of bigger particles. The outcome of this experiment yielded Ag-NPs that were consistent with prior research findings ( Nirmala and Sridevi, 2021 ; M et al, 2022 ; Saied E. et al, 2023 ). Due to their excellent electrical conductivity, metal nanoparticles such as gold and silver can be easily examined using a SEM.…”

Section: Resultssupporting

confidence: 85%

Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities

Saied,

Abdel-Maksoud,

Alfuraydi

et al. 2024

Front. Microbiol.

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In the current study, endophytic Aspergillus hiratsukae was used for the biosynthesis of silver nanoparticles (Ag-NPs) for the first time. The characterizations were performed using X ray diffraction (XRD), Transmission electron microscopy (TEM), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX), Dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and UV–Vis spectroscopy. The obtained results demonstrated the successful formation of crystalline, spherical Ag-NPs with particle diameters ranging from 16 to 31 nm. The FT-IR studied and displayed the various functional groups involved, which played a role in capping and reducing agents for Ag-NPs production. The SEM–EDX revealed that the main constituent of the AS-formed sample was primarily Ag, with a weight percentage of 64.2%. The mycosynthesized Ag-NPs were assessed for antimicrobial as well as photocatalytic activities. The antimicrobial results indicated that the synthesized Ag-NPs possess notable antibacterial efficacy against Staphylococcus aureus, Bacillus subtilis, and Escherichia coli, with minimum inhibitory concentrations (MICs) of Ag-NPs ranging from 62.5 to 250 μg/mL. Moreover, the biosynthesized Ag-NPs demonstrated weak antifungal activity against Aspergillus brasiliensis and Candida albicans, with MICs of 500 and 1,000 μg/mL, respectively. In addition, the mycosynthesized Ag-NPs exhibited photocatalytic activity toward acid black 2 (nigrosine) dye under both light and dark stimulation. Notably, After 300 min exposure to light, the nigrosine dye was degraded by 93%. In contrast, 51% degradation was observed after 300 min in darkness. In conclusion, Ag-NPs were successfully biosynthesized using endophytic A. hiratsukae and also exhibited antimicrobial and photocatalytic activities that can be used in environmental applications.

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Section: Resultssupporting

confidence: 85%

Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities

Saied,

Abdel-Maksoud,

Alfuraydi

et al. 2024

Front. Microbiol.

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“…As with today's emphasis on technological innovation, the research and development of new antimicrobial agents that can enhance antibacterial activity is becoming more important in the human living environment [2][3][4], and increased bacterial resistance and cross-infection in public places are also common health threats [5][6][7]. Silver nanoparticles (Ag NPs), which are used as a common antibacterial material, have been extensively studied in the past several decades [8,9], for example, in inhibiting the growth of bacteria [10], antimicrobial activity endurance [11], and the lack of risk of resistance of bacteria [12]. Relevant experiments have demonstrated the antibacterial mechanism of silver, that is, silver ions can cause the denaturation of proteins in the cell membrane of bacteria [13][14][15], and when the Ag + enters into the bacterial cell, it quickly combines with DNA in the cell and prevents the replication of the DNA double-helix structure [16].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of CuO and PAA-Regulated Silver-Carried CuO Nanosheet Composites and Their Antibacterial Properties

Wan

Tang

et al. 2022

Polymers

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With the aid of a facile and green aqueous solution approach, a variety of copper oxide (CuO) with different shapes and polyacrylic-acid (PAA)-regulated silver-carried CuO (CuO@Ag) nanosheet composites have been successfully produced. The point of this article was to propose a common synergy using Ag-carried CuO nanosheet composites for their potential antibacterial efficiency against three types of bacteria such as E. coli, P. aeruginosa, and S. aureus. By using various technical means such as XRD, SEM, and TEM, the morphology and composition of CuO and CuO@Ag were characterized. It was shown that both CuO and CuO@Ag have a laminar structure and exhibit good crystallization, and that the copper source and reaction duration have a sizable impact on the morphology and size distribution of the product. In the process of synthesizing CuO@Ag, the appropriate amount of polyacrylic acid (PAA) can inhibit the agglomeration of Ag NPs and regulate the size of Ag at about ten nanometers. In addition, broth dilution, optical density (OD 600), and electron microscopy analysis were used to assess the antimicrobial activity of CuO@Ag against the above three types of bacteria. CuO@Ag exhibits excellent synergistic and antibacterial action, particularly against S. aureus. The antimicrobial mechanism of the CuO@Ag nanosheet composites can be attributed to the destruction of the bacterial cell membrane and the consequent leakage of the cytoplasm by the release of Ag+ and Cu2+. The breakdown of the bacterial cell membrane and subsequent leakage of cytoplasm caused by Ag+ and Cu2+ released from antimicrobial agents may be the cause of the CuO@Ag nanosheet composites’ antibacterial action. This study shows that CuO@Ag nanosheet composites have good antibacterial properties, which also provides the basis and ideas for the application research of other silver nanocomposites.

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Antioxidant Activity and Phytopathogenic Control of Extracts and Fraction from Struthanthus calophyllus A.C.Sm. (Loranthaceae)

Vanegas

Suaza-Gaviria

Ocampo-Jiménez

et al. 2023

Chemistry & Biodiversity

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Phytopathogenic microorganisms cause oxidative stress in host plants, thus affecting agricultural crops. Such stress could be controlled by antioxidant compounds from parasitic plants, given their antioxidant power. This article reports an evaluation of Struthanthus calophyllus antimicrobial activity and antioxidant mechanism by testing different polarity extracts. Antimicrobial activity was evaluated against phytopathogens bacteria (e. g., Erwinia, Pectobacterium, Xanthomonas) using the agar diffusion method. Pectobacterium and Xanthomonas presented growth inhibition zones similar to streptomycin control. Antioxidant activity was determined by measuring total phenol content, DPPH⋅, and ABTS⋅+ radicals‐scavenging inhibition percentage (IP). Most polar extracts presented 76,9–95,9 % IP, which correlates with its phenolic content. Besides, Nuclear Magnetic Resonance in a V14‐1 sub‐fraction from stem ethanolic extract, chose one for highest yield percentage, highest metabolites presence, and antimicrobial activity, showed triterpenic compounds mixture (α‐amyrin, β‐amyrin and 24‐methylenecycloartanol). Findings in this study are among the first reports for S. calophyllus, even the chemical characterization, that confirm its strong antioxidant and antibacterial activities. Further molecular composition research about parasitic plants could show how unknown compounds may combat pathogenic microorganisms.

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Screening of Antibacterial and Antioxidant Activity of Biogenically Synthesized Silver Nanoparticles from Alternaria alternata, Endophytic Fungus of Dendrophthoe falcata-a Parasitic Plant

Cited by 15 publications

References 39 publications

Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities

Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities

Synthesis of CuO and PAA-Regulated Silver-Carried CuO Nanosheet Composites and Their Antibacterial Properties

Antioxidant Activity and Phytopathogenic Control of Extracts and Fraction from Struthanthus calophyllus A.C.Sm. (Loranthaceae)

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