Screening of Antibiotic Gene Clusters in Microorganisms Isolated from Wood

Fil, Mariana; Velasco-Rodríguez, Óscar; García-Calvo, Laura; Sola‐Landa, Alberto; Barreiro, Carlos

doi:10.1007/978-1-0716-1358-0_8

Cited by 2 publications

(3 citation statements)

References 39 publications

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“…This study is focused on the detection of genes encoding PKSs and NRPSs, which are involved in the production of different secondary metabolites, such as antibiotics, and the subsequent validation by means of competitive bioassays. A straightforward method of antibiotics screening from wood was applied to two well-known groups of antibiotic producers, Actinobacteria and the Penicillium genus [47]. However, when the antibiotic production was tested in vivo by means of competitive bioassays against wood-degrading fungi, the number of confirmed positives dropped drastically.…”

Section: Discussionmentioning

confidence: 99%

“…DNA obtained from the species identification was used for polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS) detection. PCR amplification was carried out to detect PKS/NRPS genes in Actinobacteria and Penicillium species using different sets of degenerate primers, as updated by Fil et al [47]. Three different sets of primers were used for Actinobacteria (Table 3): K1F/M6R for targeting PKS I [48], KSF/KSR for targeting PKS II [49], and A3F/A7R for NRPS identification (Table 3) [48].…”

Section: Polyketide Synthases and Non-ribosomal Peptide Synthetases G...mentioning

confidence: 99%

“…PKS are multifunctional enzymes that catalyse the production of polyketides. These are a large group of bioactive secondary metabolites used as antiparasitic, immunosuppressant, antifungal, antibacterial, or antitumor drugs [47].…”

Section: Characterization Of the Isolates: Antimicrobial Potential (N...mentioning

confidence: 99%

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Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms

et al. 2022

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Rotting wood is inhabited by a large diversity of bacteria, fungi, and insects with complex environmental relationships. The aim of this work was to study the composition of the microbiota (bacteria and fungi) in decaying wood from a northwest Spanish forest as a source of industrially relevant microorganisms. The analyzed forest is situated in a well-defined biogeographic area combining Mediterranean and temperate macrobioclimates. Bacterial diversity, determined by metagenome analyses, was higher than fungal heterogeneity. However, a total of 194 different cultivable bacterial isolates (mainly Bacillaceae, Streptomycetaceae, Paenibacillaceae, and Microbacteriaceae) were obtained, in contrast to 343 fungal strains (mainly Aspergillaceae, Hypocreaceae, and Coniochaetaceae). Isolates traditionally known as secondary metabolite producers, such as Actinobacteria and members of the Penicillium genus, were screened for their antimicrobial activity by the detection of antibiotic biosynthetic clusters and competitive bioassays against fungi involved in wood decay. In addition, the ability of Penicillium isolates to degrade cellulose and release ferulic acid from wood was also examined. These results present decaying wood as an ecologically rich niche and a promising source of biotechnologically interesting microorganisms.

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Section: Discussionmentioning

confidence: 99%

Section: Polyketide Synthases and Non-ribosomal Peptide Synthetases G...mentioning

confidence: 99%

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Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms

et al. 2022

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Emerging Function of Ecotype-Specific Splicing in the Recruitment of Commensal Microbiome

Yang

Wang

et al. 2022

IJMS

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In recent years, host–microbiome interactions in both animals and plants has emerged as a novel research area for studying the relationship between host organisms and their commensal microbial communities. The fitness advantages of this mutualistic interaction can be found in both plant hosts and their associated microbiome, however, the driving forces mediating this beneficial interaction are poorly understood. Alternative splicing (AS), a pivotal post-transcriptional mechanism, has been demonstrated to play a crucial role in plant development and stress responses among diverse plant ecotypes. This natural variation of plants also has an impact on their commensal microbiome. In this article, we review the current progress of plant natural variation on their microbiome community, and discuss knowledge gaps between AS regulation of plants in response to their intimately related microbiota. Through the impact of this article, an avenue could be established to study the biological mechanism of naturally varied splicing isoforms on plant-associated microbiome assembly.

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Screening of Antibiotic Gene Clusters in Microorganisms Isolated from Wood

Cited by 2 publications

References 39 publications

Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms

Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms

Emerging Function of Ecotype-Specific Splicing in the Recruitment of Commensal Microbiome

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