Screening of Antibiotic Producing Actinomycetes for Antibiosis from Soil of Siraha, Nepal

Sah, Shiv Nandan; Lekhak, Binod

doi:10.3126/hijost.v1i0.25817

Cited by 5 publications

(6 citation statements)

References 3 publications

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“…Since the evaporation of crude filtrate was used to produce these metabolites, it was likely to have a high MIC value. Similar findings were also reported by Gurung, Sherpa, Agrawal, Lekhak, et al (2009) and Sah et al (2017). TLC showed only one antibiotic solution created the spot, indicating the existence of a single compound.…”

supporting

confidence: 90%

Screening of Antibiotic producing Actinomycetes for Antibiosis from soil of Sunsari, Nepal

Limbu,

Majhi,

Sah

et al. 2023

Himalayan J. Sci. Technol.

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Actinomycetes, slow-growing gram-positive bacteria, are useful in the search for bioactive compounds. A total of 24 different actinomycete strains were recovered from farming soil samples collected from the Sunsari district. The isolates were then tested against two gram-positive and three gram-negative bacteria. Results showed that 21% of all isolates are antibacterial at least, one of the test organisms, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Salmonella Typhi, and Pseudomonas spp. According to antibacterial activity and spectrum broadness, one of the isolates (S11) was selected for secondary screening. The minimum inhibitory concentration (MIC) of crude antibacterial substances extracted from the broth culture of the isolate (S11) was found to be 1.3 mg/ml against test organisms. The chromatogram in TLC showed only one spot with an Rf value of 0.87 by the isolate suggesting that the isolate produced only one compound which was utterly different from the spot with an Rf value of 0.94 paid by gentamycin. According to identification by Microscopy (1000X) and overall biochemical, and physiological characteristics, the isolate was considered Streptomyces antibioticus, a distinct taxonomic group.

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supporting

confidence: 90%

Screening of Antibiotic producing Actinomycetes for Antibiosis from soil of Sunsari, Nepal

Limbu,

Majhi,

Sah

et al. 2023

Himalayan J. Sci. Technol.

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“…Sah et al (2021) extracted antibacterial metabolites from the fermentation liquid using various organic solvents (n-butanol, chloroform, dichloromethane, ethyl acetate, and methanol), but only the ethyl acetate solvent was able to extract potent metabolites at a detectable level from the fermented broth. This could be attributed to the superior solubility of the metabolites in the ethyl acetate solvent (Sah and Lekhak 2017). The extracted ethyl acetate from Actinomycetes B.11 against A. niger and C. albicans, while the ethyl acetate extract from Actinomycetes B.17 only inhibits C. albicans.…”

Section: Fermentation and Extraction Of Secondary Metabolite Isolate ...mentioning

confidence: 99%

Isolation and identification of Actinomycetes with antifungal activity from karts ecosystem in Maros-Pangkep, Indonesia

RANTE,

MANGGAU,

ALAM

et al. 2024

Biodiversitas

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Abstract. Rante H, Manggau MA, Alam G, Pakki E, Erviani AE, Hafidah N, Abidin HL, Ali A. 2024. Isolation and identification of Actinomycetes with antifungal activity from karts ecosystem in Maros-Pangkep, Indonesia. Biodiversitas 25: 458-464. Actinomycetes have yielded various biologically active secondary compounds with intriguing properties like antimicrobial, antiviral, and anticancer effects. This research aimed to isolate, identify, and screen the antifungi from soil environmental samples collected from the karst ecosystem in Maros-Pangkep, Indonesia. The active isolate is then fermented for the production of secondary metabolites. The fermentation process uses an M1 medium under agitated conditions at 150 rpm for 12 days. The isolate Actinomycetes were identified based on sequence gen 16S rRNA. Screening of antifungal activity was carried out against Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404 by antagonistic test. The diffusion method was applied using the paper disc to assess the antifungal activity. The result revealed that 8 isolates were purified from the soil samples collected. From the 8 isolates of Actinomycetes obtained, two Actinomycetes exhibited antifungal activities in the screening methods, namely isolates with code B11 and B 17. The crude extract of isolate B11 was active against C. albicans and A. niger at concentrations of 2 mg/paper disc, 1.5 mg/paper disc, and 0.75 mg/paper disc. Furthermore, isolate B17 was found to be only active against C. albicans. The phylogenetic analysis of the 16S rRNA gene sequences indicated that B11 showed the highest similarity to Streptomyces tuirus strain NBRC 15617.

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“…Actinomycetes are Gram-positive bacteria that can live on a wide range of substrates; both the simple and complex ones. Several general bacterial isolation procedures can be used to isolate the actinomycetes, including serial dilution; pouring plates, streaking, and centrifugation (Majhi, 2018). In the soil samples, centrifugation followed by repeated dilutions can enhance the growth of actinomycetes.…”

Section: Isolation Of the Soil Actinomycetesmentioning

confidence: 99%

A review on Actinomycetes distribution, isolation, and their medical applications

Alenazi

Anwar

Abo-Aba

et al. 2023

Novel Research in Microbiology Journal

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Actinomycetes (Actinobacteria) are Gram-positive bacteria that grow in a variety of environments and have a filamentous shape similar to fungi. The actinomycetes distinguish themselves morphologically by forming a layer of hyphae that carry chains of spores. The aim of this study was to update the recent developments related to the actinomycetes. Streptomyces; as an actinomycete, is known for producing a number of bioactive secondary metabolites, including anti-tumour agents; antibiotics, antifungals, antivirals, anti-hypertension drugs, and immunosuppressives. In order to compete with other microorganisms, including those of the same genera, Streptomyces species produce several secondary metabolites. However, despite the discovery of antibiotics, the infectious diseases remain the secondleading cause of death worldwide. Each year, around 17 million people die from bacterial infections; mainly children and the elderly. In addition to the overuse of antibiotics, a key factor contributing to antibiotic resistance is self-medication, which reduces the lifespan of antibiotics.

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Screening of Antibiotic Producing Actinomycetes for Antibiosis from Soil of Siraha, Nepal

Cited by 5 publications

References 3 publications

Screening of Antibiotic producing Actinomycetes for Antibiosis from soil of Sunsari, Nepal

Screening of Antibiotic producing Actinomycetes for Antibiosis from soil of Sunsari, Nepal

Isolation and identification of Actinomycetes with antifungal activity from karts ecosystem in Maros-Pangkep, Indonesia

A review on Actinomycetes distribution, isolation, and their medical applications

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