Screening of antimicrobial, antioxidant properties and bioactive compounds of some edible mushrooms cultivated in Bangladesh

Abstract

BackgroundFor a long time mushrooms have been playing an important role in several aspects of the human activity. Recently edible mushrooms are used extensively in cooking and make part of new food in Bangladesh for their beneficial properties. The aim of this study is to screen some values of mushrooms used in Bangladesh.MethodsMethanolic extracts of 3 edible mushrooms (Pleurotus ostreatus, Lentinula edodes, Hypsizigus tessulatus) isolated from Chittagong, Bangladesh were used in this study. Phenolic compounds in the mushroom methanolic extracts were estimated by a colorimetric assay. The antioxidant activity was determined by radical 1, 1-diphenyl;-2-picrylhydrazyl (DPPH) radical scavenging assay. Eight microbial isolates were used for antimicrobial activity of methanolic extract of mushrooms by the agar well diffusion method with slight modification.ResultsDetermination of antimicrobial activity indicated considerable activity against all bacteria and fungi reveling zone of inhibition ranged from 7 ± 0.2 to 20 ± 0.1 mm. Minimum inhibitory concentration values of the extracts showed that they are also active even in least concentrations ranged from 1 mg/ml to 9 mg/ml. Lentinula edodes showed the best antimicrobial activity than others. Pseudomonas aeruginosa was quite resistant and Saccharomyces cerevisiae was more sensitive than others microbial isolates. Antioxidant efficiency by inhibitory concentration on 1,1-Diphenly-2-picrylhydrazyl (DPPH) was found significant when compared to standard antioxidant like ascorbic Acid . The concentration (IC50) ranged from 100 ± 1.20 to 110 ± 1.24 μg/ml. Total phenols are the major bioactive component found in extracts of isolates expressed as mg of GAE per gram of fruit body, which ranged from 3.20 ± 0.05 to 10.66 ± 0.52 mg/ml. Average concentration of flavonoid ranged from 2.50 ± 0.008 mg/ml to 4.76 ± 0.11 mg/ml; followed by very small concentration of ascorbic acid (range, 0.06 ± 0.00 mg/ml to 0.21 ± 0.01 mg/ml) in all the isolates. All the isolates showed high phenol and flavonoid content (except Pleurotus ostreatus), but ascorbic acid content was found in traces.ConclusionThis study has revealed that selected edible mushrooms have not only nutritional values but also some therapeutic values. Proper and more investigations can lead us to use these as strong medicine in future.

Antimicrobial Potential, Identification and Phylogenetic Affiliation of Wild Mushrooms from Two Sub-Tropical Semi-Evergreen Indian Forest Ecosystems

“…Similarly, a previous study has also reported antimicrobial activity from the extract of mushrooms [7]. In our study, the maximum inhibition zone was shown by Pleurotus pulmonarius (BPSM36) showing antibacterial activities against P .…”

“…Worldwide, they are well recognized as supplementary food due to their high nutritional values [1, 2] and medicinal importance, which includes their antioxidant and antimicrobial activities [3, 4], immune enhancer [5–7], and to be effective for the treatment of diabetic [8, 9] and few types of cancers as well [10,11]. …”

Detection of a Non-animal Source of Glycosaminoglycans from Edible Mushrooms in Northern Thailand

“…These findings are thought to be the consequence of using acidic water (Nakano et al, 2000) for preparing mushroom extracts, as we did in this study; this method may not be suitable for extracting tannins or other phenolic compounds of some mushroom species (Tiwari et al, 2011). We did find flavonoids in most of the mushroom extracts; this finding supported the presence of catechin in shitake mushroom, log white fungi, and log black fungi (Attarat and Phermthai, 2015;Chowdhury et al, 2015). Some studies have demonstrated that bioactive compounds in mushrooms play an essential role in the human immune system by acting as immunomodulating molecules.…”

In-vitro antimicrobial activity and complement/macrophage stimulating effects of a hot-water extract from mycelium of the oyster mushroom Pleurotus sp.

“…S. aureus ATCC 6588 was found to require an extract concentration of 12.5 mg/mL, regardless of the source of nitrogen used for the occurrence of high antimicrobial activity, and E. coli CBAB 2 proved to be resistant to these extracts. The mycelial hotwater extract evaluated here was also effective at a concentration lower than MICs reported for a methanolic extract of P. ostreatus isolated from Bangladesh, in the range of 4 mg/mL for the yeasts C. albicans and S. cerevisiae to 8 mg/mL for P. aeruginosa (Chowdhury, Kubra, & Ahme, 2015).…”