Screening of bacteria of the genus<i>Bacillus</i>for the control of the plant-pathogenic fungus<i>Macrophomina phaseolina</i>

Marroni, Igor Villela

doi:10.1080/09583157.2014.976178

Cited by 1 publication

(1 citation statement)

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“…[1] Bacillus amyloliquefaciens PEBA20 inhibited mycelial growth and spore germination of pathogens as well as reduced fungal infections in wheat and maize. [2] Marroni [3] selected Bacillus RP5 as biocontrol inoculum for the plant-pathogenic fungus, Macrophomina phaseolina. In our previous works, Bacillus subtilis SL-13 with antifungal chitinase was obtained from cotton field soil in Xinjiang province.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of alginate–gelatin microencapsulatedBacillus subtilisSL-13 by emulsification/internal gelation

Liang

Yang

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Gelatin was blended with sodium alginate (NaALG) to obtain a novel microbial fungicide, and dispersed micron Bacillus subtilis SL-13 microspheres prepared by emulsification/internal gelation method. Microscopic examination revealed that microcapsules were nearly spherical in shape. Fourier transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction confirmed that the electrostatic interaction was occurred when gelatin added into NaALG. The maximum encapsulation efficiency was 93.44% at a gelatin concentration of 1.5%. Particle size, swelling, and biodegradation of beads increased with gelatin content increase. Furthermore, the viability of encapsulated SL-13 could be preserved at more than 10(8) CFU/mL after 120 d storage at 25 °C. The number of viable cells released from microcapsules presented an initial rapid increase followed by a gradual increase, and reached the maximum as 10(10) CFU/mL on day 35. Thus, it is feasible to prepare uniform, rounded shape, and well-dispersed micron microcapsules of SL-13 via emulsification/internal gelation using NaALG and gelatin composites. This encapsulation strategy could be considered as a potential alternative to future applications in the agricultural industry.

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Section: Introductionmentioning

confidence: 99%