Screening of Bacteria Producing Lipase from Insect Gut: Isolation and Characterization of a Strain, Burkholderia sp. HY-10 Producing Lipase

Park, Doo-Sang; Oh, Hyun‐Woo; Bae, Kyung-Sook; Kim, Hyang-Mi; Heo, Sun-Yeon; Kim, Nam‐Jung; Seol, Kwang-Youl; Park, Ho-Yong

doi:10.5656/ksae.2007.46.1.131

Cited by 6 publications

(3 citation statements)

References 15 publications

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“…are important sources of lipase production, but several other microbes are used as well (Pandey et al 1999 ). Symbiotic microorganisms with potential lipase and/or protease activity have been isolated from insects, including Bacillus , Staphylococcus , Pseudomonas , Fusarium solani , Candida fermentati , Yarrowia lipolytica , and yeast-like symbionts from silkworm, long-horned beetles, cigarette beetles, cottony maple scale insects, and burying beetles, respectively (Brues and Glaser 1921 ; Vega and Dowd 2005 ; Park et al 2007 ; Feng et al 2011 ; Scully et al 2012 ; Kaltenpoth and Steiger 2014 ). Unfortunately, however, information on their relevance for host fitness or their potential for biotechnological application remains limited.…”

Section: Introductionmentioning

confidence: 99%

Potential applications of insect symbionts in biotechnology

Berasategui

Shukla

Salem

et al. 2015

Appl Microbiol Biotechnol

123

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Symbiotic interactions between insects and microorganisms are widespread in nature and are often the source of ecological innovations. In addition to supplementing their host with essential nutrients, microbial symbionts can produce enzymes that help degrade their food source as well as small molecules that defend against pathogens, parasites, and predators. As such, the study of insect ecology and symbiosis represents an important source of chemical compounds and enzymes with potential biotechnological value. In addition, the knowledge on insect symbiosis can provide novel avenues for the control of agricultural pest insects and vectors of human diseases, through targeted manipulation of the symbionts or the host-symbiont associations. Here, we discuss different insect-microbe interactions that can be exploited for insect pest and human disease control, as well as in human medicine and industrial processes. Our aim is to raise awareness that insect symbionts can be interesting sources of biotechnological applications and that knowledge on insect ecology can guide targeted efforts to discover microorganisms of applied value.

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Section: Introductionmentioning

confidence: 99%

Potential applications of insect symbionts in biotechnology

Berasategui

Shukla

Salem

et al. 2015

Appl Microbiol Biotechnol

123

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“…The isolation of intestinal xylan-degrading microorganisms from G. orientalis was carried out using an M9 solid medium that contained 0.3% yeast extract and 0.5% birchwood xylan, as described elsewhere (Park et al 2007). Of the microorganisms grown on the M9 solid medium, strain HY-12 formed a large translucent halo around its colony and was therefore selected for further study.…”

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confidence: 99%

Biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of Gryllotalpa orientalis, Cellulosimicrobium sp. HY-12

Heo

Kim

et al. 2007

Antonie van Leeuwenhoek

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An exo-symbiotic bacterium capable of hydrolyzing xylan was isolated from the gut of the mole cricket, Gryllotalpa orientalis, and identified as Cellulosimicrobium sp. HY-12. The xylanase (XylA( CspHY-12)) of this organism bound tightly to both DEAE and mono Q resins, and its molecular mass (M(r)) was about 39.0 kDa. The highest xylanase activity was observed at pH 6.0 and 60 degrees C. The enzyme was greatly suppressed by Ca(2+), Cu(2+), Co(2+), and Fe(2+) ions but not by Mg(2+) and Mn(2+). Although XylA( CspHY-12) was capable of hydrolyzing various types of xylosic compounds, it could not decompose carboxymethyl cellulose or xylobiose. The xylA (CspHY-12 ) gene consisted of an 1,188 bp open reading frame that encoded a polypeptide of 395 amino acids with a deduced molecular mass of 42,925 Da. The domain structure of XylA( CspHY-12) was most similar to those of the glycoside hydrolase (GH) family 10 endoxylanases. However its sequence identity with any of the enzymes in this family was below 52%. The results of this study suggest that the XylA( CspHY-12) is a new cellulase-free endo-beta-1,4-xylanase with some properties that are distinct from those of GH family 10.

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“…For example, lipases from Lactobacillus sp. G5 initially purified by ion exchange chromatography have a molecular weight of 27 kDa (Park et al, 2007), lipases from Enterococcus feacium are estimated to be 19.172 kDa (Ramakrishnan et al, 2016), tributyrinesterases from Lactobacillus plantarum strains purified and analyzed by SDS-PAGE electrophoresis are 31 kDa (Esteban-Torres et al, 2014), and Propionibacterium freudenreichii exhibits intracellular esterases of 52 kDa (Kakariari et al, 2000).…”

Section: Sephadex-g100 Gel Filtration Chromatographymentioning

confidence: 99%

Characterization of lipases and preliminary purification of tributyrinesterases from lactic acid bacteria strains belonging to the Enterococcus and Lactococcus genera

DELLALI,

BOUBLENZA,

ZADI-KARAM

et al. 2023

Not Sci Biol

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The objective of this study is to characterize and attempt to purify lipolytic enzymes derived from strains of Enterococcus and Lactococcus genera isolated from various environments. For this purpose, ten strains of lactic acid bacteria were studied for their lipolytic and esterasic activities on an MRS (Man, Rogosa, and Sharpe) medium containing natural and/or artificial lipid substrates. Among them, two strains: Lactococcus lactis subsp lactis and Lactococcus lactis subsp cremoris showed maximum extracellular lipolytic activity in the presence of 1% olive oil. It was observed that both strains exhibited higher lipolytic activity at pH 7 and 8, with an optimal temperature of 30 °C and 37 °C, respectively. Glucose was found to be the best carbon source for both tested strains. The study of growth kinetics and fatty acid production over time revealed that fatty acid production begins during the exponential phase and reaches its maximum during the stationary phase. The purification of tributyrinesterases was carried out on two strains of Enterococcus genus: Enterococcus feacium and Enterococcus durans through ammonium sulfate precipitation and Sephadex G-100 gel filtration chromatography allowed the estimation of the molecular weight of the tributyrinesterases as 32.09 kDa and 38.49 kDa for both strains, respectively.

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Screening of Bacteria Producing Lipase from Insect Gut: Isolation and Characterization of a Strain, Burkholderia sp. HY-10 Producing Lipase

Cited by 6 publications

References 15 publications

Potential applications of insect symbionts in biotechnology

Potential applications of insect symbionts in biotechnology

Biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of Gryllotalpa orientalis, Cellulosimicrobium sp. HY-12

Characterization of lipases and preliminary purification of tributyrinesterases from lactic acid bacteria strains belonging to the Enterococcus and Lactococcus genera

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