Screening of bacterial antagonists for biological control ofPhytophthora blight of pepper

Abstract

The aim of this study was to assess the potential of bacterial antagonists to control Phytophthora blight of pepper caused by P. capsici using different screening methods. Among a collection of fluorescent pseudomonas isolated from the rhizosphere of pepper, twelve isolates were initially selected based on dual culture assay on potato dextrose agar and corn meal agar. Further, these twelve isolates were screened for the reduction of disease severity caused by P. capsici using detached leaves and seedling assay. Most of the antagonists showed varying levels of antagonism against P. capsici in both detached leaves and seedlings assay. In addition, few isolates increased shoot and root length of pepper in seedling assays. Among them, isolate PS119 showing highest ability to reduce the disease severity in the in vitro seedling assay was found to be the most efficient antagonists against P. capsici in the in vivo biological control tests. These results indicate that the in vitro seedling assay can be used as a rapid and more accurate technique for the selection of promising biocontrol agents against P. capsici.

The Effect of Blue-light-emitting Diodes on Antioxidant Properties and Resistance to Botrytis cinerea in Tomato

“…The leaves were inoculated with 10 µL drops of the B. cinerea spore suspension (5.7×10 5 mL -1 ), and then kept under blue or broad-spectrum-white LED light conditions for 10 days. Disease severity on leaves infected with B. cinerea was visibly assessed on a scale of 0 ('no symptoms') to 4 ('51 to 100% symptoms'), according to the method of Rajkumar et al [30].…”

Phytophthora capsici on chilli pepper (Capsicum annuum L.) and its management through genetic and bio-control: a review

“…Plant growth-promoting rhizobacteria (PGPR) not only increased the production of pepper Reddy et al, 2016), but Bacillus vallismortis strain BS07 and B. subtilis CAS15 were found as a potential bio-control agent particularly against Phytophathora blight, Fusarium wilt and anthracnose disease of chilli plants. Plants treated with this rhizobacterium also showed significant boost in chlorophyll contents of leaves and increased fruit yield (Rajkumar et al, 2005).…”

“…Plants treated with this rhizobacterium also showed significant increase in chlorophyll contents of leaves and increased fruit yield. SB10 also has showed 72.2% ability to decelerate disease incidence in chilli plants (Rajkumar et al, 2005;Jiang et al, 2006).…”

Scientifically advanced solutions for chestnut ink disease

“…Whether the reduced disease is due to decreased pathogen activity or to induced resistance in the plant, or whether there are other mechanisms operating to suppress the pathogen, is still not fully understood. A number of study shows that antagonistic microbes are potential as biocontrol agent against plant pathogen (Hoopen et al 2003;Lourenço et al 2004;Okamoto et al 2000;Rajkumar et al 2005;Sid et al 2003). Other studies on pot trials also indicate some potential biocontrol agents against Phytophthora.…”