Screening of banana bunchy top virus through multiplex PCR approach

Chandrasekar, A.; Kalaimughilan, Kalaiponmani; Elayabalan, Sivalingam; Kumar, K. K.; Angappan, K.; Balasubramanian, P.

doi:10.1080/03235408.2010.522820

Cited by 6 publications

(3 citation statements)

References 5 publications

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“…The viral infection also changes the nutritional quality of the plant and its attractiveness for insects, including those vectors of viruses (Schoelz andStewart 2018, Safari Murhububa et al 2021). The banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae), is the vector of the Banana bunchy top virus (BBTV), the causal agent of banana bunchy top disease, which is currently the most serious viral infection of banana plants worldwide (Thomas and Iskra-Caruana 2000, Chandrassekar et al 2011, Qazi 2016). The disease is characterized by dwarfed and narrow leaves, chlorosis of leaf margins, and dark-green discontinuous streaks on leaves, petioles, and pseudostem.…”

Section: Introductionmentioning

confidence: 99%

The aphidPentalonia nigronervosa(Hemiptera: Aphididae) takes advantage from the quality change in banana plant associated withBanana bunchy top virusinfection

Murhububa

Tougeron

Bragard

et al. 2023

Journal of Economic Entomology

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Viral diseases can change plant metabolism, with potential impacts on the quality of the plant’s food supply for insect pests, including virus vectors. The banana aphid, Pentalonia nigronervosa Coquerel, is the vector of the Banana bunchy top virus (BBTV), the causal agent of Banana bunchy top disease (BBTD), the most devastating viral disease of bananas in the world. The effect of BBTV on the life-history traits and population dynamics of P. nigronervosa remains poorly understood. We therefore studied the survival rate, longevity, daily fecundity per aphid, tibia length, population growth, and winged morph production of a P. nigronervosa clone grown on healthy or infected, dessert, or plantain banana plants. We found that daily fecundity was higher on infected banana than on healthy banana plants (plantain and dessert), and on plantain than on dessert banana plants (healthy and infected). Survival and longevity were lower on infected dessert bananas than on other types of bananas. In addition, virus infection resulted in a decrease in aphid hind tibia length on both plant genotypes. The survival and fecundity table revealed that the aphid net reproduction rate (Ro) was highest on plantains (especially infected plantain), and the intrinsic growth rate (r) was highest on infected plants. Finally, the increase of aphids and alate production was faster first on infected plantain, then on healthy plantain, and lower on dessert banana (infected and uninfected). Our results reinforce the idea of indirect and plant genotype-dependent manipulation of P. nigronervosa by the BBTV.

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Section: Introductionmentioning

confidence: 99%

The aphidPentalonia nigronervosa(Hemiptera: Aphididae) takes advantage from the quality change in banana plant associated withBanana bunchy top virusinfection

Murhububa

Tougeron

Bragard

et al. 2023

Journal of Economic Entomology

View full text Add to dashboard Cite

show abstract

“…Most of the recent literature showed that BBTV detection was effective using PCR method (Mansoor et al, 2005). PCR based detection technique, had the advantage of amplifying the target nucleic acid present even at very low level and it had become an attractive technique for the diagnosis of many plant viral diseases (Henson and French, 1993;Xie and Hu, 1994;Hafner et al, 1997a, Chandrasekar et al, 2011.…”

Section: Expression Of Bbtv Coat Protein In E Colimentioning

confidence: 99%

“…BBTV detection method using internal control primer and BBTV Rep gene primer was reported by Mansoor et al, (2005). Potential problems in PCR include false negatives due to reaction failure or false positives due to contamination (Chandrasekar et al, 2011). False negatives were often revealed in Southern blot technique.…”

Section: Expression Of Bbtv Coat Protein In E Colimentioning

confidence: 99%