Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients

Menzyanova, N. G.; Pyatina, Svetlana; Nikolaeva, Elena D.; Shabanov, A. V.; Немцев, И. В.; Stolyarov, Dmitry P.; Dryganov, Dmitry B.; Sakhnov, Eugene V.; Shishatskaya, Ekaterina I.

doi:10.1016/j.toxrep.2018.11.009

Cited by 5 publications

(2 citation statements)

References 84 publications

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“…Atherosclerosis is a severe disease originating in the blood vessels. 24 During the occurrence and development of atherosclerosis, instability and detachment of the atherosclerotic plaque on the inner surface of blood vessels leads to more serious circulatory diseases, including thrombosis and coronary artery cell damage. 8 , 25 , 26 However, apoptosis of VSMCs can lead to instability and rupture of the atherosclerotic plaque.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA PPP1CC promotes Porphyromonas gingivalis-lipopolysaccharide-induced pyroptosis of vascular smooth muscle cells by activating the HMGB1/TLR9/AIM2 pathway

Liu¹,

Wang²,

Liao³

et al. 2021

J Int Med Res

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Objective Porphyromonas gingivalis ( Pg) plays a critical role in the occurrence and development of atherosclerosis. Lipopolysaccharide from Pg ( Pg-LPS) could lead to pyroptosis of vascular smooth muscle cells (VSMCs) and induce instability of atherosclerotic plaque. Therefore, pyroptosis of VSMCs could promote the process of atherosclerosis. However, the exact mechanism of Pg-LPS-induced pyroptosis of VSMCs is unclear. Methods We determined pyroptosis and expression of interleukin (IL)-1β and IL-18 in VSMCs using 4′,6-diamidino-2-phenylindole staining and ELISA after stimulation by Pg-LPS. We established a knockdown plasmid containing the circular (circ)RNA PPP1CC and transfected it into VSMCs. Luciferase assays were performed to reveal the association between microRNAs miR-103a-3p and miR-107 and circRNA PPP1CC. Results Stimulation of Pg-LPS led to pyroptosis of VSMCs. Knockdown of circRNA PPP1CC relieved the Pg-LPS-induced pyroptosis of VSMCs and suppressed the expression of HMGB1, TLR9, AIM2, and cleaved caspase-1. Luciferase assays showed that PPP1CC directly targeted and competitively adsorbed miR-103a-3p and miR-107, weakening the inhibitory effect of these microRNAs on the expression of HMGB1. Conclusion Knockdown of circRNA PPP1CC relieved Pg-LPS-induced pyroptosis of VSMCs. Pyroptosis of VSMCs appears to promote atherosclerosis and may represent a novel therapeutic target for its treatment.

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Section: Discussionmentioning

confidence: 99%

Circular RNA PPP1CC promotes Porphyromonas gingivalis-lipopolysaccharide-induced pyroptosis of vascular smooth muscle cells by activating the HMGB1/TLR9/AIM2 pathway

Liu¹,

Wang²,

Liao³

et al. 2021

J Int Med Res

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“…It is known that the inclusion of such monomers as 4-hydroxybutyrate (4HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) affects the physical and mechanical properties [ 47 , 48 , 49 ]. Features of the microstructure and surface relief of PHA samples of different monomeric composition depend on the inclusion of these monomers, which affect the biological properties [ 50 , 51 ]. A thermoplastic polyester, poly-3-hydroxybutyrate (P3HB), has almost the same properties as polypropylene.…”

Section: Introductionmentioning

confidence: 99%

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

Ryltseva

Dudaev

Menzyanova

et al. 2023

JFB

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The needs of modern regenerative medicine for biodegradable polymers are wide and varied. Restoration of the viability of the vascular tree is one of the most important components of the preservation of the usefulness of organs and tissues. The creation of vascular implants compatible with blood is an important task of vascular bioengineering. The function of the endothelial layer of the vessel, being largely responsible for the development of thrombotic complications, is of great importance for hemocompatibility. The development of surfaces with specific characteristics of biomaterials that are used in vascular technologies is one of the solutions for their correct endothelialization. Linear polyhydroxyalkanoates (PHAs) are biodegradable structural polymeric materials suitable for obtaining various types of implants and tissue engineering, having a wide range of structural and physicomechanical properties. The use of PHA of various monomeric compositions in endothelial cultivation makes it possible to evaluate the influence of material properties, especially surface characteristics, on the functional state of cells. It has been established that PHA samples with the inclusion of 3-hydroxyhexanoate have optimal characteristics for the formation of a human umbilical vein endothelial cell, HUVEC, monolayer in terms of cell morphology as well as the levels of expression of vinculin and VE-cadherin. The obtained results provide a rationale for the use of PHA copolymers as materials for direct contact with the endothelium in vascular implants.

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Investigating the anti-bioadhesion properties of short, medium chain length, and amphiphilic polyhydroxyalkanoate films

Guennec,

Balnois,

Augias

et al. 2024

Biofouling

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Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients

Abstract: Graphical abstract

Cited by 5 publications

References 84 publications

Circular RNA PPP1CC promotes Porphyromonas gingivalis-lipopolysaccharide-induced pyroptosis of vascular smooth muscle cells by activating the HMGB1/TLR9/AIM2 pathway

Circular RNA PPP1CC promotes Porphyromonas gingivalis-lipopolysaccharide-induced pyroptosis of vascular smooth muscle cells by activating the HMGB1/TLR9/AIM2 pathway

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

Investigating the anti-bioadhesion properties of short, medium chain length, and amphiphilic polyhydroxyalkanoate films

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