Screening of Biotechnological Parameters for Fructofuranosidases Production by a Newly Isolated Fungal Strain Using Plackett-Burman Design

Bonciu, Camelia; Constantin, Oana Emilia; Bahrim, Gabriela

doi:10.15835/nbha3926323

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…The experimental conditions for medium composition selected for the present study were a carbon: nitrogen ratio between 0·4 and 10 and a temperature range between 10 and 30°C (Tables 1 and 2). These values were obtained in a previous study, in which the carbon/nitrogen ratio in the fermentative medium and temperature were identified as the most important factors affecting extracellular inulinase production by the mould strain Rhizoctonia I‐PN4 (Neagu Bonciu et al. 2011).…”

Section: Resultssupporting

confidence: 56%

“…strain, coded I‐PN4, from the Industrial Microbiology Collection of Microorganisms of Research Platform Bioaliment, Dunarea de Jos University, Galati, Romania, previously selected as a good producer of inulinase, was isolated from soil from Jerusalem artichoke tubers (Bonciu et al. 2010; Neagu Bonciu et al. 2011).…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, the most important factors affecting the inulinase biosynthesis by the newly isolated fungal strain Rhizoctonia ssp. were identified using Plackett–Burman design (Neagu Bonciu et al. 2011).…”

Section: Introductionmentioning

confidence: 99%

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Increase in extracellular inulinase production for a new Rhizoctonia ssp. strain by using buckwheat (Fagopyrum esculentum) flour as a single carbon source

Bonciu¹,

Constantin²,

Bahrim³

2012

Letters in Applied Microbiology

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Aims: A newly isolated strain of Rhizoctonia ssp. was used for the production of extracellular inulinase. Previously, the qualitative effects of some carbon and nitrogen sources from fermentative media and the physicochemical parameters for growth were established by Plackett-Burman analysis, and the main parameters that affect extracellular inulinase yield were identified. In this study, the quantitative effect of the carbon to nitrogen ratio in the fermentative medium and the growth temperature were studied and optimized using central composite design and response surface methodology. Methods and results: On the basis of optimization, the maximum extracellular inulinase activity was achieved when 2AE5-6AE5% buckwheat flour was used as a single carbon source and 4AE6-5AE0% yeast extract was used as nitrogen source, by submerged cultivation, after 48 h at an incubation temperature between 15 and 27AE5°C. Conclusions: Under the fermentative conditions established in this study, a maximum extracellular inulinase yield of 1AE8 UI ml )1 was achieved. Rhizoctonia ssp. strain can be used for extracellular inulinase production. Also, buckwheat flour proved to be an inexpensive and abundant substrate suitable for obtaining inulinase.Significance and impact of the study: Inulinases are versatile tools for biotechnology as they can be used for a wide range of applications, including production of bioethanol, fructose syrup and inulo-oligosaccharides, lactic acid, citric acid and butanediol. Letters in Applied Microbiology

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Section: Resultssupporting

confidence: 56%

Section: Methodsmentioning

confidence: 99%

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Increase in extracellular inulinase production for a new Rhizoctonia ssp. strain by using buckwheat (Fagopyrum esculentum) flour as a single carbon source

Bonciu¹,

Constantin²,

Bahrim³

2012

Letters in Applied Microbiology

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Bio-utilization of agricultural residue banana plant shoot through solid state fermentation for production of inulinase using newly isolated Nothophoma anigozanthi JAM

Tippanavar

Bhat

Rebello

et al. 2023

Biomass Conv. Bioref.

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The present research was carried out with the objectives of isolating fungal strains capable of exhibiting inulinase activity from a novel source, then optimizing solid state fermentation for the inulinase production and characterizing crude enzyme. Production of inulinase is first reported in this study with a novel substrate, banana plant shoot powder, using newly isolated fungal strain Nothophoma anigozanthi JAM through solid state fermentation. The fungal strain was isolated from the root soil of a white Jamun (Syzygium cumini) tree. Optimization of the solid state fermentation was carried out by central composite design for determining the interaction and impact of the influential process on inulinase production. A mathematical model was developed based on the statistical analysis and recommended optimal process conditions of 2 g of substrate, 5 mL of moistening media, 5 mL of inoculum volume, and 96 h of fermentation time for enhanced production (2.578 U/gds). Crude inulinase enzyme exhibited an 8.0-fold increase in activity after optimization, in comparison with the activity under unoptimized conditions. The optimum pH (5.0), temperature (60 °C) for the enzyme activity, and Km (0.205 mM) and Vmax (0.333 µM/min) were determined based on the biochemical and inulin hydrolytic properties of the crude inulinase enzyme. The production of inulinase was further validated by using TLC, osazone, and Seliwanoff tests. Hence, the inulinase enzyme produced by Nothophoma anigozanthi JAM can be successfully utilized for the production of edible sugar fructose from non-edible polysaccharide inulin, present in agricultural residues such as banana shoot by solid state fermentation.

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Status and Future Prospects of Fructooligosaccharides as Nutraceuticals

Kumar

Sripada

Poornachandra

2018

Role of Materials Science in Food Bioengineering

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Screening of Biotechnological Parameters for Fructofuranosidases Production by a Newly Isolated Fungal Strain Using Plackett-Burman Design

Cited by 5 publications

References 23 publications

Increase in extracellular inulinase production for a new Rhizoctonia ssp. strain by using buckwheat (Fagopyrum esculentum) flour as a single carbon source

Increase in extracellular inulinase production for a new Rhizoctonia ssp. strain by using buckwheat (Fagopyrum esculentum) flour as a single carbon source

Bio-utilization of agricultural residue banana plant shoot through solid state fermentation for production of inulinase using newly isolated Nothophoma anigozanthi JAM

Status and Future Prospects of Fructooligosaccharides as Nutraceuticals

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