Screening of Brazilian soybean genotypes with high potential for somatic embryogenesis and plant regeneration

Droste, Annette; Silva, Anelise Machado da; Souza, Iliane Freitas de; Wiebke-Strohm, Beatriz; Bücker-Neto, Lauro; Bencke, Marta; Sauner, Márcia Viviane; Bodanese‐Zanettini, Maria Helena

doi:10.1590/s0100-204x2010000700012

Cited by 6 publications

(13 citation statements)

References 23 publications

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“…Subsequently, several papers have reported the development of somatic embryos from cotyledons of immature embryos. But until today the measured frequencies at which these embryos convert into plants are significantly lower than that expected (Parrott et al, 1988;Meurer et al, 2001;Droste et al, 2001Droste et al, , 2010. Therefore, efforts have been directed towards developing and refining protocols for initiation, proliferation, and histodifferentiation/maturation of soybean somatic embryogenesis.…”

Section: Optimization Of Soybean Somatic Embryogenesis Protocolmentioning

confidence: 97%

“…In soybean, considerable variation in embryogenic capacity was found to exist between individual genotypes (Komatsuda et al, 1991;Bailey et al, 1993a, b;Santos et al, 1997;Droste et al, 2001;Meurer et al, 2001;Tomlin et al, 2002;Hiraga et al, 2007;Yang et al, 2009;Droste et al, 2010) as will be discussed below (Genotype-dependent response and screening of highly responsive cultivars section).…”

Section: Somatic Embryo Inductionmentioning

confidence: 99%

“…Studies with soybean plants indicated that germination/conversion capacity is also greatly influenced by genotype (Komatsuda & Ohyama, 1988;Bailey et al, 1993;Santos et al, 1997;Droste et al, 2001Droste et al, , 2010.…”

Section: Embryo Germination and Conversion Into Plantsmentioning

confidence: 99%

“…Several studies have revealed the differences among soybean genotypes in their capacity to respond to the different steps of somatic embryogenesis (Bailey et al, 1993;Santos et al, 1997;Simmonds & Donaldson, 2000;Droste et al, 2001;Meurer et al, 2001;Tomlin et al, 2002;Hiraga et al, 2007;Yang et al, 2009;Droste et al, 2010). The efficiencies were shown to be different among cultivars at each SE phase: induction, proliferation, histodifferentiation/maturation, germination and conversion (Bailey et al, 1993;Santos et al, 1997).…”

Section: Genotype-dependent Response and Screening Of Highly Responsimentioning

confidence: 99%

“…Other studies also reported the high capacity of IAS-5 to produce somatic embryos (Santos et al, 1997;Di Mauro et al, 2000;Droste et al, 2001). More recently, Droste et al (2010) identified Brazilian soybean genotypes with potential to respond to in vitro culture stimuli for somatic embryo induction, embryo proliferation and plant regeneration. Somatic embryos were induced in all eight tested genotypes, but differences were observed at each stage.…”

Section: Genotype-dependent Response and Screening Of Highly Responsimentioning

confidence: 99%

See 4 more Smart Citations

Strategies for Improvement of Soybean Regeneration via Somatic Embryogenesis and Genetic Transformation

Wiebke-Strohm¹,

Homrich²,

Weber³

et al. 2012

Genetic Engineering - Basics, New Applications and Responsibilities

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Section: Optimization Of Soybean Somatic Embryogenesis Protocolmentioning

confidence: 97%

Section: Somatic Embryo Inductionmentioning

confidence: 99%

Section: Embryo Germination and Conversion Into Plantsmentioning

confidence: 99%

Section: Genotype-dependent Response and Screening Of Highly Responsimentioning

confidence: 99%

Section: Genotype-dependent Response and Screening Of Highly Responsimentioning

confidence: 99%

See 3 more Smart Citations

Strategies for Improvement of Soybean Regeneration via Somatic Embryogenesis and Genetic Transformation

Wiebke-Strohm¹,

Homrich²,

Weber³

et al. 2012

Genetic Engineering - Basics, New Applications and Responsibilities

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Transgenic fertile soybean plants derived from somatic embryos transformed via the combined DNA-free particle bombardment and Agrobacterium system

et al. 2010

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An Agrobacterium-mediated transformation procedure for soybean [Glycine max L. Merrill] proliferating somatic embryos is here described. The Agrobacterium tumefaciens LBA4404 strain harboring pTOK233, pCAMBIA1390-olp or pH7WG2D wrky plasmids was used to mediate gene transfer into the plant genome. Prior to Agrobacterium inoculation, proliferative soybean embryogenic clusters were microwounded by DNA-free tungsten particle bombardment. Three independent transformation experiments were performed. In Experiment I, 26 transgenic plants were obtained from a unique clone of cv Bragg, while 580 plants were recovered from 105 clones of cv IAS5. In Experiment II, a single hygromycin-resistant clone of cv BRSMG68 Vencedora was recovered and gave rise to five plants. In Experiment III, 19 plants of cv Bragg and 48 plants of IAS5 were recovered, representing five and 14 independent transformation events, respectively. PCR and Southern analyses confirmed the transgenes' integration into plant genomes. Transgenic plants were fertile. They flowered, set pods and seeds. Transgene segregation in two T 1 progenies fits the Mendelian pattern (3:1 transgenic:non-transgenic plants). This is the first report of transgenic fertile soybean plants obtained from somatic embryogenic tissues transformed by the system that combines DNA-free particle bombardment and Agrobacterium.

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High frequency regeneration in soybean [Glycine max (L.) Merrill.] through direct somatic embryogenesis from immature cotyledons

Huynh

Lal

Singh

et al. 2015

Ind J Plant Physiol.

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A simple and efficient system of soybean regeneration protocol was standardized through direct somatic embryogenesis by using immature cotyledons as explant. Three genotypes, namely, DS 2706, Pusa 5 and PS 1477 were selected for the evaluation of their capacity for in vitro somatic embryogenesis and plantlet regeneration. The medium MSB5 (MS macro ? micro ? B5 organics) supplemented with different concentrations of plant growth regulators (2, 4-D, NAA, BAP and ABA) were evaluated for somatic embryo initiation and subsequent development. Somatic embryos were initiated in the shortest duration (25.1 days) with the highest response (86.5 %) on MSB5 medium supplemented with 40 mg l -1 2, 4-D compared to lower concentrations or combination treatments with NAA and BAP. Somatic embryos proliferation was highest (76.25 %) in the MSB5 medium supplemented with 20 mg l -1 2, 4-D and 13 mg l -1 ABA. Somatic embryos and their morphological development were noted on medium supplemented with 6 % maltose and 0.5 % activated charcoal (79.47 %) compared to sucrose. Maltose at 6 % gave good maturation of somatic embryos (92.67 %). The highest germination (53.03 %) of somatic embryos into plantlets was achieved in the medium containing 1.5 % sucrose. Amongst the genotypes screened, DS 2706 was the most responsive. The protocol developed can be used for direct regeneration via somatic embryogenesis in different genotypes, which could also be used for attempting genetic transformation.

show abstract

Screening of Brazilian soybean genotypes with high potential for somatic embryogenesis and plant regeneration

Cited by 6 publications

References 23 publications

Strategies for Improvement of Soybean Regeneration via Somatic Embryogenesis and Genetic Transformation

Strategies for Improvement of Soybean Regeneration via Somatic Embryogenesis and Genetic Transformation

Transgenic fertile soybean plants derived from somatic embryos transformed via the combined DNA-free particle bombardment and Agrobacterium system

High frequency regeneration in soybean [Glycine max (L.) Merrill.] through direct somatic embryogenesis from immature cotyledons

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