Screening of by-products from the food industry as growth promoting agents in serum-free media for skeletal muscle cell culture

Andreassen, R. Christel; Pedersen, Mona Elisabeth; Kristoffersen, Kenneth Aase; Rønning, Sissel Beate

doi:10.1039/c9fo02690h

Cited by 63 publications

(33 citation statements)

References 39 publications

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“…Although substantial progress has been achieved in recent years by both for‐profit companies and academic groups, CM remains a relatively new area of investigation. As might be expected for a nascent field that draws heavily on knowledge gained from previous work aimed at a distinct set of problems, review, and perspective papers about CM (at least 50 by our estimate) [ 10–14,16,18,23,28,37,44,64–109 ] currently outnumber original research papers where CM production is the primary intended application (at least 24) [ 15,59,110–131 ] or CM‐related patents (at least 24). [ 132–155 ] These estimates do not include LCA or TEA studies, consumer acceptance studies, or purely computational analyses.…”

Section: Cultivated Meatmentioning

confidence: 93%

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Bomkamp¹,

Skaalure²,

Fernando³

et al. 2021

Advanced Science

159

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Cultivating meat from stem cells rather than by raising animals is a promising solution to concerns about the negative externalities of meat production. For cultivated meat to fully mimic conventional meat's organoleptic and nutritional properties, innovations in scaffolding technology are required. Many scaffolding technologies are already developed for use in biomedical tissue engineering. However, cultivated meat production comes with a unique set of constraints related to the scale and cost of production as well as the necessary attributes of the final product, such as texture and food safety. This review discusses the properties of vertebrate skeletal muscle that will need to be replicated in a successful product and the current state of scaffolding innovation within the cultivated meat industry, highlighting promising scaffold materials and techniques that can be applied to cultivated meat development. Recommendations are provided for future research into scaffolds capable of supporting the growth of high-quality meat while minimizing production costs. Although the development of appropriate scaffolds for cultivated meat is challenging, it is also tractable and provides novel opportunities to customize meat properties.

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Section: Cultivated Meatmentioning

confidence: 93%

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Bomkamp¹,

Skaalure²,

Fernando³

et al. 2021

Advanced Science

159

View full text Add to dashboard Cite

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“…However, the potential cytotoxicity and safety of microbe-based hydrolysate used has not been thoroughly tested and remains a safety concern. In another study, pork plasma digested with Alcalase was shown to restore bovine skeletal muscle cell function and enhanced cell growth (Andreassen et al 2020 ). However, the use of animal-derived hydrolysate for CM production is counterintuitive to the purpose of being slaughter-free meat itself.…”

Section: The Use Of Hydrolysate In Animal Cell Culturementioning

confidence: 99%

Applications and analysis of hydrolysates in animal cell culture

Lim

et al. 2021

Bioresour. Bioprocess.

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Animal cells are used in the manufacturing of complex biotherapeutic products since the 1980s. From its initial uses in biological research to its current importance in the biopharmaceutical industry, many types of culture media were developed: from serum-based media to serum-free to protein-free chemically defined media. The cultivation of animal cells economically has become the ultimate goal in the field of biomanufacturing. Serum serves as a source of amino acids, lipids, proteins and most importantly growth factors and hormones, which are essential for many cell types. However, the use of serum is unfavorable due to its high price tag, increased lot-to-lot variations and potential risk of microbial contamination. Efforts are progressively being made to replace serum with recombinant proteins such as growth factors, cytokines and hormones, as well as supplementation with lipids, vitamins, trace elements and hydrolysates. While hydrolysates are more complex, they provide a diverse source of nutrients to animal cells, with potential beneficial effects beyond the nutritional value. In this review, we discuss the use of hydrolysates in animal cell culture and briefly cover the composition of hydrolysates, mode of action and potential contaminants with some perspectives on its potential role in animal cell culture media formulations in the future.

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“…Conditioned media systems have also been suggested as a solution to replace serum—the growth factors and other useful products that the cultivated meat cells produce themselves can be recycled and concentrated to support growth of other new cells in an interconnected system of bioreactors (Pawitan, 2014). As will be discussed in more detail in a later section, there is the possibility that plant or fungal protein extracts can function as homologs for important growth factors and other regulatory proteins in cultivated meat media, but this remains to be investigated fully (Andreassen, Pedersen, Kristoffersen, & Beate Rønning, 2020; Venâncio et al., 2003).…”

Section: Strategies For Serum‐free Media Formulationmentioning

confidence: 99%

Considerations for the development of cost‐effective cell culture media for cultivated meat production

O’Neill

Cosenza

Baar

et al. 2020

Comp Rev Food Sci Food Safe

116

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Innovation in cultivated meat development has been rapidly accelerating in recent years because it holds the potential to help attenuate issues facing production of dietary protein for a growing world population. There are technical obstacles still hindering large‐scale commercialization of cultivated meat, of which many are related to the media that are used to culture the muscle, fat, and connective tissue cells. While animal cell culture media has been used and refined for roughly a century, it has not been specifically designed with the requirements of cultivated meat in mind. Perhaps the most common industrial use of animal cell culture is currently the production of therapeutic monoclonal antibodies, which sell for orders of magnitude more than meat. Successful production of cultivated meat requires media that is food grade with minimal cost, can regulate large‐scale cell proliferation and differentiation, has acceptable sensory qualities, and is animal ingredient‐free. Much insight into strategies for achieving media formulations with these qualities can be obtained from knowledge of conventional culture media applications and from the metabolic pathways involved in myogenesis and protein synthesis. In addition, application of principles used to optimize media for large‐scale microbial fermentation processes producing lower value commodity chemicals and food ingredients can also be instructive. As such, the present review shall provide an overview of the current understanding of cell culture media as it relates to cultivated meat.

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Screening of by-products from the food industry as growth promoting agents in serum-free media for skeletal muscle cell culture

Abstract: Hydrolysates from food by-products allow higher cell growth and metabolic activity than commercially available serum in skeletal muscle cell culture and can potentially be used to produce a tailor-made serum-free media.

Cited by 63 publications

References 39 publications

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Applications and analysis of hydrolysates in animal cell culture

Considerations for the development of cost‐effective cell culture media for cultivated meat production

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