Screening of Carbohydrates for Sporulation of Bacilli in Fluid Medium

Majumder, S. K.; Padma, M. C.

doi:10.1139/m57-069

Cited by 7 publications

(3 citation statements)

References 15 publications

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“…The reasons for the specificity of the effect of the L-pentoses on clostridial sporulation has not been previously reported. However, Majunder and Padma (10) observed a sporogenic effect of D-xylose in bacilli, but no stimulation with arabinose was noted. The probability of sporulation during growth was also dependent on the nature and concentration of the nitrogen sources.…”

Section: Discussionmentioning

confidence: 95%

Sporulation of the “Thermophilic Anaerobes.”

Pheil

Ordal

1967

Applied Microbiology

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A reasonable degree of synchrony in the sporulation of Clostridium thermosaccharolyticum 3814 was obtained by using three 10% transfers of 8-hr cultures in a medium containing 0.5% L-arabinose, 0.5% peptone, 0.5% yeast extract, and Gc minerals. Sporulation was stimulated by L-arabinose and L-xylose, but was repressed by glucose, mannose, fructose, and D-pentoses. Sporulating cells were long and thin, whereas repressed cells were shorter and thicker. The optimal pH for sporulation was in the range of pH 5.0 to 5.5. As sporulation continued, the accumulated acetate decreased. Label studies indicated that a significant amount of acetate-2-C'4 was incorporated into the spore lipid. The calcium, phosphorus, and dipicolinic acid (DPA) concentrations on a dry weight basis were 2.55, 2.60, and 7.25%, respectively. The molar ratio of Ca-DPA was 1.47.

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Section: Discussionmentioning

confidence: 95%

Sporulation of the “Thermophilic Anaerobes.”

Pheil

Ordal

1967

Applied Microbiology

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“…[Cr20 min exa few hours in broth cultures (11). This difference )r 20 mm in is probably due to the slow diffusion of the energy th medium source to the cells on the agar.…”

Section: (29%)mentioning

confidence: 99%

“…5 hr as com-It is known that some species of the Bacillaceae ate. After 3 sporulate in the presence of high levels of certain ibout 1.1% energy sources (5,11). Hsu and Ordal (5,6) of colony-demonstrated that Clostridium thermosaccharolytin the con-icum sporulated well when carbohydrates were in the pyru-present which limited the growth rate or when in the pyruglucose was fed to the culture at growth-limiting rates.…”

Section: (29%)mentioning

confidence: 99%

Physiological Studies of an Oligosporogenous Strain of Bacillus popilliae1

Costilow¹,

Coulter²

1971

Applied Microbiology

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A relatively small but consistent increase in the frequency of spore formation by an oligosporogenous strain of Bacillus popilliae (NRRL B-2309M) was obtained by adding 0.1% sodium pyruvate to the sporulation medium. The frequency of spore formation was essentially the same when a low level of glucose, trehalose, or glucose-6-phosphate or a high level of a-methyl-D-mannoside was added as the carbon and energy source. Many other variations in the cultural medium and cultural conditions failed to enhance spore formation of 2309M, and no spores were found in four asporogenic strains under any of the conditions tried. There were no significant differences between the 2309M strain and three nonsporeforming cultures with respect to (i) the rate and extent of growth, (ii) the rates of glucose utilization, or (iii) volatile acid production and utilization. None of the cultures tested was found to produce detectable levels of extracellular protease or an antibiotic. The only consistent marker found associated with spore formation was the development of catalase activity, and this activity was stimulated by heating at 80 C for 10 min. This was not found unless morphological evidence of spore formation was observed. The germination of the spores formed by 2309M in vitro was stimulated by heat shock and by the addition of pyruvate to the germination medium. ' Journal article no. 5553, Michigan Agricultural Experiment Station.

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Spore formation and germination in Saccharomyces

Miller¹,

Hoffmann‐Ostenhof²

1964

J of Basic Microbiology

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Screening of Carbohydrates for Sporulation of Bacilli in Fluid Medium

Cited by 7 publications

References 15 publications

Sporulation of the “Thermophilic Anaerobes.”

Sporulation of the “Thermophilic Anaerobes.”

Physiological Studies of an Oligosporogenous Strain of Bacillus popilliae1

Spore formation and germination in Saccharomyces

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