Screening of carotenoid-producing <i>Rhodotorula</i> strains isolated from natural sources

Tkáčová, Jana; Furdíková, Katarína; Klempová, Tatiana; Ďurčanská, Katarína; Čertı́k, Milan

doi:10.1515/acs-2015-0007

Cited by 13 publications

(8 citation statements)

References 21 publications

(22 reference statements)

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“…A red yeast Rhodotorula sp. KF-104 of plant origin (isolated from vegetative parts of vine) was found to produce a mixture of carotenoids, including β-carotene, γ-carotene, torulene and torularhodin, respectively [25].…”

Section: Exploration Of Native Carotenoid-producing Strainsmentioning

confidence: 99%

Biosynthetic Pathway of Carotenoids in Rhodotorula and Strategies for Enhanced Their Production

Tang¹,

Wang²,

Jun³

et al. 2019

Journal of Microbiology and Biotechnology

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Rhodotorula is a group of pigment-producing yeasts well known for its intracellular biosynthesis of carotenoids such as β-carotene, γ-carotene, torulene and torularhodin. The great potential of carotenoids in applications in food and feed as well as in health products and cosmetics has generated a market value expected to reach over $2.0 billion by 2022. Due to growing public concern over food safety, the demand for natural carotenoids is rising, and this trend significantly encourages the use of microbial fermentation for natural carotenoid production. This review covers the biological properties of carotenoids and the most recent findings on the carotenoid biosynthetic pathway, as well as strategies for the metabolic engineering methods for the enhancement of carotenoid production by Rhodotorula. The practical approaches to improving carotenoid yields, which have been facilitated by advancements in strain work as well as the optimization of media and fermentation conditions, were summarized respectively.

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Section: Exploration Of Native Carotenoid-producing Strainsmentioning

confidence: 99%

Biosynthetic Pathway of Carotenoids in Rhodotorula and Strategies for Enhanced Their Production

Tang¹,

Wang²,

Jun³

et al. 2019

Journal of Microbiology and Biotechnology

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“…Other carotenoids in the pigment extract could be torulene and torularhodin (according to their retention time in the HPLC analysis). Lycopene is a precursor and branch point for the synthesis of β‐carotene and torulene (Tkačova et al, ). The content of β‐carotene in the biomass of the marine Rhodotorula cultivated with the seaweed extract was very similar to that reported for Rhodotorula sp.…”

Section: Discussionmentioning

confidence: 99%

“…The content of β‐carotene in the biomass of the marine Rhodotorula cultivated with the seaweed extract was very similar to that reported for Rhodotorula sp. KF‐104 (39%) (Tkačova et al, ). Although the metabolic pathway for astaxanthin in Rhodotorula species has been described (Hernández‐Almanza et al ) no reports were found in literature demonstrating the synthesis of astaxanthin.…”

Section: Discussionmentioning

confidence: 99%

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Macrocystis pyriferasource of nutrients for the production of carotenoids by a marine yeastRhodotorula mucilaginosa

et al. 2019

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Aims To evaluate an aqueous extract of Macrocystis pyrifera as a nutrient source for the production of carotenoids by a marine Rhodotorula mucilaginosa isolated from seaweed samples. Materials and Results The effect of different culture conditions on the concentration of biomass and total pigments was evaluated using a Box–Behnken experimental design. The seaweed extract contained 15% w w−1 of protein and 20% w w−1 of carbohydrate; the main sugar in this fraction was trehalose (78%). The culture conditions that maximize the total pigment concentration (1·84 ± 0·03 mg l−1) were initial pH equal to 7, yeast extract as nitrogen source at a concentration of 4 g l−1, seaweed extract concentration at 25% v v−1, incubation performed at 25°C and 150 rev min−1 during 6 days. Under optimal growth conditions, three carotenoids were identified among the pigments produced by R. mucilaginosa, lycopene (38·4 ± 9·4%), β‐carotene (21·8 ± 1·5%) and astaxanthin (1·8 ± 0·3%). Conclusions Carotenoids of commercial interest (lycopene, β‐carotene and astaxanthin) can be produced using a marine R. mucilaginosa cultivated with an aqueous extract of M. pyrifera as nutrient source. The total pigment concentration in the culture ranged between 0·82 and 1·84 mg l−1, and was significantly affected by the concentration of the seaweed extract, and yeast extract. Significance and Impact of the Study This work demonstrates that M. pyrifera can be used as a nutrient source for the production of carotenoids by the marine yeast.

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“…Thus, the production of by bioprocesses involving microorganisms whose growth speed is relatively high, can ensure a productivity to the process which makes it advantageous [13,14].…”

Section: Introductionmentioning

confidence: 99%

Production of β-Carotene by a Newly Isolated Rhodotorula Glutinis UCP1555 Strain and Cytotoxic Effect Evaluation

Andrade¹,

Lima²,

Ribeaux³

et al. 2016

JCCE

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Abstract:Carotenoids have attracted greater attention due to the beneficial role on human health. It is an essential nutrient and has some biological functions such as species-specific coloration, photoprotection, light absorbing, which is an important component because of its role as precursor of vitamin A. In this study was performed the production of β-Carotene by Rhodotorula glutinis UCP/WFCC 1555 in presence and absence of blue and white using LED (light-emitting diodes) and evaluation of the cytotoxic effect. The production was investigated in low cost medium constituted by different concentrations of CG (crude glycerin) and CSL (corn steep liquor) from the CCD (Central Composite Design) and the identification and yield of the β-Carotene was investigated by chromatographic profile (HPLC). Additionally, the β-Carotene produced was tested to evaluate its cytotoxic effect in human tumor cells MCF-7 (breast cancer) and HL-60 (promyelocytic leukemia) and healthy cells of macrophages. The results showed that in the medium composed by 6% glycerin and 0.6% corn steep liquor, in the absence of light, occurred the maximum production of total carotenoids with values of 160 μg·g -1 and these 100.60 μg·g -1 correspond to the β-Carotene that showed ability in inhibit cell growth in several tumor cells such as MCF-7 cells (breast cancer) and HL-60 (promyelocytic leukemia).

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Screening of carotenoid-producing Rhodotorula strains isolated from natural sources

Cited by 13 publications

References 21 publications

Biosynthetic Pathway of Carotenoids in Rhodotorula and Strategies for Enhanced Their Production

Biosynthetic Pathway of Carotenoids in Rhodotorula and Strategies for Enhanced Their Production

Macrocystis pyriferasource of nutrients for the production of carotenoids by a marine yeastRhodotorula mucilaginosa

Production of β-Carotene by a Newly Isolated Rhodotorula Glutinis UCP1555 Strain and Cytotoxic Effect Evaluation

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