Screening of chemiluminescence constituents of cereals and DPPH radical scavenging activity of γ‐oryzanol

Abstract

The chemiluminescence (CL) constituents of cereals were detected by CL using the H(2)O(2)-acetaldehyde system. The cereals tested, such as rice, millet and sorghum, exhibited various levels of CL activity. The gamma-oryzanol fraction was extracted from brown rice and separated into four constituents by HPLC. The four constituents were identified as cycloartenyl ferulate, 24-methylenecycroartanyl ferulate, campesteryl ferulate and beta-sitosteryl ferulate. Free radical scavenging activities with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and CL intensities of four constituents (gamma-oryzanol components) were measured and compared with that of gallic acid, which is a typical free radical scavenger. Four constituents scavenged DPPH radicals and scavenging activities were proportional to CL intensities. Concentrations of four CL constituents required to quench 50% (IC(50)) of the free radicals ranged from 0.9 to 1.1 mmol/L. We demonstrated that measurement of CL intensities was a rapid and convenient method for screening DPPH radical scavenging activities of rice.

Antioxidant Activity of Individual Steryl Ferulates from Various Cereal Grain Sources

“…Akiyama et al reported similar DPPH • scavenging activity for SFs 1, 2, 5, and 6 and compound 9 (>100 μM). 20 Islam et al, who used a comparable reaction system with ours, also found SFs 1, 2, and 6 and compound 9 had similar DPPH • scavenging activities. 21 In another study, Kikuzaki et al reported that SF 1, SF 2, and ORY (20 μM) had the same DPPH • scavenging capacity, while compound 9 was slightly better than the SFs.…”

“…The DPPH • scavenging activities of SFs have also been reported in previous studies. Akiyama et al reported similar DPPH • scavenging activity for SFs 1 , 2 , 5 , and 6 and compound 9 (>100 μM) . Islam et al., who used a comparable reaction system with ours, also found SFs 1 , 2 , and 6 and compound 9 had similar DPPH • scavenging activities .…”

“…24 Nevertheless, some observations also showed similar antioxidant activity for individual SFs in DPPH. 20,21 In this study, generally, the difference in antioxidant activity of individual SFs was very small and depended greatly on the reaction models. In addition to the inner-molecular variations, the intermolecular interactions, as well as interactions with the solvent (environment), should also be considered.…”

Antioxidant activity of phytosteryl phenolates

“…The maintaining or enhancing of the antiradical capacity of phytosteryl phenolates suggests that these derivatives may be used as antioxidants in more lipophilic systems. As one of the main constituents of γ ‐oryzanol, phytosteryl ferulates, including sitosteryl ferulate, and campesteryl ferulate, have been reported to possess high free radical scavenging capacity . In our study, phytosteryl caffeates are the only group of compounds that showed higher radical scavenging capacity than the reference trolox.…”

Dietary Oryzanol (Ory) Improved the Survival and Growth of Large Yellow Croaker (Larimichthys crocea) Larvae via Promoting Activities of Digestive Enzymes, Antioxidant Capacity, and Lipid Metabolism

“…Previous research in mammals and livestock has highlighted the beneficial role of Ory in terms of survival and growth [10][11][12]. Besides, Akiyama et al [13] reported Ory's high radical scavenging capacity in mammalian systems. Moreover, studies by Wang et al [10] and Minatel et al [14] demonstrated the Ory is associated with antiinflammatory properties and cholesterol-lowering effects in mammals and livestock, respectively.…”