Screening of colistin-resistant bacteria in livestock animals from France

Abstract

Colistin is frequently used as a growth factor or treatment against infectious bacterial diseases in animals. The Veterinary Division of the European Medicines Agency (EMA) restricted colistin use as a second-line treatment to reduce colistin resistance. In 2020, 282 faecal samples were collected from chickens, cattle, sheep, goats, and pigs in the south of France. In order to track the emergence of mobilized colistin resistant (mcr) genes in pigs, 111 samples were re-collected in 2021 and included pig faeces, food, and water from the same location. All samples were cultured in a selective Lucie Bardet Jean-Marc Rolain (LBJMR) medium and colonies were identified using MALDI-TOF mass spectrometry and then antibiotic susceptibility tests were performed. PCR and Sanger sequencing were performed to screen for the presence of mcr genes. The selective culture revealed the presence of 397 bacteria corresponding to 35 different bacterial species including Gram-negative and Gram-positive. Pigs had the highest prevalence of colistin-resistant bacteria with an abundance of intrinsically colistin-resistant bacteria and from these samples one strain harbouring both mcr-1 and mcr-3 has been isolated. The second collection allowed us to identify 304 bacteria and revealed the spread of mcr-1 and mcr-3 in pigs. In the other samples, naturally, colistin-resistant bacteria were more frequent, nevertheless the mcr-1 variant was the most abundant gene found in chicken, sheep, and goat samples and one cattle sample was positive for the mcr-3 gene. Animals are potential reservoir of colistin-resistant bacteria which varies from one animal to another. Interventions and alternative options are required to reduce the emergence of colistin resistance and to avoid zoonotic transmissions.

Repeated Occurrence of Mobile Colistin Resistance Gene-Carrying Plasmids in Pathogenic Escherichia coli from German Pig Farms

“…The global dissemination of colistin resistance genes in bacteria from humans, animals and the environment has been reported over the last years [24,44]. The occurrence of mcr genes has been investigated in various studies, especially those conducted concerning pig and poultry production [45][46][47]. However, data on recurrent mcr-mediated resistance in livestock farms are scarce [48,49].…”

Towards identification of new genetic determinants for post‐weaning diarrhea in piglets

“…Strains of pathogens, resistant to antibiotics, are a serious peril not only to the animal production industry but also to human health as an increasing number and variety of antibiotic resistant bacteria are found in pigs and other production animals (Hamame et al, 2022;Li et al, 2019Li et al, , 2023. Therefore, gaining a deeper understanding of the pathological mechanisms underlying PWD is important in order to develop new, sustainable preventive measures.…”

Complete Genome Sequence of Colistin-Resistant Salmonella enterica Serotype Enteritidis Strain CRSE-01 Isolated from Poultry Carcass in South Korea

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Colistin has been used for last-line treatment of multidrug-resistant (MDR) Gram-negative bacterial infection [1]. Recently, the colistin-resistant Salmonella enterica isolates have been found globally, emerging as serious threats to human and veterinary health [2]. In South Korea, S. enterica serotype Enteritidis (S. Enteritidis) has been one of the dominant serotypes involved in Salmonella outbreaks [3].

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