Screening of Different Probiotic Strains for Their In Vitro Ability to Metabolise Oxalates

Mogna, Luca; Pane, Marco; Nicola, Stefania; Raiteri, Elisabetta

doi:10.1097/mcg.0000000000000228

Cited by 58 publications

(39 citation statements)

References 37 publications

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“…Although there are huge individual differences, oxalic acid-degrading bacteria are present in the endogenous microbiota of the human intestine. Recently, Mogna et al [23] demonstrated the potential therapeutic application of some probiotics, mainly Lactobacillus and Bifidobacterium species, in order to reduce hyperoxaluria in vitro using reversed-phase high-performance liquid chromatography. In this study, researchers tested the oxalate-degrading activity of 13 lactobacilli and 5 bifidobacteria, and O. formigenes was used as the positive reference to validate the capability of high-performance liquid chromatography to assess oxalate degradation.…”

Section: Discussionmentioning

confidence: 99%

The Relationship between Serum Oxalic Acid, Central Hemodynamic Parameters and Colonization by Oxalobacter formigenes in Hemodialysis Patients

et al. 2015

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Background/Objective: Elevated pulse wave velocity (PWV) and central aortic blood pressures are independent predictors of increased cardiovascular morbidity and mortality in hemodialysis (HD) patients. Oxalic acid is a uremic retention molecule that is extensively studied in the pathogenesis of calcium oxalate stones. Oxalobacter formigenes, a member of the colon microbiota, has important roles in oxalate homeostasis. Data regarding the colonization by and the exact role of O. formigenes in the pathogenesis of oxalic acid metabolism in HD patients are scant. Hence, we aimed to determine the relationship between fecal O. formigenes colonization, serum oxalic acid and hemodynamic parameters in HD patients with regard to the colo-reno-cardiac axis. Methods: Fifty HD patients were enrolled in this study. PWV and central aortic systolic (cASBP) and diastolic blood pressures (cADBP) were measured with a Mobil-O-Graph (I.E.M. GmbH, Stolberg, Germany). Serum oxalic acid levels were assessed by ELISA, and fecal O. formigenes DNA levels were isolated and measured by real-time PCR. Results: Isolation of fecal O. formigenes was found in only 2 HD patients. One of them had 113,609 copies/ml, the other one had 1,056 copies/ml. Serum oxalic acid levels were found to be positively correlated with PWV (r = 0.29, p = 0.03), cASBP (r = 0.33, p = 0.001) and cADBP (r = 0.42, p = 0.002) and negatively correlated with LDL (r = -0.30, p = 0.03). In multivariate linear regression analysis, PWV was independently predicted by oxalic acid, glucose and triglyceride. Conclusions: This is the first study that demonstrates the absence of O. formigenes as well as a relation between serum oxalic acid and cASBP, cADBP and PWV in HD patients. Replacement of O. formigenes with pre- and probiotics might decrease serum oxalic acid levels and improve cardiovascular outcomes in HD patients.

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Section: Discussionmentioning

confidence: 99%

The Relationship between Serum Oxalic Acid, Central Hemodynamic Parameters and Colonization by Oxalobacter formigenes in Hemodialysis Patients

et al. 2015

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“…The human body does not produce the enzymes needed to directly metabolize oxalate in the digestive tract. Oxalates are metabolized by the intestinal microflora …”

Section: In Vitro Studiesmentioning

confidence: 99%

“…The intestinal microflora, probiotics and prebiotics significantly influence the bioavailability of minerals. The intestinal microbiota, probiotics and prebiotics can increase the absorption of minerals, especially iron and calcium, or decrease it …”

Section: Introductionmentioning

confidence: 99%

Association between the gut microbiota and mineral metabolism

2017

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The aim of this review is to present the most recent scientific evidence of interactions between the intestinal microbiota and minerals, and the effect of this interaction on the health of the host. The Web of Science database from the years 2013-2017 on this topic was reviewed. Numerous in vitro studies have shown that iron significantly affects the intestinal microbiota. However, Bifidobacteriaceae are capable of binding iron in the large intestine, thereby limiting the formation of free radicals synthesized in the presence of iron, and thus reducing the risk of colorectal cancer. Animal studies have revealed that supplementation with probiotics, prebiotics and synbiotics has a significant effect on bone calcium, phosphate and bone metabolism. The dynamic interaction between microbiota and zinc was shown. Human studies have provided evidence of the influence of probiotic bacteria on parathormone, calcium and phosphate levels and thus on bone resorption. Recent studies have produced new information mainly on the impact of the intestinal bacteria on the metabolism of calcium and iron. From a scientific perspective, the most urgent fields that remain to be investigated are the identification of all human gut microbes and new therapies targeting the interaction between intestinal bacteria and minerals. © 2017 Society of Chemical Industry.

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“…For example, under controlled conditions O. formigenes degraded up to 98% of available oxalate (37). On the other hand, in the same studies Lactobacillus and Bifidobacterium spp.…”

Section: Other Oxalate Degradersmentioning

confidence: 94%

Probiotics for prevention of urinary stones

Lieske

2017

Ann. Transl. Med.

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Background: Urinary supersaturation is one key determinant of calcium oxalate (CaOx) urinary stone formation, and urinary excretions of oxalate and citrate are two key determinants. Each is influenced by gastrointestinal processes.Methods: Open label and randomized placebo studies have examined the effect of oral probiotic preparations on urinary supersaturation and oxalate excretion. Cross sectional studies in humans have studied the association of Oxalobacter formigenes colonization status and urinary oxalate excretion and prevalence of urinary stones. The intestinal microbiome of representative animals adapted to a high oxalate diet has been defined. Results:The fecal content of O. formigenes, the best studied oxalate-degrader, varies depending on stone status.However, trials with probiotics designed to degrade oxalate including those containing O. formigenes, Lactobacillus, and/or Bifidobacterium spp., have been disappointing. Multiple intestinal segments of animals on a high oxalate diet contains diverse communities of microorganisms that can function together to degrade and detoxify a large oxalate load.Conclusions: Although the intestinal microbiome seems likely to play a role to modify gastrointestinal absorption of lithogenic substances and hence urinary stone risk, whether we can develop tools to manipulate it and decrease this kidney stone risk remains to be determined.

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Screening of Different Probiotic Strains for Their In Vitro Ability to Metabolise Oxalates

Cited by 58 publications

References 37 publications

The Relationship between Serum Oxalic Acid, Central Hemodynamic Parameters and Colonization by <b><i>Oxalobacter formigenes </i></b>in Hemodialysis Patients

The Relationship between Serum Oxalic Acid, Central Hemodynamic Parameters and Colonization by <b><i>Oxalobacter formigenes </i></b>in Hemodialysis Patients

Association between the gut microbiota and mineral metabolism

Probiotics for prevention of urinary stones

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