Stem and root rot disease caused by Macrophomina phaseolina is the serious soil-borne disease in sesame. Thepresent study was conducted at College of Agriculture, Jodhpur during kharif season 2020-21 to evaluate the nine different fungicides under in vitro condition and five different fungicides under in vivo against M. phaseolina. Result of in vitro study indicated that the fungicide, carbendazim (50% WP) gave complete mycelial growth inhibition (100%) at 500 and 1000 ppm concentrations, respectively. Second best of pyraclostrobin 5% + metiram 55% WG, carbendazim12% + mancozeb 63%, then propiconazole 25% EC, thiophanate methyl (70% WP) and cymoxanil 8% + mancozeb 64% also gave 100% inhibition of mycelial growth at 1000 ppm respectively. The lowest disease per cent was recorded in combination fungicide of tebuconazole 50% + trifloxystrobin 25% WG (8.91%) followed by 9.14% with cymoxanil 8% + mancozeb 64%, pyraclostrobin 5% + metiram 55% WG (11.02%) whereas, maximum disease incidence was recorded in control plot (55.32%) when tested under in vivo condition.