Screening of endophytic fungi stored in a culture collection for taxol production

Andrade, Hortência Farias de; Araújo, Lívia Caroline Alexandre de; Santos, Bruno Souza dos; Paiva, Patrícia Maria Guedes; Napoleão, Thiago Henrique; Correia, Maria Tereza dos Santos; Oliveira, Maria Betânia Melo de; Lima, Gláucia Manoella de Souza; Ximenes, Rafael Matos; Silva, Túlio Diego da; Silva, Girliane Regina da; Silva, Márcia Vanusa da

doi:10.1016/j.bjm.2018.06.001

Cited by 17 publications

(9 citation statements)

References 18 publications

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“…The endophytic production of secondary metabolites varies according to the biology and growth conditions of the microorganism and are produced as an adaptation to specific functions in nature. They play vital roles in vivo owing to the numerous metabolic interactions observed between endophytes and their hosts, such as signaling, defense, and regulation of symbiosis (De Andrade et al 2018). In view of this, the search for new bioactive molecules should focus on organisms that inhabit new ecosystems or niches that are still unexplored, because natural products are adapted to a specific function in nature (Azevedo et al 2000, Nisa et al 2015.…”

Section: Discussionmentioning

confidence: 99%

Chemical, diversity and biotechnological potential of endophytic fungi isolated from Brazilian Cerrado plants

2021

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This is a systematic review of scientific articles reporting the biodiversity of endophytic fungi isolated from endemic Cerrado plants in Brazil and exposes the potential applications of these microorganisms in the production of known and new metabolites. The “Portal de Periódicos” (CAPES), Science Direct, PubMed, Scielo, and Biblioteca Virtual em Saúde (BVS) databases were used. A total of 418 articles were found using the following sets of keywords: Endophytic, fungi, and Cerrado; endophytic, fungi, rupestrian, and grass lands; endophytic, fungi, Brazilian, and savanna; among these, 14 articles were selected. The articles were grouped into studies on the biodiversity of Cerrado endophytic fungi, chemical studies, and other subjects. All the articles were found to be related to the diversity present in the Cerrado of the southeastern and midwestern regions, they show high fungal biodiversity with Diaporthe genus predominant in almost all plants studied. In all the studies on the chemical diversity of endophytic fungi, new compounds or new natural products were found associated with a range of bioactivity, especially antifungal. It shows the great potential of endophytes in the production of new and bioactive secondary metabolites, as well as the unexplored chemical diversity of endophytes isolated from endemic plant species. The review shows that rare studies performed in regions where the Cerrado biome are predominant, including the central, north, and northeast regions, representing an important niche that is still unexplored.

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Section: Discussionmentioning

confidence: 99%

Chemical, diversity and biotechnological potential of endophytic fungi isolated from Brazilian Cerrado plants

2021

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“…These genes encode the enzymes 10-deacetylbaccatin III-10-O-acetyl-transferase and C-13 phenylpropanoid side-chain-CoA acyltransferase, respectively, that are involved in the Taxol biosynthetic pathway of Taxus spp. Although these PCR primers have been used to screen gDNA from fungi deemed able to synthesize the compound [36-38], the reverse PCR primer designed by Zhang and colleagues [39] for the dbat gene, is based on the plant sequence (GenBank No. EF028093) and is an intron-spanning primer.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, another dbat sequence for the basidiomycete-fungus, Grammothele lineata , published by Das, Rahman, et al, 2017 [42], does not seem to be present in the draft genome of the fungus, published later that year [43]. While in some reports the dbat gene has been detected, their sequences were thought to be non-specific amplification and BLASTn searches produced no significant hits, or simply the dbat sequences were not published in GeneBank [36-38]. Thus, our negative results obtained during the initial PCR screening using the published primers for dbat and bapt would be consistent with those obtained for published fungal genomes, that is, genes with significant homology to some of the plant genes involved in Taxol biosynthesis, are absent [40, 44].…”

Section: Discussionmentioning

confidence: 99%

Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

Gauchan

Vélëz

Acharya

et al. 2020

Preprint

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24Endophytes are microbial colonizers that reside in plants by symbiotic association produces 25 several biological classes of natural products. The current study focuses on the isolation and 26 characterization of bioactive compounds produced by endophytic fungi isolated from the 27 Himalayan yew (Taxus wallichiana) collected from the Mustang district of Nepal. The plant 28 materials were collected from the Lower-Mustang region in the month of October 2016 and the 29 endophytic fungi were isolated on artificial media from inner tissues of bark and needles. 30 Antimicrobial and antioxidant activity, along with total phenolic-and flavonoid-content assays, 31 were used in the evaluation of bioactivity of the fermented crude extracts along with the in vitro 32 ability of the endophytes to produce the anticancer compound Taxol was analyzed. A total of 16 33 fungal morphotypes were obtained from asymptomatic inner tissues of the bark and needles of T. 34 wallichiana. Among the 16 isolates, the ethyl acetate (EA) fraction of isolate MUS1, showed 35 antibacterial and antifungal activity against all test-pathogens used, with significant inhibition 36 against Pseudomonas aeruginosa ATCC 27853 (MIC: 250 µg/ml) and the pathogenic yeast, 37 Candida albicans (MIC: 125 µg/ml). Antioxidant activity was also evaluated using 2,2-diphenyl-38 1-picrylhydrazyl (DPPH). At a concentration of 100 µg/ml, the % radical scavenging activity 39 was 83.15±0.40, 81.62±0.11, and 62.36±0.29, for ascorbic acid, butylated hydroxytoluene (BHT) 40 and the EA fraction of MUS1, respectively. The DPPH-IC50 value for the EA fraction was 81.52 41 µg/ml, compared to BHT (62.87 µg/ml) and ascorbic acid (56.15 µg/ml). The total phenolic and 42 flavonoid content in the EA fraction were 16.90±0.075 µg gallic acid equivalent (GAE) and 43 11.59±0.148 µg rutin equivalent (RE), per mg of dry crude extract, respectively. Isolate MUS1, 44 identified as an Annulohypoxylon sp. by ITS sequencing, also produced Taxol (282.05 µg/L) as 45 shown by TLC and HPLC analysis. Having the ability to produce antimicrobial and antioxidant 46 3 compounds, as well as the anticancer compound Taxol, makes Annulohypoxylon sp. strain 47 MUS1, a promising candidate for further study given that naturally occurring bioactive 48 compounds are of great interest to the pharmacological, food and cosmetic industries.49

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“… South Korea [ 170 ] Michelia champaca L Needles Dichloromethane HPLC, UV Chaetomium sp. India [ 186 ] Moringa oleifera Lam Leaves Dichloromethane LC–MS, IR, NMR, UV, HPLC Cladosporium oxysporum India [ 152 ] Morinda citrifolia L Leaves Dichloromethane HPLC, NMR, UV, IR, FAB-MS Lasiodiplodia theobromae India [ 148 ] Plantago major L Leaves Ethyl acetate extract LC–MS, UV Nigrospora sphaerica India [ 187 ] Colletotrichum gloesporioides Alternaria alternata Glomerella cingulate Salacia oblonga Wall Bark Genomic mining Armilaria sp. India [ 188 ] …”

Section: Ptx Derived From Endophytes Of Different Plant Species For I...mentioning

confidence: 99%

Paclitaxel and its semi-synthetic derivatives: comprehensive insights into chemical structure, mechanisms of action, and anticancer properties

Sati,

Sharma,

Dhyani

et al. 2024

Eur J Med Res

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Cancer is a disease that can cause abnormal cell growth and can spread throughout the body. It is among the most significant causes of death worldwide, resulting in approx. 10 million deaths annually. Many synthetic anticancer drugs are available, but they often come with side effects and can interact negatively with other medications. Additionally, many chemotherapy drugs used for cancer treatment can develop resistance and harm normal cells, leading to dose-limiting side effects. As a result, finding effective cancer treatments and developing new drugs remains a significant challenge. However, plants are a potent source of natural products with the potential for cancer treatment. These biologically active compounds may be the basis for enhanced or less toxic derivatives. Herbal medicines/phytomedicines, or plant-based drugs, are becoming more popular in treating complicated diseases like cancer due to their effectiveness and are a particularly attractive option due to their affordability, availability, and lack of serious side effects. They have broad applicability and therapeutic efficacy, which has spurred scientific research into their potential as anticancer agents. This review focuses on Paclitaxel (PTX), a plant-based drug derived from Taxus sp., and its ability to treat specific tumors. PTX and its derivatives are effective against various cancer cell lines. Researchers can use this detailed information to develop effective and affordable treatments for cancer.

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Screening of endophytic fungi stored in a culture collection for taxol production

Cited by 17 publications

References 18 publications

Chemical, diversity and biotechnological potential of endophytic fungi isolated from Brazilian Cerrado plants

Chemical, diversity and biotechnological potential of endophytic fungi isolated from Brazilian Cerrado plants

Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

Paclitaxel and its semi-synthetic derivatives: comprehensive insights into chemical structure, mechanisms of action, and anticancer properties

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