Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae

Li, Shan; Luo, Shuangshuang; Yin, Xinran; Zhao, Xingying; Wang, Xuyang; Gao, Song; Xu, Sha; Lu, Jian; Zhou, Jingwen

doi:10.1016/j.synbio.2024.06.005

Synthetic and Systems Biotechnology

2024

DOI: 10.1016/j.synbio.2024.06.005

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Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae

Shan Li,

Shuangshuang Luo,

Xinran Yin

et al.

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2024

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Yeast Synthesis and Herbicidal Activity Evaluation of Aspterric Acid

Zhou,

Zhang,

et al. 2024

J. Agric. Food Chem.

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Aspterric acid (AA) is a novel natural product herbicide that targets dihydroxyacid dehydratase in plants. In this study, we introduced two distinct AA biosynthesis-related gene clusters into Saccharomyces cerevisiae and screened the core biosynthetic enzyme, sesquiterpene cyclase, from various fungi. The combination of sesquiterpene cyclase from Aspergillus taichungensis IBT 19404 and two cytochrome P450s from Penicillium brasilianum resulted in the optimal AA synthesis efficiency in yeast, with the highest titer of 33.21 mg/L achieved by optimizing fermentation conditions in shake flasks. Moreover, the herbicidal effects of AA on weed germination and growth were evaluated. Notably, AA strongly inhibited the germination of Amaranthus tricolor, Portulaca oleracea, Bidens pilosa, Lolium perenne, and Leptochloa chinensis. Furthermore, AA could also inhibit the shoot and root growth of weeds with a superior inhibitory effect on roots relative to shoots. Our work not only provides a sustainable method for the biosynthesis of AA in yeast but also paves the way for the application of AA as a preemergence herbicide.

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Yeast Synthesis and Herbicidal Activity Evaluation of Aspterric Acid

Zhou,

Zhang,

et al. 2024

J. Agric. Food Chem.

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Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae

Cited by 1 publication

References 48 publications

Yeast Synthesis and Herbicidal Activity Evaluation of Aspterric Acid

Yeast Synthesis and Herbicidal Activity Evaluation of Aspterric Acid

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