Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol.

Abstract

Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.

A comparative and functional genomics analysis of the genusRomboutsiaprovides insight into adaptation to an intestinal lifestyle

“…This suggests that these strains are able to ferment glycerol and produce 1,3-propanediol as one of the fermentation end-products. Production of 1,3-propanediol has indeed been reported for R. lituseburensis (22). Furthermore, in the genome of R. lituseburensis A25K T the oxidative pathway for glycerol metabolism, including glycerol dehydrogenase and dihydroxyacetone kinase, could be identified as well, suggesting that this strain should be able to use glycerol as sole carbon and energy source.…”

“…The type strain of the second validly described species within the genus Romboutsia, R. lituseburensis A25K T , is not of intestinal origin, but was originally isolated from soil and humus from Côte d’Ivoire (20). Based on 16S rRNA gene identity, strains very similar to R. lituseburensis A25K T have been isolated in recent years: strain H17 was isolated from the main anaerobic digester of a biogas plant (GI: EU887828.1), strain VKM B-2279 was isolated from a p-toluene sulfonate degrading community (21), strain 2ER371.1 was isolated from waste of biogas plants (22), and strain E2 was isolated from a cellulose degrading community enriched from mangrove soil (23). Furthermore, the type strain of the recently described novel species R. sedimentorum has been isolated from sediment samples taken from an alkaline-saline lake located in Daqing oilfield.…”

1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5.

“…For comparision, a single-stage culture of C. butyricum with raw glycerol produced up to 35-48 g of 1,3-PDO per litre with a yield of 0.55 g per one gram of glycerol (Papanikolaou et al, 2000;Chatzifragkou et al, 2011). Glycerol fermentation by the C. butyricum 2CR371.5, which was the source of the genes studied here, results in 0.57 g of 1,3-PDO per 1 g of glycerol consumed (Dąbrowski et al, 2012).…”

“…Here we report construction of a recombinant E. coli strain producing 1,3-PDO from glycerol by introducing dhaB1, dhaB2, and dhaT genes from the dha operon from C. butyricum 2CR371.5, a strain from our collection of environmental samples and strain. The strain was previously isolated and tested as a 1,3-PDO producer and the results were described in the accompanying article (Dąbrowski et. al, 2012).…”

Effect of crude and pure glycerol on biomass production and trehalose accumulation by Propionibacterium freudenreichii ssp. shermanii 1

“…One of them, crude glycerol, is a principal waste of biodiesel production. It is estimated that the manufacture of biodiesel generates about 10% crude glycerol (Santibáñez et al, 2011;Dąbrowski et al, 2012;Yang et al, 2012). As predicted, biodiesel production has been increasing.…”