Screening of Estrogenic-Disrupting Compounds in Dairy Products Based on the Estrogen Receptor Cocktail

Abstract

The residue of estrogenic-disrupting compounds (EDCs) that are secreted by cows, added as drugs, and present in the feed may exist in dairy products. A gold nanoparticles (AuNPs)-estrogen receptor (ER) cocktail colorimetric assay equipped with ER cocktail solid phase extraction (SPE) was established to screen EDCs. Nine EDCs with high, moderate, and low estrogenic activity were selected to be the representative targets. The recognition range of the colorimetric assay combined with the ER cocktail SPE was wider than that of a single ERα or ERβ. The lowest detection limit of the established assay was about 10−9 mg·mL−1. The detection limits of estrone, bisphenol A, and bisphenol B were about one order of magnitude lower than the method based on a single ER. The recoveries of the spiked nine EDCs were between 80.0% and 110.0%, and daidzein was identified in the dairy product. The developed method has potential application prospects in food safety and environmental monitoring.

Advancements and greenification potential of magnetic molecularly imprinted polymers for chromatographic analysis of veterinary drug residues in milk

“…Metabolites persist in animals and are released into the environment as environmental estrogens through excretion. These enter the human food chain, and their residues in dairy products can disrupt human physiological functions, potentially leading to cancers and hormone synthesis disruptions (Li et al., 2022). It can also lead to vitellogenin overproduction and disrupt sexual development in aquatic organisms (Barreiros et al., 2016).…”