Screening of ESTs from Septoria musiva (teleomorph Mycosphaerella populorum) for detection of SSR and PCR–RFLP markers

Abstract

The development of an expressed sequence tag (EST) collection for the poplar pathogen Septoria musiva presented an opportunity for the development of locus‐specific polymorphic markers. Five microsatellites simple sequence repeat (SSR) and six polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) markers were identified and tested on three S. musiva populations collected in the north‐central and northeastern regions of North America. The results obtained support previously published random amplified polymorphic DNA (RAPD) data.

Recent advances related to poplar leaf spot and canker caused bySeptoria musiva

“…Recently, development of a large number of single nucleotide polymorphisms (SNPs) in fungal pathogens has allowed reliable and sensitive delimitation of individuals and the study of genetic differentiation and adaptation (Feau et al, 2006b(Feau et al, , 2007a. These studies reveal that populations of poplar pathogens have a strong capacity to adapt to sudden environmental changes, such as the advent of new genotypes of the host (Feau et al, 2005a(Feau et al, , 2006b(Feau et al, , 2007a.…”

Identification and genetic mapping of highly polymorphic microsatellite loci from an EST database of the septoria tritici blotch pathogen Mycosphaerella graminicola

“…Therefore, a great need exists for the identification and genetic mapping of additional microsatellite loci in these species. Presently, high-throughput identification of microsatellite sequences in databases of expressed sequence tag (EST) sequences and completed genomes provides a faster and easier method for identifying microsatellite loci (Breuillin et al, 2006;Feau et al, 2006;Hosid et al, 2005;Karaoglu et al, 2005;Lim et al, 2004;Sainudiin et al, 2004). Within EST sequences, microsatellite loci have been identified primarily in the 5 0 leader but also in coding regions and occasionally in introns of unprocessed or improperly spliced messenger RNAs (Kantety et al, 2002;La Rota et al, 2005;Serapion et al, 2004).…”

Development of genomic SSR markers and genetic diversity of Sphaerulina musiva in China

“…In previous studies, six RAPD markers, six RFLP markers and five EST-SSR markers were developed for S. musiva (Feau et al, 2005(Feau et al, , 2006. At present SSR markers are the most commonly used Due to lack of molecular markers, there are limited studies on genetic diversity of S. musiva.…”

“…In previous studies, six RAPD markers, six RFLP markers and five EST‐SSR markers were developed for S. musiva (Feau et al., 2005, 2006). At present SSR markers are the most commonly used molecular markers for genetic diversity and population structure analyses, as SSR markers have the advantages of codominance, selective neutrality, high information and repeatability (Chambers & MacAvoy, 2000; Ellegren, 2004).…”

“…For example, 12, 56 and 122 pairs of SSR primers were developed and used to analyse genetic diversity of Rhizoctonia cerealis , Puccinia graminis and Ustilago maydis , respectively (Karaoglu et al., 2013; Li et al., 2017; Zhang et al., 2015). However, only five EST‐SSR markers have been developed in S. musiva at present (Feau et al., 2006). Though six RAPD markers and six RFLP markers can also be used for S. musiva , these markers are not mainstream molecular markers.…”