Screening of extracellular polymeric substance producing bacteria isolated from environmental sources and testing their ability to seal fractures zones of oil reservoir rocks

Abstract

In this study, 21 environmental bacterial isolates isolated from different environmental sources in Basra Governorate identified phenotypically and genetically based on 16 rRNA gene sequence analysis were screened to reveal their ability to produce biopolymer based on the viscosity scale formed in the cultural media and use of Sudan Black stain. Six isolates showed their ability to produce biopolymer, and the two isolates of Bacillus licheniformis strain CC91, Bacillus thuringiensis strain LU3 recorded the best results. The isolate of the Bacillus licheniformis strain CC91 was tested in the process of sealing fractures and openings with high permeability for samples of oil reservoir rocks.

Isolation, screening, and characterization of polyhydroxyalkanoate (PHA)‐producing Cellulosimicrobium cellulans: A promising approach for eco‐friendly biopolymer production

“…BPPI‐14 and BPPI‐19 families, produced black grains when stained with Sudan Black 30 . According to the literature, Bacillus licheniformis strain CC91 and Bacillus thuringiensis strain LU3 oil reservoir rocks showed positive results for Sudan Black staining 31 . Sudan Black‐B has a significant affinity for lipid‐interacted biopolymers, which is important in the screening of PHA manufacturers.…”

“…30 According to the literature, Bacillus licheniformis strain CC91 and Bacillus thuringiensis strain LU3 oil reservoir rocks showed positive results for Sudan Black staining. 31 Sudan Black-B has a significant affinity for lipid-interacted biopolymers, which is important in the screening of PHA manufacturers. Additionally, the microscopic data partially demonstrated that the isolate's intracellular lipid and biopolymer concentration was connected to its cytoplasmic components and structural specifications.…”