Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

Abstract

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL -1 . The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.

Extracción y caracterización preliminar de pigmentos y colágeno a partir de medusas Stomolophys meleagris, Aurelia aurita, Rhizostoma pulmo y Rophilema esculentum

“…Molecular weight of the protein moiety. Carbohydrate and protein contents of the crude extract were 10% and 540 µg/mL, respectively, in agreement to data previously reported for Rhopilena esculentum [27] where the most abundant sugars are rhamnose, fucose, arabinose, mannose and N-acetyl galactosamine [22].…”