Screening of Feed Components for Salmonella with Polyvalent H. Agglutination

Barkate, John A.

doi:10.1128/aem.16.12.1872-1874.1968

Cited by 7 publications

(4 citation statements)

References 2 publications

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“…Membranes III and IV were developed in benzidine (-B-). Membranes I and III were spotted as follows: spots [1][2][3][4][5] contained 107, 106, 105, 104, and 103 S. typhimurium cells, respectively; spot 6 contained 107 Proteus sp. cells; spot 7 contained 107 E. coli cells.…”

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confidence: 99%

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Use of enzyme-labeled antibodies to detect Salmonella in foods

Krysinski¹,

Heimsch²

1977

Appl Environ Microbiol

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An indirect enzyme-labeled antibody technique (ELAT), in which Salmonella typhimurium was used as a model, was developed as a method to detect Salmonella in food samples. A cellulose-acetate membrane filter, the matrix for detection, was placed on a membrane-filter base and overlaid with a multiwelled lucite template. Mixed broth enrichment cultures were dispensed in the template wells, and cells were spotted onto the membrane via suction. After fixation, the membranes were immersed in rabbit anti-S. typhimurium flagella antibody, washed, immersed in goat anti-rabbit antibody conjugated to peroxidase, and washed. Exposure of membranes to the substrates 3,3'-diaminobenzidine or benzidine resulted in development of brown or blue macroscopic reaction products, respectively, on spots containing S. typhimurium. ELAT results agreed with those of enrichment serology and cultural procedures on three food products containing known levels ofS. typhimurium. Because of the magnification effect of the enzyme-substrate reaction, fewer cells were needed for detection than with enrichment serology, thereby reducing the total analysis time. The ability to test 14 or more samples simultaneously on a 47-mm membrane filter would facilitate screening large numbers of samples. Pending the development of a pure H antisera pool for the common Salmonella serotypes free from 0 antibodies, the ELAT demonstrated potential as a Salmonella detection methodology.

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confidence: 99%

“…Accelerated Salmonella detection methodologies have been developed to reduce the analysis time. Ofthese, fluorescent-antibody techniques (11,12,16) and enrichment serology (4,18) have been most popular. Fluorescent-antibody techniques are less reliable than cultural procedures, producing a high percentage of false-positive and false-negative results (7,13).…”

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confidence: 99%

Use of enzyme-labeled antibodies to detect Salmonella in foods

Krysinski¹,

Heimsch²

1977

Appl Environ Microbiol

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“…1991). One of the frequently cited origins of salmonellae in poultry is feed (Barkate 1968; Heres et al. 2004; Lofstrom et al.…”

Section: Introductionmentioning

confidence: 99%

“…Measures for the prevention and control of Salmonella throughout the poultry production chain are applied to raw materials, environmental sources and finished raw product (De Zutter et al 1991). One of the frequently cited origins of salmonellae in poultry is feed (Barkate 1968;Heres et al 2004;Lofstrom et al 2004;Franco 2005). Therefore, adequate control of Salmonella contamination of feed and colonization of chicks (caeca) is very important.…”

Section: Introductionmentioning

confidence: 99%

A validated miniaturized MPN method, based on ISO 6579:2002, for the enumeration of Salmonella from poultry matrices

Pavic

Groves

Bailey³

et al. 2010

Journal of Applied Microbiology

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Aims: To validate the effectiveness of a miniaturized most probable number method (mMPN) in enumerating Salmonella from poultry matrices. Methods and Results: A MPN was developed, based on the ISO 6579:2002 method using modified semi‐solid Rappaport–Vassiliadis media as the sole selective medium. The validation of the mMPN was shown to not differ significantly from, at the 95% confidence level (Student’s t‐test P = 0·357) to, the traditional 9‐tube MPN (tMPN) using pure cultures of Salmonella ser. Typhimurium, Infantis, Montevideo, Muenster and Salmonella subsp II 1,4,12,27:b:[e,n,x] (Sofia). The validation of naturally and artificially contaminated poultry matrices (carcasses, scald tank water, faeces, caeca and feed) showed that detection using the mMPN compared well to the ISO 6572:2002; sensitivity (92%), specificity (97%) and agreement (KAPPA 0·72). The quantitative comparison between the tMPN and mMPN methods showed that 92% of enumerations were less than ±1 log different (Student’s t‐test = 0·13). Financial analysis showed that the mMPN required 64% less media and 56% less labour than the tMPN. Conclusion: The mMPN is a consistent, easy to automate method for the enumeration of Salmonella from different poultry matrices. Significance and Impact of Study: The miniaturized MPN reduces the material and labour cost of the method and enables the uniform and accurate measurement of the effectiveness of intervention strategies in the control of Salmonella colonization of poultry.

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