Screening of food samples for carbamate and organophosphate pesticides using an electrochemical bioassay

Carlo, Michele Del; Mascini, Marcello; Pepe, Alessia; Diletti, Gianfranco; Compagnone, Darío

doi:10.1016/j.foodchem.2003.08.006

Cited by 78 publications

(40 citation statements)

References 21 publications

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“…Some organophosphate pesticides, such as Diazinon oxon (DZO, diethyl 2-isopropyl-6-methyl-4-pyrimidinyl phosphate), can also inhibit AChE activity via a similar mechanism. As one of the analytical methods, electrochemical measurements for AChE inhibition have been widely reported 26,27 . Our research group has also reported a colorimetric sensor for the detection of DZO based on the inhibition of AChE 28 .…”

Section: Detection Of Chemical Warfare Agentsmentioning

confidence: 99%

Field-deployable rapid multiple biosensing system for detection of chemical and biological warfare agents

et al. 2018

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The threat of biological and chemical terror acts remains a growing worldwide concern. There is therefore a need to develop appropriate technology for the detection of chemical and biological warfare agents, with early identification intended for use by first responders. Here, we disclose the developed autonomous air sampling and detection system for evaluation of the presence of chemical and biological warfare agents that can be harmful to the population. The current device utilizes the designed mist generator-assisted air collection system (338 l min − 1 ) and biosensing chip technologies, such as electrochemical measurement, Au nanoparticle-based localized surface plasmon resonance, and rapid microfluidic chip PCR for detection of minute concentrations lower than the mean lethal dose (LD 50 ) of nerve gases (sarin and VX), toxic proteins (BTX/A/Hc and ricin), and pathogens (anthrax simulant). An operation time of only 5-15 min is needed for the collection and detection; sample preparation is already integrated into the system without the need for direct human intervention. In addition to the system's sensitivity and ease of use, its portability makes it highly beneficial for first responders, which could aid in immediate risk assessment and mitigation of on-site events.

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Section: Detection Of Chemical Warfare Agentsmentioning

confidence: 99%

Field-deployable rapid multiple biosensing system for detection of chemical and biological warfare agents

et al. 2018

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“…Os resíduos de carbaril em amostras ambientais são geralmente determinados por técnicas cromatográficas [13][14][15][16][17] que incluem complicados pré-tratamentos ou clean-up das matrizes. Uma alternativa para essas análises são as técnicas eletroanalíticas empregando, por exemplo, eletrodos modificados [18][19][20][21][22] e DDB. 8,23 Esse material eletródico [24][25][26][27][28] apresenta ampla janela eletroquímica devida à pouca reatividade química da superfície do diamante, grande resistência a vários meios agressivos, baixas correntes capacitivas e robustez mecânica, que o caracteriza como um material ideal para experimentos sonoeletroquímicos.…”

Section: Introductionunclassified

Potencialidades do uso de ultrassom na determinação do pesticida carbaril empregando eletrodos de diamante

Garbellini¹,

Avaca²,

Salazar‐Banda³

2010

Quím. Nova

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Recebido em 16/12/09; aceito em 8/6/10; publicado na web em 18/10/10 ULTRASOUND POTENTIALITIES ON THE DETERMINATION OF THE PESTICIDE CARBARYL USING DIAMOND ELECTRODES. The potentiality of the use of ultrasound radiation in association with a boron-doped diamond electrode was evaluated on the voltammetric determination of the pesticide carbaryl. Improvements in the sensitivity, limit of detection and reproducibility of the measurements were observed due to both, the enhancement of mass transport and the cleaning of the electrode surface provided by ultrasound. Satisfactory recovery levels for carbaryl in pure water (96-98%) and pineapple juice (89-92%) for quiescent and sonovoltammetric methodologies were obtained. These methodologies can be alternative tools for the analyses of pesticides in fruit samples, mainly the insonated condition that improve the analytical performance and dispense intermediary cleanings of the electrode surface.Keywords: boron-doped diamond; sonoelectroanalysis; food samples. INTRODUÇÃOA determinação eletroanalítica direta de compostos orgânicos tóxicos, como os pesticidas, em amostras de alimentos é fortemente prejudicada pela adsorção e/ou insolubilização de produtos da oxidação destes compostos na superfície de eletrodos e, também, pela grande quantidade de interferentes presentes nessas amostras, diminuindo deste modo a sensibilidade e a reprodutibilidade das medidas eletroquímicas.Uma possibilidade de minimizar esse fenômeno de inativação eletródica é o uso da radiação de ultrassom em associação a estudos voltamétricos.1,2 A introdução do ultrassom em sistemas eletroquímicos é caracterizada pelo significativo aumento do transporte de massa que melhora a sensibilidade analítica em comparação a medidas quiescentes. O uso de ultrassom promove também a ativação da superfície eletródica por meio do processo de cavitação, que consiste na criação, ampliação e implosão de cavidades de vapor e gases.3 Esta melhora do transporte de massa é devida ao colapso de bolhas perto ou na interface eletrodo-solução, com a formação de fluxos de altas velocidades direcionados à superfície eletródica e à movimentação da bolha na região próxima ou dentro da camada de difusão do eletrodo. É interessante ressaltar que os fluxos acústicos são os maiores responsáveis pelo fluxo convectivo até a interface sólido-líquido. Diante disso, o aumento do transporte do material eletroativo à superfície eletródica pode ser medido assumindo uma diminuição da espessura da camada de difusão (δ). 4Esses efeitos sinérgicos do ultrassom foram demonstrados na determinação de nitrito em água pura 5 e de pesticidas e subprodutos (metilparation e 4-nitrofenol) em amostras de alimentos 6,7 sobre o eletrodo de diamante dopado com boro (DDB). Desta maneira, decidiu-se explorar a potencialidade da associação do ultrassom ao eletrodo de DDB para a determinação do pesticida carbaril (pertencente ao grupo dos carbamatos). Codognoto et al.8 mostraram a determinação de carbaril em águas naturais via oxidação do nitrogênio amida da molécul...

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“…Since there are large differences in cytotoxicity between different pesticide and herbicide preparations, there is also a need to develop simple means for pesticide screening (6) and for assessing damage caused by these pollutants to DNA, proteins and lipids (7,8). There is a mounting evidence for high acute toxicity of many pesticides and herbicides (9)(10)(11)(12)(13), including carcinogenesis (14)(15)(16), genotoxicity (1, 11,[17][18][19][20][21], and a number of different diseases.…”

Section: Introductionmentioning

confidence: 99%

Biosensors for the Detection of DNA Damage by Toxicants

Coopersmith¹,

Stobiecka²,

Hepel³

2010

ECS Trans.

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Novel DNA biosensors have been designed for testing DNA damage by toxicants. In this work, we describe the development of simple accelerated tests for DNA alterations by toxicants. To facilitate rapid testing, higher concentrations of reactants were investigated than those normally found in environmental and field samples. The sensors are based on electrochemical transduction with an intercalation redox probe. The damage and conformation changes of the immobilized DNA affect the penetration of the probe molecules and the redox current profiles. These robust sensors provide single base-pair mismatch sensitivity. In this work, the Methylene Blue and Nile Blue A redox probes were evaluated for testing DNA damage caused by an herbicide atrazine. Higher sensitivity was obtained using the Nile Blue (NB) dye as the active probe. Owing to the high sensitivity of the intercalator-probe uptake to the conformational alterations of DNA double-helix, the effects of DNA incubation in atrazine solutions could be assessed. We have found that the incubation of dsDNA with atrazine results in the increased activity of NB and an increased uptake of NB by the altered dsDNA. On the basis of this and other findings, the mechanism of atrazine interactions with DNA double-helix has been elucidated.

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Screening of food samples for carbamate and organophosphate pesticides using an electrochemical bioassay

Cited by 78 publications

References 21 publications

Field-deployable rapid multiple biosensing system for detection of chemical and biological warfare agents

Field-deployable rapid multiple biosensing system for detection of chemical and biological warfare agents

Potencialidades do uso de ultrassom na determinação do pesticida carbaril empregando eletrodos de diamante

Biosensors for the Detection of DNA Damage by Toxicants

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