Screening of fungal strains with potentiality to hydrolyze microalgal biomass by Fourier Transform Infrared Spectroscopy (FTIR)

Abstract

The use of fungi is a promising alternative for harnessing biomass after lipid extraction of microalgae since this biomass may contain relevant levels of carbohydrates that can be converted into other compounds of interest, such as sugars. Fourier Transform Infrared Spectroscopy (FTIR) has proved to be an efficient and environmentally less impacting tool for the selection of microorganisms with biotechnological potential. This study aimed to apply FTIR for the selection of fungal strains with potential to hydrolyze the biomass of the microalgae Desmodesmus subspicatus and Chlorella sp after lipid extraction. Eleven fungal strains were screened for residual biomass hydrolysis and FTIR was applied followed by multivariate analysis for the selection of filamentous fungi. The highest cell density was 28.7 × 10 6 cells mL-1 for Chlorella sp. and 15.8 × 10 6 cells mL-1 for D. subspicatus and the values of total carbohydrates content were 23.1 and 16.9%, respectively. Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were useful tools to screen fungal strains. After multivariate analysis, it was possible to observe that the fungi strains that presented the greatest ability to use microalgal biomass were Penicillium G12 due to the glucose and xylose sugars obtained after lipid extraction from D. subspicatus (with sugar yield of 9.4 and 6.6%, respectively) and Trichoderma auricularis for Chlorella sp. (with sugar yield of 12.9 and 9.6%, respectively). FTIR was successfully applied to screen fungal strains.

Economical alternatives for the production of fungal β-1,3-glucanase using easily obtainable industrial substrates

“…Resende et al (2002), using the same strain, demonstrated the production of xylanase and cellulase in sugarcane bagasse fermentation under solid-state conditions, with the average yield of xylanase being higher than that of cellulose. This feature is shared by other Trichoderma species (De Souza, et al, 2019). Thus, considering that L. cylindrica bushing is chemically composed of cellulose (65.2%), hemicellulose (17.5%), and lignin (15.2%) (Siqueira et al, 2010), it is possible to suggest that the biodegradability of the plant support occurred through the action of the xylanase produced by the fungus.…”