Screening of in vitro cytotoxicity by the adhesive film test

Srivastava, S.; Gorham, S. D.; Courtney, J. M.

doi:10.1016/0142-9612(90)90129-e

Cited by 15 publications

(8 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differences in cell proliferation were also found by Srivastava et al [32,33], who found decreased proliferation of L929 cells on collagen films compared with on plastic culture dishes. These studies also show differences in growth with different collagen-GAG composites.…”

Section: Discussionsupporting

confidence: 58%

“…succinylation and acetylation of the collagen, will influence cell attachment 0957-4530 ~C~) 1993 Chapman & Hall and growth [31]. Previous studies [32,33] by Srivastava et al showed that the incorporation of GAGs into collagen films could influence the growth and proliferation of L929 mouse fibroblasts. In this study we showed that a shape-change assay provides a quick and simple method of assessing cell behaviour on collagenous substrata.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The attachment, spreading and growth of baby hamster kidney cells on collagen, chemically modified collagen and collagen-composite substrata

Reid

Gorham

Lackie

1993

J Mater Sci: Mater Med

View full text Add to dashboard Cite

Various replicates of collagen substrata were prepared to study the attachment, growth and spreading of baby hamster kidney (BHK) cells. Cell attachment was measured in both the presence and absence of serum. Spreading and growth did not occur in the absence of serum. Attachment to fibrous collagen was less than that found with glass, rat-tail tendon collagen or films prepared from pepsin-solubilized collagen (PS-collagen). Incorporation of hyaluronate, heparin and protamine sulphate into the fibrous collagen and the acetylation of fibrous collagen had little effect. However, incorporation of chondroitin sulphate or chemical modification of fibrous collagen by either methylation or succinylation increased BHK cell attachment. In the absence of serum, the attachment to collagen, acetylated collagen and collagen composites was reduced. The reduction in attachment was marked with fibrous collagen and gelatin films, but less so with collagen composites, acetylated collagen, rat-tail tendon and PS-collagen films. Interestingly, attachment to succinylated collagen and methylated collagen was largely unaffected by the absence of serum, and possible reasons for this are discussed. Cell shape measurements showed decreased spreading of BHK cells on chemically modified collagen films, especially on gelatin films and dried PS-collagen gels. Cell shape and spreading on PS-collagen, rat-tail tendon collagen and collagen-composite films was found to be similar to that on fibrous collagen. BHK cell growth on fibrous collagen, chemically modified collagens, collagen composites, rat-tail tendon and PS-collagen films was similar to that found on plastic tissue culture substrata. Denaturation of fibrous collagen resulted in decreased growth, and BHK cell growth was markedly reduced on PS-collagen gels and dried gels.

show abstract

Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

The attachment, spreading and growth of baby hamster kidney cells on collagen, chemically modified collagen and collagen-composite substrata

Reid

Gorham

Lackie

1993

J Mater Sci: Mater Med

View full text Add to dashboard Cite

show abstract

“…When using liquid culture test systems there are two possibilities. Cells~may be cultured in the presence of extracts of biomaterial [1,2], or a biomaterial is put into the medium in (in-)direct contact with the cells [3][4][5][6][7][8]. When the latter system is used in long term testing, a disadvantage is the necessary changing of the medium, with removal of possible released cytotoxic products as a consequence.…”

Section: Introductionmentioning

confidence: 99%

Methylcellulose cell culture as a new cytotoxicity test system for biomaterials

Luyn

Wachem

Nieuwenhuis

et al. 1991

J Mater Sci: Mater Med

View full text Add to dashboard Cite

“…The method of cell culture in vitro is a quick and effective toxicity screening program [6]. It is a quick and precise method to evaluate the effect of different biomaterials on cytoactive by detecting Speriod cells with flow cytometry after the cell cultured with materials ET].…”

Section: Cytotoxieity Experimentsmentioning

confidence: 99%

Biocompatibility of MIM 316L stainless steel

Zhu

Wang

Zhao

et al. 2005

J Cent. South Univ. Technol.

View full text Add to dashboard Cite

To evaluate the biocompatibility of MIM 316L stainless steel, the percentage of S-period cells were detected by flow cytometry after L929 incubated with extraction of MIM 316L stainless steel, using titanium implant materials of clinical application as the contrast. Both materials were implanted in animal and the histopathologieal evaluations were carried out. The statistical analyses show that there are no significant differences between two groups (P>0.05), which demonstrates that MIM 316L stainless steel has a good biocompatibility.

show abstract

Screening of in vitro cytotoxicity by the adhesive film test

Cited by 15 publications

References 4 publications

The attachment, spreading and growth of baby hamster kidney cells on collagen, chemically modified collagen and collagen-composite substrata

The attachment, spreading and growth of baby hamster kidney cells on collagen, chemically modified collagen and collagen-composite substrata

Methylcellulose cell culture as a new cytotoxicity test system for biomaterials

Biocompatibility of MIM 316L stainless steel

Contact Info

Product

Resources

About