Screening of in vitro derived mutants of banana against nematodes

Abstract

Investigations were carried out to screen the in vitro derived mutants of banana cv. Robusta (Caveidish-AAA) and Rasthali (Silk-AAB) by using certain bio-chemical parameters including some enzyme activities. The mutants tested were Ro Im V 4 6-1-1, Ro Im V 4 6-1-2, Ro Im V 4 6-2-1, Si Im V 4 10-5-3, Si Im V 4 6-2-5 along with respective susceptible checks (Robusta and Rasthali), tolerant check (Anaikomban-AA) and resistant check (Pisang Lilin-AA). Various biochemical assays used were total phenol, tannin content, lignin content, peroxidase, polyphenol oxidase, phenyl alanine ammonia lyase and ascorbic acid oxidase. The results revealed that the mutants namely Ro Im V 4 6-1-1 and Si Im V 4 10-5-3 were found to be resistant while the mutant Ro Im V 4 6-2-1 was moderately resistant. The rest of the mutants namely Ro Im V 4 6-1-2 and Si Im V 4 6-2-5 were found to be susceptible to nematodes. The resistant and moderately resistant mutants of banana could be further used in breeding programmes as well as being recognized as potential cultivars of commerce.

Phytoparasitic Nematodes of Musa spp. with Emphasis on Sources of Genetic Resistance: A Systematic Review

“…In addition, Rocha et al [26] assessed the number of functional roots. Ramesh Kumar et al [65] evaluated the root lesions caused by R. similis and P. coffeae using the index described by Sundararaju [87] and the root necrosis index described by Carlier et al [88]. The highest index (5) was recorded in susceptible cultivars, while the lowest index (1) was recorded in the resistant cultivar Pisang Lilin.…”

“…The mutants from both groups, Ro Im V4 6-1-1 and Si Im V4 10-5-3, had a relatively lower index (2). Based on the percentage of root necrosis, the Im V4 6-1-1 and Si Im V4 10-5-3 mutants were considered resistant, while the Ro Im V4-6-2-1 mutant was moderately resistant [65].…”