Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5

Wang, Sheng; Wan, Li‐Jun; Ren, Hongyan; Xie, Zhixun; Xie, Liji; Huang, Jiaoling; Deng, Xianwen; Xie, Zhihui; Luo, Sisi; Li, Meng; Zeng, Tingting; Zhang, Yanfang; Zhang, Minxiu

doi:10.3389/fmicb.2022.998505

Cited by 7 publications

(12 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Understanding the related functions of IFI27L1 is of guiding significance for the study of IFI6. A few literatures have shown that IFI27L1 is a protein that can promote apoptosis (Xie et al, 2022). This provides clues for future research on the function of avian IFI6.…”

Section: Discussionmentioning

confidence: 80%

“…Studies have found that ARV infection activates the expression of many innate immune response-related factors in a host. ARV S1133 infection in specific-pathogen-free chickens can induce changes in the expression of various innate immunity-related factors, including IFN-α, IFN-β, IFN-γ, IL-6, IL-17, IL-18, MX, IFITM3, PKR, OAS, IFIT5, ISG12, VIPERIN, and IFI6, in chicken peripheral blood lymphocytes and joints ( Xie et al, 2019 ; Wang et al, 2021 , 2022 ). In addition, many studies have aimed to understand the intracellular molecular mechanisms underlying the effects of ARV infection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chicken IFI6 inhibits avian reovirus replication and affects related innate immune signaling pathways

Wan,

Wang,

Xie

et al. 2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Interferon-alpha inducible protein 6 (IFI6) is an important interferon-stimulated gene. To date, research on IFI6 has mainly focused on human malignant tumors, virus-related diseases and autoimmune diseases. Previous studies have shown that IFI6 plays an important role in antiviral, antiapoptotic and tumor-promoting cellular functions, but few studies have focused on the structure or function of avian IFI6. Avian reovirus (ARV) is an important virus that can exert immunosuppressive effects on poultry. Preliminary studies have shown that IFI6 expression is upregulated in various tissues and organs of specific-pathogen-free chickens infected with ARV, suggesting that IFI6 plays an important role in ARV infection. To analyze the function of avian IFI6, particularly in ARV infection, the chicken IFI6 gene was cloned, a bioinformatics analysis was conducted, and the roles of IFI6 in ARV replication and the innate immune response were investigated after the overexpression or knockdown of IFI6 in vitro. The results indicated that the molecular weight of the chicken IFI6 protein was approximately 11 kDa and that its structure was similar to that of the human IFI27L1 protein. A phylogenetic tree analysis of the IFI6 amino acid sequence revealed that the evolution of mammals and birds was clearly divided into two branches. The evolutionary history and homology of chickens are similar to those of other birds. Avian IFI6 localized to the cytoplasm and was abundantly expressed in the chicken lung, intestine, pancreas, liver, spleen, glandular stomach, thymus, bursa of Fabricius and trachea. Further studies demonstrated that IFI6 overexpression in DF-1 cells inhibited ARV replication and that the inhibition of IFI6 expression promoted ARV replication. After ARV infection, IFI6 modulated the expression of various innate immunity-related factors. Notably, the expression patterns of MAVS and IFI6 were similar, and the expression patterns of IRF1 and IFN-β were opposite to those of IFI6. The results of this study further advance the research on avian IFI6 and provide a theoretical basis for further research on the role of IFI6 in avian virus infection and innate immunity.

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Chicken IFI6 inhibits avian reovirus replication and affects related innate immune signaling pathways

Wan,

Wang,

Xie

et al. 2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…IFIT5 inhibits the replication of many viruses in the host through the type I IFN signaling pathway. For example, it plays a key role in inhibiting the replication of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) that infects porcine pulmonary microvascular endothelial cells [38,39]. HAX-1 is an apoptosis inhibitor that is primarily located in the mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Analysis of lncRNA and Target Genes Induced by Influenza A Virus Infection in MDCK Cells

Liu,

Pei,

Wang

et al. 2023

Vaccines

View full text Add to dashboard Cite

Background: The MDCK cell line is the primary cell line used for influenza vaccine production. Using genetic engineering technology to change the expression and activity of genes that regulate virus proliferation to obtain high-yield vaccine cell lines has attracted increasing attention. A comprehensive understanding of the key genes, targets, and molecular mechanisms of viral regulation in cells is critical to achieving this goal, yet the post-transcriptional regulation mechanism involved in virus proliferation—particularly the effect of lncRNA on influenza virus proliferation—is still poorly understood. Therefore, this study used high-throughput RNA-seq technology to identify H1N1 infection-induced lncRNA and mRNA expression changes in MDCK cells and explore the regulatory relationship between these crucial lncRNAs and their target genes. Results: In response to H1N1 infection in MDCK cells 16 h post-infection (hpi) relative to uninfected controls, we used multiple gene function annotation databases and initially identified 31,501 significantly differentially expressed (DE) genes and 39,920 DE lncRNAs (|log2FC| > 1, p < 0.05). Among these, 102 lncRNAs and 577 mRNAs exhibited predicted correlations with viral response mechanisms. Based on the magnitude of significant expression differences, related research, and RT-qPCR expression validation at the transcriptional level, we further focused on 18 DE mRNAs and 32 DE lncRNAs. Among these, the differential expression of the genes RSAD2, CLDN1, HCLS1, and IFIT5 in response to influenza virus infection was further verified at the protein level using Western blot technology, which showed results consistent with the RNA-seq and RT-qPCR findings. We then developed a potential molecular regulatory network between these four genes and their six predicted lncRNAs. Conclusions: The results of this study will contribute to a more comprehensive understanding of the molecular mechanism of host cell non-coding RNA-mediated regulation of influenza virus replication. These results may also identify methods for screening target genes in the development of genetically engineered cell lines capable of high-yield artificial vaccine production.

show abstract

“…The changes in the expression of ARV σC gene and innate immune signaling pathway-correlated molecules were detected by real-time fluorescence quantitative PCR (RT–qPCR). The utilized primers [ 12 , 14 , 28 ] were described previously ( Table 1 ). In addition, the abovementioned culture medium was diluted for the infection of DF-1 cells.…”

Section: Methodsmentioning

confidence: 99%

“…IFN induces the production of many interferon-stimulated genes (ISGs) by activating the JAK/STAT signaling pathway [ 10 ], and ISGs are the main executors of IFN antiviral functions [ 11 ]. Studies have shown that ARV infection can induce the transcriptional expression of IFN-α, IFN-β and ISGs such as IFITM1, IFITM3, IFIT5, Mx, ISG12 and other cytokines in various tissues and organs; at the early stage of ARV infection, IFITM3 is significantly upregulated in peripheral blood lymphocytes, joints, the thymus and the bursa of Fabricius and shows a consistent trend with the viral load of ARV in these tissues and organs, which suggests that IFITM3 plays a crucial role in ARV infection [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Chicken IFITM3 Gene Expression and Its Effect on Avian Reovirus Replication

Ren,

Wang,

Xie

et al. 2024

Viruses

Self Cite

View full text Add to dashboard Cite

Interferon-inducible transmembrane protein 3 (IFITM3) is an antiviral factor that plays an important role in the host innate immune response against viruses. Previous studies have shown that IFITM3 is upregulated in various tissues and organs after avian reovirus (ARV) infection, which suggests that IFITM3 may be involved in the antiviral response after ARV infection. In this study, the chicken IFITM3 gene was cloned and analyzed bioinformatically. Then, the role of chicken IFITM3 in ARV infection was further explored. The results showed that the molecular weight of the chicken IFITM3 protein was approximately 13 kDa. This protein was found to be localized mainly in the cytoplasm, and its protein structure contained the CD225 domain. The homology analysis and phylogenetic tree analysis showed that the IFITM3 genes of different species exhibited great variation during genetic evolution, and chicken IFITM3 shared the highest homology with that of Anas platyrhynchos and displayed relatively low homology with those of birds such as Anser cygnoides and Serinus canaria. An analysis of the distribution of chicken IFITM3 in tissues and organs revealed that the IFITM3 gene was expressed at its highest level in the intestine and in large quantities in immune organs, such as the bursa of Fabricius, thymus and spleen. Further studies showed that the overexpression of IFITM3 in chicken embryo fibroblasts (DF-1) could inhibit the replication of ARV, whereas the inhibition of IFITM3 expression in DF-1 cells promoted ARV replication. In addition, chicken IFITM3 may exert negative feedback regulatory effects on the expression of TBK1, IFN-γ and IRF1 during ARV infection, and it is speculated that IFITM3 may participate in the innate immune response after ARV infection by negatively regulating the expression of TBK1, IFN-γ and IRF1. The results of this study further enrich the understanding of the role and function of chicken IFITM3 in ARV infection and provide a theoretical basis for an in-depth understanding of the antiviral mechanism of host resistance to ARV infection.

show abstract

Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5

Abstract: Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5.

Cited by 7 publications

References 34 publications

Chicken IFI6 inhibits avian reovirus replication and affects related innate immune signaling pathways

Chicken IFI6 inhibits avian reovirus replication and affects related innate immune signaling pathways

Transcriptional Analysis of lncRNA and Target Genes Induced by Influenza A Virus Infection in MDCK Cells

Analysis of Chicken IFITM3 Gene Expression and Its Effect on Avian Reovirus Replication

Contact Info

Product

Resources

About