Screening of key miRNAs related with the differentiation of subcutaneous adipocytes and the validation of miR-133a-3p functional significance in goats

Abstract

Screening of key miRNAs related with the differentiation of subcutaneous adipocytes and the validation of miR-133a-3p functional significance in goats ABSTRACTObjective: Adipocyte differentiation is regulated by a variety of functional genes and non-coding RNAs. However, the role of miRNAs in lipid deposition of goat white adipose tissue is still unclear.Therefore, this study revealed the miRNA expression profile in goat subcutaneous adipocytes by sRNA-seq. Methods:The miRNA expressed in goat subcutaneous preadipocytes and the mature adipocytes were sequenced by sRNA-seq. The differentially expressed miRNAs (DEm) were screened, and GO and KEGG analyses were performed. Gain-of-function and loss-of-function combined with Oil Red O staining, Bodipy staining and qPCR were utilized to determine the effect of miR-133a-3p on adipocyte differentiation.Results: A total of 218 DEm were screened out. The target genes of these DEm were significantly enriched in GO items such as biological regulation and in KEGG terms such as FAK signaling pathway and MAPK signaling pathway. qPCR verified that the expression trend of miRNA was consistent with miRNA-seq. The gain-of-function or loss-of-function of miR-133a-3p showed that it promoted or inhibited the accumulation of lipid droplets, and C/EBPα and C/EBPβ were extremely significantly up-regulated or down-regulated respectively (P<0.01), the loss-of-function also led to a significant down-regulation of PPARγ (P<0.01). Conclusion:This study successfully identified miRNAs expression patterns in goat subcutaneous adipocytes, and functional identification indicates that miR-133a-3p is a positive regulator of the differentiation process of goat subcutaneous adipocytes. Our results lay the foundation for the molecular mechanism of lipid deposition in meat-source goats from the perspective of miRNA.

Impact of protein source from legume forages on lipid regulation and growth on native ram lambs reared extensively in the south coast of West Java, Indonesia

“…This study reveals that legume protein can influence lipid metabolism in ram lambs cells. Previous researchers (Li et al 2022;Zsolnai et al 2022) in pigs have shown similar results to the current study; it has been reported that feeding high protein diets can reduce lipogenesis activity.…”

“…Studies reported by Adriani and Mushawwir (2020), Mushawwir et al (2020), andSun et al (2022) show that increasing insulin will increase the absorption of glucose into the cytoplasm. An increase in glucose in the cytoplasm stimulates an increase in acetyl-CoA so that the activity of glucose-6-pyruvat dehydrogenase (G-6-PDH) decreases (Mushawwir et al 2020;Li et al 2022). Furthermore, a decrease in G-6-PDH causes a decrease in NADPH production (Sejian et al 2021;Rahmania et al 2022).…”

Guanidinoacetic Acid Attenuates Adipogenesis through Regulation of miR-133a in Sheep

“…Considering that SIRT1 suppresses adipogenesis through inhibition of PPARγ activity [37] and activation of Wnt/βcatenin signaling in white adipose tissue [38], these data suggested that miR-133a positively regulated adipogenesis through modulation of Sirt1 in sheep SVF cells. Similarly, a recent report from Li et al suggests that miR-133a serves as a positive regulator of adipogenesis in goat subcutaneous preadipocytes [39]. To further elucidate whether GAA suppresses adipogenesis through regulation of miR-133a, sheep SVF cells treated with GAA were transfected with miR-133a mimics.…”