Screening of Lactobacillus strains for their ability to bind Benzo(a)pyrene and the mechanism of the process

Zhao, Huanxin; Fang, Zhou; Qi, Yeqiong; Dziugan, Piotr; Feng-ling, Bai; Walczak, Piotr; Zhang, Bolin

doi:10.1016/j.fct.2013.05.040

Cited by 63 publications

(47 citation statements)

References 17 publications

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“…() and Zhao et al . () found that LABs and probiotics can bind BaP and show antimutagenic activities against BaP. It has been reported by Zhu et al .…”

Section: Discussionmentioning

confidence: 70%

“…Furthermore, probiotics can adsorb different toxic compounds in their cell surface through physical interactions (Zhao et al . ; Serrano‐Niño et al . ).…”

Section: Introductionmentioning

confidence: 99%

“…Almost all of the studies which evaluated the ability of LABs and probiotics in removing toxins, indicated that binding of toxin to cell wall is the most possible mechanism and therefore, this phenomenon can be affected by various factors such as pH, type and concentration of toxin, strain type and its population, time and media (Zhao et al . ; Serrano‐Niño et al . ; Khorshidian et al .…”

Section: Introductionmentioning

confidence: 99%

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In vitroremoval of polycyclic aromatic hydrocarbons by lactic acid bacteria

et al. 2019

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Aims The current study aimed to investigate the ability of lactic acid bacteria (LABs) in removing four polycyclic aromatic hydrocarbons (PAHs) namely, benzo(a)pyrene (BaP), benz(a)anthracene (BaA), chrysene (Chr) and benzo(b)fluoranthene (BbF) from contaminated phosphate buffer saline (PBS). Method and Results The effect of initial PAH concentrations (5, 10, 15, 20 μg ml−1), bacterial population (107, 108, 109, 1010 CFU per ml) and pH (3, 5, 7) was studied to evaluate bacterial binding ability. All the tested bacteria could remove BaA, Chr, BbF and BaP from phosphate buffer solution and in almost all assays, removing of PAHs was as follows: BaP>Chr>BaA>BaF. Bifidobacterium lactis BB‐12 had the lowest binding rate for all four PAHs, while the highest binding ability was related to Lactobacillus acidophilus LA‐5. Moreover, cell viability was not required for the binding ability and even acid‐treated, heat‐treated and ultrasonic‐treated bacterial cells showed more binding ability. The results showed that the bacteria–PAH complex was irreversible after washing with PBS. Conclusions The removal of PAHs was significantly related to pH of media, strains of bacteria, type and concentration of PAHs Significance and Impact of the Study This study has been focused on the reduction of polycyclic aromatic hydrocarbons using LABs and probiotics. Our results showed that not only live strains but also inactivated tested strains are able to remove PAHs from aqueous media, presenting new methods to diminish the amount of these contaminants in foods. Furthermore, the results of this study can be used in future research on evaluating the effects of oral administration of probiotic supplements and even dead probiotic strains on reducing PAHs in humans.

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“…() and Zhao et al . () found that LABs and probiotics can bind BaP and show antimutagenic activities against BaP. It has been reported by Zhu et al .…”

Section: Discussionmentioning

confidence: 70%

“…Furthermore, probiotics can adsorb different toxic compounds in their cell surface through physical interactions (Zhao et al . ; Serrano‐Niño et al . ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitroremoval of polycyclic aromatic hydrocarbons by lactic acid bacteria

et al. 2019

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“…Many interesting mutants with high activity were identified from the metagenome of bacteria in chemical plant sludge. The ability of the CueO protein and its mutants to degrade benzo[α]pyrene, a mutagenic and highly carcinogenic environmental polycyclic aromatic hydrocarbon (PAH), was evaluated (Zhao et al 2013). The results indicate that the cloned cueO mutants could catalyze transformation of PAHs, similar to the function of fungal laccase from Trametes versicolor (Zeng et al 2011(Zeng et al , 2016Hullo et al 2001;Roberts et al 2002).…”

Section: Open Accessmentioning

confidence: 99%

Identification of bacterial laccase cueO mutation from the metagenome of chemical plant sludge

Yue

Yang

Zhao

et al. 2017

Bioresour. Bioprocess.

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Background:The metagenome contains plenty of genetic resources and can be used to search for the novel gene or mutant. Results:In this study, the bacterial laccase gene (cueO) with single or multiple mutations was directly cloned based on the metagenome of a chemical plant sludge. An interesting mutation (G276R) was identified from those cloned mutants. The other mutants (G276N, G276Y, and G276K) with improved catalytic efficiency were identified by the saturation mutagenesis on residue G276. The optimal temperature for wild-type CueO enzyme activity was about 70 °C, compared to 60 °C, 50 °C, 50 °C, and 30 °C for the G276R, G276N, G276Y, and G276K mutant enzymes, respectively. The catalytic efficiency (k cat /K m ) with 8 mmol Cu 2+ of the G276R, G276N, G276Y, and G276K mutants was 1.2-, 2.7-, 1.3-, and 2.7-fold, respectively, compared to the wild-type enzyme. In addition, the mutants G276R, G276N, G276Y, and G276K oxidized the carcinogen benzo[α]pyrene more efficiently compared to the wild-type enzyme. Conclusion:All of the results indicate that G276 of CueO plays an important role in enzyme activity, and the useful mutants can be identified based on the metagenome.

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“…Furthermore, other mechanisms could explain the high interest in the use of probiotics as a potential preventive food against CRC, such as competitive exclusion of pathogenic intestinal flora (Rafter, 2003), alteration of intestinal flora enzyme activity (Goldin & Gorbach, 1984), reduction of carcinogenic secondary bile acids (De Boever et al, 2000), binding of carcinogens (Fotiadis, Stoidis, Spyropoulos, & Zografos, 2008), production of anti-tumorigenic or antimutagenic compounds (Zhao et al, 2013). Sivieri et al (2008) demonstrated that the oral administration of Enterococcus faecium CRL 183 resulted in a significant reduction of DMH-induced ACF formation in male Wistar rats.…”

Section: Introductionmentioning

confidence: 99%