Screening of ligninolytic fungi for biological pretreatment of lignocellulosic biomass

Abstract

https://mc06.manuscriptcentral.com/cjm-pubs In order to identify white rot fungi with high potential in biological pretreatment of lignocellulosic biomass, 18 preliminary screening was carried out on plates by testing strains' abilities to oxidize guaiacol and decolorize the 19 dyes, azure B and Poly R-478. Of the eighty six strains screened, sixteen were further used for secondary screening 20 based on their ligninolytic ability. However, low manganese peroxidase activity and no lignin peroxidase activity 21 were detected. Strain BBEL0970 was proved to be the most efficient in laccase production, which was finally 22 identified as T. versicolor by the analysis of ribosomal DNA internal transcribed spacer gene sequence. Combining 23 laccase production with biological pretreatment, the replacement of glucose with barley straw significantly improved 24 the laccase activity up to 10.3 U/ml, which provided evidence toward potential utilization of barley straw in laccase 25 production by BBEL0970. Simultaneously, comparison in thermogravimetry analysis of the untreated and pretreated 26 barley straw in liquid fermentation of laccase also demonstrated the high potential of BBEL0970 in biological 27 pretreatment of lignocellulosic biomass. This work sheds light on further exploration on integrated process of low-28 cost laccase production and efficient biological pretreatment of barley straw by T. versicolor BBEL0970. 29

Fungal pretreatment of agricultural residues for bioethanol production

“…It can be observed that from eight lignocellulosic biomasses, corn stover presented the highest lignin delignification and lowest total weight loss which is reflected in a higher lignin selectivity removal; while it obtained the second highest results of final holocellulosic digestibility, after wheat straw. In a previous study, in which 33 strains of WRF were evaluated on three lignocellulosic biomasses (bamboo, rice straw and corn stover) it was found that Irpex lacteus was the fungus that produced the highest saccharification rates after 15 d of fungal pretreatment (Xu et al, 2009).…”

Screening and Production of Manganese Peroxidase from Fusarium sp. on Residue Materials

“…MnP producing strains were detected by inoculating fungal mycelia on basal salts medium containing 0.01% guaiacol as described previously [11]. After 24 days culture, the MnP producing strains were confirmed by a halo to colony ratio of oxidized guacicol around the fungal colonies.…”

Cellulomonas Triticagri Sp. Nov., A Novel Actinomycete Isolated From The Rhizosphere Soil Of Wheat (Triticum Aestivum L.)

“…The utilization of sole carbon and nitrogen sources, decomposition of cellulose, hydrolysis of starch and aesculin, reduction of nitrate, coagulation of milk, liquefaction of gelatin and production of H 2 S were examined as described previously (Gordon et al 1974;Yokota et al 1993). The ability of lignin degradation was determined by the azure B plate decolorization method (Xu et al 2015).…”