Screening of Lipase-Producing Bacteria and Optimization of Lipase-Mediated Biodiesel Production from Jatropha curcas Seed Oil Using Whole Cell Approach

Haq, Abdul; Adeel, Sana; Khan, Alam Zeb; Rana, Qurrat ul ain; Khan, Mahnoor; Rafiq, Muhammad; Ishfaq, Muhammad; Khan, Samiullah; Shah, Aamer Ali; Hasan, Fariha; Ahmed, Safia; Badshah, Malik

doi:10.1007/s12155-020-10156-1

Cited by 16 publications

(5 citation statements)

References 49 publications

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“…For the biological trans-esterification of oil, lipase-producing bacteria were used through a whole-cell approach. Bacterial strain Q4 was isolated from vegetable oil industry wastewater and screened for lipase production in a previous study and was utilized for this purpose (Rana 2019 ; Haq et al 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Utilization of microalgae for agricultural runoff remediation and sustainable biofuel production through an integrated biorefinery approach

Rana,

Latif,

Perveen

et al. 2024

Bioresour. Bioprocess.

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Generally wastewater such agricultural runoff is considered a nuisance; however, it could be harnessed as a potential source of nutrients like nitrates and phosphates in integrated biorefinery context. In the current study, microalgae Chlorella sp. S5 was used for bioremediation of agricultural runoff and the leftover algal biomass was used as a potential source for production of biofuels in an integrated biorefinery context. The microalgae Chlorella sp. S5 was cultivated on Blue Green (BG 11) medium and a comprehensive optimization of different parameters including phosphates, nitrates, and pH was carried out to acquire maximum algal biomass enriched with high lipids content. Dry biomass was quantified using the solvent extraction technique, while the identification of nitrates and phosphates in agricultural runoff was carried out using commercial kits. The algal extracted lipids (oils) were employed in enzymatic trans-esterification for biodiesel production using whole-cell biomass of Bacillus subtilis Q4 MZ841642. The resultant fatty acid methyl esters (FAMEs) were analyzed using Fourier transform infrared (FTIR) spectroscopy and gas chromatography coupled with mass spectrometry (GC–MS). Subsequently, both the intact algal biomass and its lipid-depleted algal biomass were used for biogas production within a batch anaerobic digestion setup. Interestingly, Chlorella sp. S5 demonstrated a substantial reduction of 95% in nitrate and 91% in phosphate from agricultural runoff. The biodiesel derived from algal biomass exhibited a noteworthy total FAME content of 98.2%, meeting the quality standards set by American Society for Testing and Materials (ASTM) and European union (EU) standards. Furthermore, the biomethane yields obtained from whole biomass and lipid-depleted biomass were 330.34 NmL/g VSadded and 364.34 NmL/g VSadded, respectively. In conclusion, the findings underscore the potent utility of Chlorella sp. S5 as a multi-faceted resource, proficiently employed in a sequential cascade for treating agricultural runoff, producing biodiesel, and generating biogas within the integrated biorefinery concept. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

Utilization of microalgae for agricultural runoff remediation and sustainable biofuel production through an integrated biorefinery approach

Rana,

Latif,

Perveen

et al. 2024

Bioresour. Bioprocess.

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“…The column temperature was set at 250 • C as the maximum temperature. The yield of fatty acid ethyl esters (FAEEs) was calculated using the following formula (Equation ( 5)) [60]:…”

Section: Fatty Acid Ethyl Ester Analysismentioning

confidence: 99%

Thermostable CaCO3-Immobilized Bacillus subtilis Lipase for Sustainable Biodiesel Production from Waste Cooking Oil

Alshehri,

Alghamdi,

Khalel

et al. 2024

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Due to the increasing demand for green processes in renewable energy production, the extracellular Bacillus subtilis B-1-4 lipase was used as a biocatalyst for producing biodiesel from waste cooking oil. Response surface methodology was employed for the optimization of enzyme production. Lipase activity was modeled with a quadratic function of four factors that primarily influence the culture medium. Thanks to this model, an optimal lipase activity of 1.7 ± 0.082 U/mL was achieved with the best culture medium composition: 16 g/L of tryptone, 15 g/L of yeast extract, 15 g/L of NaCl, and a 0.15 initial optical density at 600 nm (OD600 nm). The maximal lipase activity was measured at 45 °C and pH 8, using para-nitrophenyl palmitate as a substrate. The enzyme maintained above 94% and 99% of its initial activity at temperatures ranging from 40 to 50 °C and at pH 8, respectively. Moreover, it exhibited a higher residual activity than other Bacillus lipases in the presence of organic solvents. Residual activities of 86.7% and 90.2% were measured in the presence of isopropanol and ethanol, respectively. The lipase was immobilized by adsorption onto CaCO3 powder. FT-IR and SEM were used to characterize the surface-modified support. After immobilization, a lipase activity of 7.1 U/mg of CaCO3 was obtained. Under the optimized conditions, the highest biodiesel yield of 71% was obtained through the transesterification of waste cooking oil using the CaCO3-immobilized Bacillus subtilis lipase. This research reveals a method for the utilization of waste cooking oil for biodiesel production using an efficient immobilized thermostable lipase, providing environmental and economic security.

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“…Qualitative screening was performed to evaluate the lipaseproducing abilities of the strains in peptone agar medium following the published method (Haq et al, 2020) with some modifications: the assay was conducted at pH 6 and supplemented with 1% separately sterilized Tween 20. Ten μL of either single or combined strains were pipetted onto peptone agar medium and incubated at 28°C for 120 h. A clear zone formed around a colony was used as an indicator of the lipase activity of the bacterial strains.…”

Section: Lipase Activitymentioning

confidence: 99%

Antagonistic and plant growth promotion of rhizobacteria against Phytophthora colocasiae in taro

et al. 2022

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Taro leaf blight caused by Phytophthora colocasiae adversely affects the growth and yield of taro. The management of this disease depends heavily on synthetic fungicides. These compounds, however, pose potential hazards to human health and the environment. The present study aimed to investigate an alternative approach for plant growth promotion and disease control by evaluating seven different bacterial strains (viz., Serratia plymuthica, S412; S. plymuthica, S414; S. plymuthica, AS13; S. proteamaculans, S4; S. rubidaea, EV23; S. rubidaea, AV10; Pseudomonas fluorescens, SLU-99) and their different combinations as consortia against P. colocasiae. Antagonistic tests were performed in in vitro plate assays and the effective strains were selected for detached leaf assays and greenhouse trials. Plant growth-promoting and disease prevention traits of selected bacterial strains were also investigated in vitro. Our results indicated that some of these strains used singly (AV10, AS13, S4, and S414) and in combinations (S4+S414, AS13+AV10) reduced the growth of P. colocasiae (30−50%) in vitro and showed disease reduction ability when used singly or in combinations as consortia in greenhouse trials (88.75−99.37%). The disease-suppressing ability of these strains may be related to the production of enzymes such as chitinase, protease, cellulase, and amylase. Furthermore, all strains tested possessed plant growth-promoting traits such as indole-3-acetic acid production, siderophore formation, and phosphate solubilization. Overall, the present study revealed that bacterial strains significantly suppressed P. colocasiae disease development using in vitro, detached leaf, and greenhouse assays. Therefore, these bacterial strains can be used as an alternative strategy to minimize the use of synthetic fungicides and fertilizers to control taro blight and improve sustainable taro production.

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Screening of Lipase-Producing Bacteria and Optimization of Lipase-Mediated Biodiesel Production from Jatropha curcas Seed Oil Using Whole Cell Approach

Cited by 16 publications

References 49 publications

Utilization of microalgae for agricultural runoff remediation and sustainable biofuel production through an integrated biorefinery approach

Utilization of microalgae for agricultural runoff remediation and sustainable biofuel production through an integrated biorefinery approach

Thermostable CaCO3-Immobilized Bacillus subtilis Lipase for Sustainable Biodiesel Production from Waste Cooking Oil

Antagonistic and plant growth promotion of rhizobacteria against Phytophthora colocasiae in taro

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