Screening of marine bacteria for fucoidanases

Bakunina, I. Yu.; Shevchenko, L. S.; Nedashkovskaya, Olga I.; Шевченко, Н. М.; Alekseeva, S. A.; Mikhailov, Valery V.; Zvyagintseva, T. N.

doi:10.1007/bf02756738

Cited by 28 publications

(21 citation statements)

References 14 publications

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“…Until now, enzymes that degrade fucoidans were found in the following marine organisms: bacteria [25,26,27], invertebrates [28,29,30,31,32] and some fungi [33]. However, the fucoidanases produced by these organisms have low activities.…”

Section: Introductionmentioning

confidence: 99%

Hydrolysis of Fucoidan by Fucoidanase Isolated from the Marine Bacterium, Formosa algae

et al. 2013

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Intracellular fucoidanase was isolated from the marine bacterium, Formosa algae strain KMM 3553. The first appearance of fucoidan enzymatic hydrolysis products in a cell-free extract was detected after 4 h of bacterial growth, and maximal fucoidanase activity was observed after 12 h of growth. The fucoidanase displayed maximal activity in a wide range of pH values, from 6.5 to 9.1. The presence of Mg2+, Ca2+ and Ba2+ cations strongly activated the enzyme; however, Cu2+ and Zn2+ cations had inhibitory effects on the enzymatic activity. The enzymatic activity of fucoidanase was considerably reduced after prolonged (about 60 min) incubation of the enzyme solution at 45 °C. The fucoidanase catalyzed the hydrolysis of fucoidans from Fucus evanescens and Fucus vesiculosus, but not from Saccharina cichorioides. The fucoidanase also did not hydrolyze carrageenan. Desulfated fucoidan from F. evanescens was hydrolysed very weakly in contrast to deacetylated fucoidan, which was hydrolysed more actively compared to the native fucoidan from F. evanescens. Analysis of the structure of the enzymatic products showed that the marine bacteria, F. algae, synthesized an α-l-fucanase with an endo-type action that is specific for 1→4-bonds in a polysaccharide molecule built up of alternating three- and four-linked α-l-fucopyranose residues sulfated mainly at position 2.

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Section: Introductionmentioning

confidence: 99%

Hydrolysis of Fucoidan by Fucoidanase Isolated from the Marine Bacterium, Formosa algae

et al. 2013

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“…Flavobaterium (Avendano-Herrera et al, 2005;Urvantseva et al, 2006) and Cytophaga (Bakunina et al, 2000) were also reported to be isolated from the Apostichopus japonicus. Flavobacterium species, including Flavobacterium branchiophilum (Lumsden et al, 1995) and Flavobacterium psychrophilum (Crump et al, 2001), are well-known Gramnegative pathogenic yellow-pigmented bacteria that have been associated with diseased fish.…”

Section: Discussionmentioning

confidence: 96%

“…Pseudoalteromonas citrea KMM 3297 (Bakunina et al, 2002) and Alteromonas sp. (Bakunina et al, 2000), as well as Shewanella colwelliana (Ivanova et al, 2003), the closest strain to clone 4, were reported to have been isolated from the holothurian Apostichopus japonicus. Additionally, Pseudoalteromonas elyakovii was reported to have been isolated from spot-wounded fronds of Laminaria japonica (Sawabe et al, 2000), indicating that it is a probable pathogenic agent.…”

Section: Discussionmentioning

confidence: 98%

16S rRNA gene phylogenesis of culturable predominant bacteria from diseased Apostichopus japonicus (Holothuroidea, Echinodermata)

Jia

et al. 2009

J. Ocean Univ. China

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Cultured Apostichopus japonicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease, pathogens of which are supposed to be bacteria by most researchers, is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao, China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies, and touchdown PCR was performed to amplify the target sequences. The results suggest that γ-proteobacteria (Alteromonadales and Vibrionales) of CFB group, many strains of which have been also determined as pathogens in other marine species, are the predominant bacterial genera of the diseased Apostichopus japonicus individuals.

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“…Fucoidan‐utilizing bacterial strains are appropriate starting points for preparing fucoidan‐degrading enzymes (Furukawa et al. 1992; Bakunina et al. 2000; Sakai et al.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the enzyme could assist in elucidating the definite structure of fucoidan (Sakai et al 2003b) and mechanisms of its bioactivities. Fucoidan-utilizing bacterial strains are appropriate starting points for preparing fucoidan-degrading enzymes (Furukawa et al 1992;Bakunina et al 2000;Sakai et al 2003cSakai et al , 2004Descamps et al 2006). There have been an increasing number of reports on the degrading enzymatic activities of microorganisms towards various algal fucoidans over last two decades, and some algal fucoidan-degrading enzymes have been well characterized and furthermore been gene cloned and expressed, such as the fucanase FcnA of Mariniflexile fucanivorans Descamps et al 2006;Barbeyron et al 2008) and the two sulfated fucan hydrolases, Fda1 and Fda2, of Altermonas sp.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a sea cucumber fucoidan-utilizing marine bacterium

Chang

Xue

Tang

et al. 2010

Letters in Applied Microbiology

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Aims: To isolate a fucoidan‐utilizing strain from seawater for sea cucumber fucoidan degradation. Methods and Results: The utilization of sea cucumber fucoidan was monitored by H2SO4–phenol assay for neutral sugar. The bacterium CZ1127 was isolated from seawater and shown to have a relatively large maximum fucoidan‐utilizing rate of 81·5%. CZ1127 was confirmed to belong to the family Flavobacteriaceae by 16S rDNA and physiological analyses. This strain has an ability to utilize fucoidans extracted from various sea cucumbers to different degrees. Both extracellular and intracellular enzymes of CZ1127 could degrade sea cucumber fucoidan, as confirmed by high‐performance size exclusion chromatography. The Mr of sea cucumber fucoidan could be reduced from 792·6 kDa to at least 3·7 kDa by the crude intracellular enzyme of this strain. Conclusions: The marine bacterial strain CZ1127, which belongs to the family Flavobacteriaceae, was found to utilize various sea cucumber fucoidans and furthermore showed promise in sea cucumber fucoidan enzymatic degradation and oligosaccharide preparation. Significance and Impact of the Study: The finding of a novel source can be applied in sea cucumber fucoidan enzymatic degradation. Furthermore, it is the first definite report of a bacterial strain that can utilize the fucoidans from various sea cucumbers.

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Screening of marine bacteria for fucoidanases

Cited by 28 publications

References 14 publications

Hydrolysis of Fucoidan by Fucoidanase Isolated from the Marine Bacterium, Formosa algae

Hydrolysis of Fucoidan by Fucoidanase Isolated from the Marine Bacterium, Formosa algae

16S rRNA gene phylogenesis of culturable predominant bacteria from diseased Apostichopus japonicus (Holothuroidea, Echinodermata)

Isolation and characterization of a sea cucumber fucoidan-utilizing marine bacterium

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