Screening of medicinal plants from Reunion Island for antimalarial and cytotoxic activity

Jonville, Marie-Caroline; Kodja, Hippolyte; Humeau, Laurence; Fournel, Jacques; Mol, Patrick De; Cao, Martine; Angenot, Luc; Frédérich, Michel

doi:10.1016/j.jep.2008.09.005

Cited by 122 publications

(88 citation statements)

References 16 publications

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“…According to WHO guidelines and previous results from our team (Jonville et al, 2008;Pink et al, 2005), antiplasmodial activity of extracts was classified as follows: highly active extracts with IC 50 < 5 µg/ml, promising activity at 5-15 µg/ml, moderate activity at 15-50 µg/ml and inactivity at > 50µg/ml. Among the five plants evaluated, three showed a significant antiplasmodial activity with an IC 50 < 15μg/ml for their dichloromethane and methanolic extracts against the two strains of Plasmodium falciparum (A. chinensis, P. angulata and S. icaja ), and two were highly active.…”

Section: Resultsmentioning

confidence: 99%

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In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

Lusakibanza

Mesia

Tona

et al. 2010

Journal of Ethnopharmacology

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Aim of the studyThe in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ethnobotanical survey conducted in D.R. Congo and focusing on plants used traditionally to treat malaria. The in vivo antimalarial activity of aqueous and methanolic extracts active in vitro was also determined in mice infected by Plasmodium berghei berghei. Materials and methodsThe growth inhibition of Plasmodium falciparum strains was evaluated using the measurement of lactate dehydrogenase activity. The extracts (aqueous, CH 3 OH, EtOH and CH 2 Cl 2 ) were prepared by maceration and tested in vitro against the 3D7 (chloroquine sensitive) and W2 (chloroquine resistant) strains of Plasmodium falciparum and against the human normal fetal lung fibroblasts WI-38 to determine the selectivity index. Some extracts were also used at the dose of 300mg/kg to evaluate their activity in mice infected since 4 days by Plasmodium berghei. ResultsTwo plants presented a very high activity (IC 50 < 3µg/ml). These plants were Strychnos icaja roots bark (MeOH and CH 2 Cl 2 ) and Physalis angulata leaves (MeOH and CH 2 Cl 2 ). One plant (Anisopappus chinensis whole plant, MeOH and CH 2 Cl 2 ) presented a high activity (IC50 < 15µg/ml).The extracts of Anisopappus chinensis and Physalis angulata showed also a good inhibition of parasitemia in vivo. Flavonoids, phenolic acids and terpenes were identified in these plants by a general phytochemical screening method. ConclusionThree plants showed a very interesting antiplasmodial activity (Anisopappus chinensis, Physalis angulata, Strychnos icaja) and one of them showed a good selectivity index ( >10, Anisopappus chinensis). Anisopappus chinensis and Physalis angulata were also active in vivo.

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Section: Resultsmentioning

confidence: 99%

“…hours of incubation at 37 ºC, the level of parasitaemia was estimated by measuring lactate dehydrogenase activity, as previously described (Jonville et al, 2008). The results were expressed as the mean IC 50 (the concentration of a drug that reduced the level of parasitaemia to 50%).…”

Section: In Vitro Antiplasmodial Assaysmentioning

confidence: 99%

In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

Lusakibanza

Mesia

Tona

et al. 2010

Journal of Ethnopharmacology

Self Cite

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“…According to Jonville et al [21] antiplasmodial activity has been classified as follows: high (CC 50 < 5 μg/ml), promising (5 < C-C 50 < 15 μg/ml), moderate (15 < CC 50 < 50 μg/ml) and inactive (CC 50 > 50 μg/ml). This is the first report showing antiplasmodial activity of W. frutescens.…”

Section: Discussionmentioning

confidence: 99%

In vitro antiplasmodial activity of Withania frutescens —Solanaceae

Bouzidi

Bakrim

Mahiou

et al. 2017

European Journal of Integrative Medicine

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A B S T R A C TIntroduction: Drugs resistant Plasmodium falciparum is a recurring issue that threatens public health. New anti-plasmodial drugs are needed to overcome this problem. The aim of this study was to characterize in vitro an-tiplasmodial activity of Withania frutescens (Solanaceae) against chloroquine-resistant Plasmodium falciparum strain. Methods: The in vitro antiplasmodial activity of leaves and roots extracts from Withania frutescens was performed in 96 well plates and preliminary phytochemical analysis was performed for the active fractions. The toxicity of the plant extract against CHO (Chinese Hamster Ovary) cells was assessed using the tetrazolium salt MTT col-orimetric assay. The selectivity index (SI) was calculated as the ratio between the cytotoxic and antiparasitic activities. Results: The methanol extract of W. frutescens leaves showed a good antiplasmodial activity (CC 50 18.1 μg/ml). Furthermore, ethyl acetate and butanol fractions showed promising in vitro antiplasmodial activity with a se-lectivity index of 3.1 and 2.4, respectively. The roots of W. frutescens were found to be inactive with a CC 50 value > 80 μg/ml. Conclusion: The antiplasmodial activity of W. frutescens may in part be attributed to the presence of polyphenolic and flavonoid compounds. Based on our results, ethyl acetate and butanol leaves fractions could be considered as a promising source for the development of putative antiplasmodial drugs.

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“…Extractos con CI • 50 anti-Plasmodium inferior a 50 µg/ml en las cepas NF54 o FCB2 de P. falciparum (17).…”

Section: Criterios De Selección De Extractos Promisoriosunclassified

Evaluación del efecto citotóxico y del daño genético de extractos estandarizados de Solanum nudum con actividad antiplasmodial

García-Huertas

Pabón

Arias³

et al. 2012

biomedica

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Contribución de los autores:Paola García diseñó la investigación, realizó las pruebas experimentales, tabuló los datos, analizó los resultados obtenidos y redactó el manuscrito. Adriana Pabón asesoró las pruebas de actividad anti-Plasmodium y análisis de los resultados, y participó en la corrección del manuscrito. Carlos Arias Londoño cuantificó las sustancias rastreadoras en los extractos estandarizados. Silvia Blair participó en el diseño de la investigación y en la supervisión del desarrollo de la misma; revisó y corrigió el artículo de investigación. ARTÍCULO ORIGINAL Biomédica 2013;33:78-87 doi: http://dx.doi.org/10.7705/biomedica.v33i1.838 Evaluación del efecto citotóxico y del daño genético de extractos estandarizados de Solanum nudum con actividad anti-Plasmodium Introducción. La planta Solanum nudum es ampliamente usada en la medicina tradicional del Pacífico colombiano para tratar las fiebres y la malaria, o paludismo, y se ha convertido en una fuente de nuevas moléculas promisorias. Objetivo. Evaluar el efecto citotóxico y daño genético de extractos estandarizados de S. nudum en diferentes modelos celulares. Materiales y métodos. A 66 extractos estandarizados de S. nudum se les evaluó la actividad antiPlasmodium in vitro en dos cepas de Plasmodium falciparum, una sensible (NF54) y otra resistente (FCB2) a la cloroquina, y la citotoxicidad en células U937 y HepG2. Se seleccionaron los extractos que presentaron actividad anti-Plasmodium y baja toxicidad, y se les estimó su efecto hemolítico en eritrocitos sanos O + , el efecto mutagénico en las cepas TA98 y TA100 de Salmonella Typhimurium y el efecto genotóxico en células U937. Resultados. Se seleccionaron cinco extractos como promisorios (28MA1, 29MA1, 51MA1, 55MA1 y 61MA1), los cuales fueron activos en las cepas de P. falciparum con concentración inhibitoria 50 (CI 50 ) entre 9,8 y 54,8 µg/ml. El extracto 29MA1 fue el más selectivo para Plasmodium, con índice de selectividad de 4,4 y 14,5 para las células U937 y HepG2, respectivamente. En ningún extracto se observó efecto hemolítico a 250 µg/ml, no causaron mutaciones en las cepas TA98 y TA100 de S. Typhimurium, ni generaron efectos genotóxicos en células U937. Conclusiones. La utilización de extractos estandarizados de S. nudum contribuye con los trabajos encaminados al desarrollo de una nueva formulación farmacéutica para tratar la malaria a partir de productos naturales.Palabras clave: Solanum/toxicidad, Plasmodium falciparum, malaria/terapia, extractos vegetales, seguridad. doi: http://dx.doi.org/10.7705/biomedica.v33i1.838Evaluation of cytotoxic effect and genetic damage of standardized extracts of Solanum nudum with antiplasmodial activity Introduction. The plant Solanum nudum (Solanaceae) is extensively used for the treatment of malariarelated symptoms in traditional medicine practices in the Colombian Pacific. Recently, it has become a significant source of promising new molecules for developing a pharmaceutical malaria treatment. Objective. This research aimed to evaluate the cytotoxic ...

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Screening of medicinal plants from Reunion Island for antimalarial and cytotoxic activity

Cited by 122 publications

References 16 publications

In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

In vitro antiplasmodial activity of Withania frutescens —Solanaceae

Evaluación del efecto citotóxico y del daño genético de extractos estandarizados de Solanum nudum con actividad antiplasmodial

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