Screening of Microsatellite Markers for Genetic Diversity Assessment and Conservation of Germplasm in Okra (Abelmoschus esculentus L. Moench)

Kumar, Mukesh; Sharma, Vaibhav; Kumar, Nirbhay; Sirohi, Ujjawal; Naresh, R.K.; Chaudhary, Veena

doi:10.20546/ijcmas.2017.606.060

Cited by 9 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…India is the largest okraplanted country, with an area of over 0.5 million ha, followed by Nigeria and Sudan, which produce 6.3 million MT per year (Varmudy 2011; Faostat 2014). Extensive previous research has mostly focused on cropping systems (Anonymous 2015), nutritional components (Calisir et al 2005), heredity and breeding (Kumar et al 2017;Mishra et al 2017), fresh maintenance (Tajiri 2011) and processing technology (Rai and Balasubramanian 2009). However, reports on the molecular mechanisms of okra are relatively few.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome sequencing of okra (Abelmoschus esculentus L. Moench) uncovers differently expressed genes responding to drought stress

Shi

Wang

Bai

et al. 2019

J. Plant Biochem. Biotechnol.

View full text Add to dashboard Cite

Drought affects the growth and development of plants and can cause crop failure. As an important edible and medicinal plant, okra (Abelmoschus esculentus L. Moench) is very popular in China and shows strong resistance to drought. In this study, gene expression profiles of okra leaves were investigated under water-limited conditions at the seedling stage. A total of 94,769 unigenes with an average length of 1921 bp and an N90 of 981 bp were obtained through next-generation sequencing. Furthermore, 38.18% of total unigenes were annotated in NT, NR, COG, KEGG, and SwissProt databases. A total of 2276, 5626, 11,719, 14,892, 855, 4593, and 5170 differentially expressed genes (DEGs) in seven pairwise comparisons (A5 vs. A0; A7 vs. A0; A15 vs. A0; A20 vs. A0; A7 vs. A5; A15 vs. A7, and A20 vs. A15) were identified. Among these, 22,751 DEGs were up-regulated, and the number was slightly more than that of the down-regulated DEGs. The number of up-regulated DEGs among different samples increased with increasing drought stress. We identified that the majority of DEGs were related to ''integral component of membrane'', ''oxidation-reduction process'', and ''metal ion binding'' categories. Pathway analysis revealed that most DEGs were enriched in ''Biosynthesis of secondary metabolites'', ''Carbon metabolism'', and ''Glycolysis/Gluconeogenesis pathways''. Additionally, a total of 17,456 simple sequence repeat motifs, 47,9831 single nucleotide polymorphisms, and 3746 transcription factors were identified in this study. RNA-Seq data were also verified by RT-qPCR. Our findings engender a further understanding of molecular mechanisms in response to drought stress. Keywords Okra (Abelmoschus esculentus L.) Á Transcriptome sequencing Á Drought stress Á Differential expression Abbreviations RNA-Seq Ribonucleic acid-sequencing NGS Next generation sequencing cDNA Complementary deoxyribonucleic acid PCR Polymerase chain reaction EB Ethambutol GO Gene ontology KEGG Kyoto encyclopedia of genes and genomes CDSs Coding sequences SSR Simple sequence repeat Jiyue Wang and Yu Bai: co-first author.

show abstract

Section: Introductionmentioning

confidence: 99%

Transcriptome sequencing of okra (Abelmoschus esculentus L. Moench) uncovers differently expressed genes responding to drought stress

Shi

Wang

Bai

et al. 2019

J. Plant Biochem. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Phenotypic descriptors, based on morphological and agricultural traits, were widely used in the characterization of okra gene banks (Reddy, Haribabu, Ganesh, & Chandrasekhar, 2012;Yonas, Garedew, & Debela, 2014;Asare, Asare-Bediako, Agyarko, Taah, & Osei, 2016;Ramgiry & Singh, 2017). However, although the genetic basis of okra is considered broad (Saifullah, Rabbani, & Garvey, 2010), the use of only phenotypic descriptors in studies of genetic diversity is restrictive, since phenotypes may be influenced by environmental factors or stages of plant development (Akash, Shiyab, & Saleh, 2013;Kumar et al, 2017b).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity among Brazilian okra landraces detected by morphoagronomic and molecular descriptors

et al. 2019

View full text Add to dashboard Cite

The conservation of okra landraces [Abelmoschus esculentus (L.) Moench] in gene banks is essential for the success of their use in breeding programmes. This study evaluated the genetic diversity among okra landraces in Brazil based on morphoagronomic descriptors and AFLP markers. We studied 30 accessions of the vegetable gene bank of the Universidade Federal de Viçosa. To this end, 17 morphoagronomic descriptors and five combinations of AFLP primers were used. Genetic parameters were estimated for the quantitative traits and the accessions were grouped by Ward’s method, using the Gower’s and Jaccard’s distance measures, respectively, for the morphoagronomic and molecular data. Polymorphisms were observed for all qualitative traits, while the quantitative traits were significant by deviance analysis. The genetic parameters confirmed the existence of variability among accessions, and high accuracy and heritability indices were found for the traits related to fruit and plant height. Ward’s grouping showed no relationship between the clusters formed with the morphoagronomic and molecular data and the geographical origin of the accessions. No association between morphoagronomic descriptors and AFLP markers was observed. The lack of correlation suggests that both approaches of characterization are important to understand and differentiate the okra accessions.

show abstract

“…Hence it is clear from the result that some okra germplasm used in this study belongs to a restricted germplasm pool. However, the accessions that were found to share common similarities further show a narrow genetic base as reported by Kumar et al (2017). High similarities among the accessions as reported by Hamon and Koechlin (1991) were expected due to their high selfpollinating properties (Hamon & Koechlin, 1991;Kumar et al, 2017).…”

Section: Discussionmentioning

confidence: 70%

“…However, the accessions that were found to share common similarities further show a narrow genetic base as reported by Kumar et al (2017). High similarities among the accessions as reported by Hamon and Koechlin (1991) were expected due to their high selfpollinating properties (Hamon & Koechlin, 1991;Kumar et al, 2017). Gulsen et al (2007) and Sharma et al (2015) also reported a similar result establishing a 100% similarity among grapefruit cultivars.…”

Section: Discussionmentioning

confidence: 70%

Morpho-Genetic Characterization of Abelmoschus Moench. Accessions

OBONGODOT¹,

Osawaru²,

Ogwu³

2022

Journal of Agricultural Production

View full text Add to dashboard Cite

This study was carried out to assess the morphological and genetic variability of seven accessions of Abelmoschus L. species using morphometric analysis and ribulose bisphosphate carboxylase large chain (RBCL) molecular markers. Using a completely randomized block design, seeds of the okra accessions were planted with three replicates each. During the developmental stage, the morphological features of the accessions were observed and recorded according to the standard descriptor for the crop. Morphologically, all the accessions exhibited a degree of similarities, albeit, at maturity, the leaf, plant height, leaf colour, and leaf shape became distinct. A cluster of the phenotypic characterization was observed at a 3.74 level of coefficient of similarity with two distinct clusters, which were predicted to be A. esculentus and A. caillei. The percentage variance of the two principal components was 55.12% and 22.69% with corresponding Eigenvalues of 4.11 and 1.69, respectively. Results of the RBCL analysis revealed genetic variability at a 0.80 level of coefficient of similarity. Two distinguishable clusters were observed. Both morphometric and genotyping results suggest that variations exist among and within the seven accessions. In conclusion, there is a need to frequently evaluate plant genetic resources held in gene banks as they may not reflect the whole range of diversity inherent in the species.

show abstract

Screening of Microsatellite Markers for Genetic Diversity Assessment and Conservation of Germplasm in Okra (Abelmoschus esculentus L. Moench)

Cited by 9 publications

References 10 publications

Transcriptome sequencing of okra (Abelmoschus esculentus L. Moench) uncovers differently expressed genes responding to drought stress

Transcriptome sequencing of okra (Abelmoschus esculentus L. Moench) uncovers differently expressed genes responding to drought stress

Genetic diversity among Brazilian okra landraces detected by morphoagronomic and molecular descriptors

Morpho-Genetic Characterization of Abelmoschus Moench. Accessions

Contact Info

Product

Resources

About