Screening of Multidrug Resistant Salmonella spp from Potable Water Collected from the Slum Areas of Bhopal, M.P. India

Ahirwar, Kamlesh Kumar; Tenguria, Rajesh Kumar

doi:10.20546/ijcmas.2020.902.051

Int.J.Curr.Microbiol.App.Sci

2020

DOI: 10.20546/ijcmas.2020.902.051

|View full text |Cite

Screening of Multidrug Resistant Salmonella spp from Potable Water Collected from the Slum Areas of Bhopal, M.P. India

Kamlesh Kumar Ahirwar¹,

Rajesh Kumar Tenguria²

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2022

Publication Types

Select...

Other1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Seas et al, children under 5 years of age, primarily in Asian and African countries, are seen as a gigantic gathering of patients contaminated with water-borne microbial infection 19 . Drinking water containing antibiotic-resistant bacterial strains is a serious concern according to scientific literature 20 . Due to resistant microorganisms' increasing pervasiveness, antibacterial therapies have become challenging to a large extent 21,22,23 .…”

mentioning

confidence: 99%

Untitled

2022

IJPSR

View full text Add to dashboard Cite

The incidence of multiple antibiotic-resistant Salmonella spp. isolates in randomly collected potable water samples from 70 different slum area sites of Bhopal City, M. P. India, were investigated through a molecular approach.BSA media was used for isolation and CFU count of Salmonella spp. The DNA of representative Salmonella spp. Colors of each water sample were subjected to PCR to detect Amp, cmlA and tetA genes with standard PCR protocol with annealing at primers specific temperature for 1 minute 30 seconds. The presence of the target gene in the PCR product was detected on 1% agarose gel. An in-vitro antibiogram study was done to check the phenotypic antibiotic resistance. A total 74.29% of samples were reported to have a prevalence of Salmonella spp. with 44.19average CFU count and huge standard deviation (σ=28.28). Incidence of Amp, cmlA, and tetA genes is reported to be 67.31%, 36.54%, and 61.54% in Salmonella spp. Isolates from 52 samples which doesn't coincide with the phenotypic prevalence of antibiotic resistance. 25, 17, and 11isolates showed resistance towards tetracycline, ampicillin, and chloramphenicol, respectively. The prevalence of phenotypic resistance towards ampicillin and incidence of amp gene in isolates were significant as the p < .05 in the case, contrarily chloramphenicol and tetracycline weren't significant for the incidence of their respective genes cmlA and tetA since their p-values are not significant at p < .05. However, the prevalence of phenotypic resistance towards chloramphenicol and tetracycline was independent of incidence of their respective genes in isolates is a matter of further extensive investigation on responsible factors thus increasing the residence time of drug with better patient compliance. INTRODUCTION:Water is the most studied hydrogen chalcogenide regarded as a "universal solvent which is transparent, colourless, tasteless, odourless, fluid and is one of the essential components on earth containing minerals important for all the organism on the surface of earth and QUICK RESPONSE CODE

show abstract

mentioning

confidence: 99%

Untitled

2022

IJPSR

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Screening of Multidrug Resistant Salmonella spp from Potable Water Collected from the Slum Areas of Bhopal, M.P. India

Cited by 1 publication

References 8 publications

Untitled

Untitled

Contact Info

Product

Resources

About