Screening of mycotoxins in animal feed from the region of Vojvodina

Abstract

This paper shows the results of screening of mycotoxins in animal feed originating from the region of Vojvodina. Permanent screening is needed on all levels of production and storage, as well as the use of known methods to reduce mould contamination or toxin content in feedstuffs and feed. A total of 56 representative samples were collected from feed companies from the region of Vojvodina. Samples were collected during February 2009. The collected samples included 41 samples of feedstuffs (soybean, soybean meal, soybean grits, soybean cake, maize, sunflower meal, barley, wheat feed flour, rapeseed meal, dehydrated sugar beet pulps, alfalfa meal, yeast, dried whey, fish meal, meat-bone meal) and 15 samples of complete feedingstuffs. The amounts of aflatoxins, ochratoxin A, zearalenone, fumonisin and deoxynivalenol were determined. Screening method for the analysis was done using Neogen Veratox® testing kits. The test itself is a competitive direct enzyme-linked immunosorbent assay (CD-ELISA). Mycotoxins were present in 71.4% of the samples, but the values determined were below the maximum allowed limits for both Serbian and EC reference values. Zearalenone was found with the highest incidence (57.1% of samples), followed by ochratoxin A (37.5%), fumonisin (33.9%), deoxynivalenol (14.3%) and aflatoxins (3.6%)

Aflatoxin M1 occurrence in Serbian milk and its impact on legislative

“…In 70 cow's milk samples, Polovinski-Horvatović et al (2010) used TLC method after immunoaffinity column clean-up to determine AFM1 and have found that none were contaminated with AFM1 in concentration greater than 0.05 µg/kg. Lower occurrence of AFM1 contamination reported in these reports from Serbia, compared to the results obtained in this and subsequent studies, can be explained with the absence of AFs in maize and other feed material in Serbia in previous years (Kokić et al, 2009;Jakšić et al, 2011;. In all the reports, ELISA test kit was used for AFs determination.…”

Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography

“…Typically, AFB 1 is not found in fishmeal because its matrix is unsuitable for the production of mycotoxin. It has been presented that AFB 1 was not detected in any fishmeal samples [ 32 ]. However, fishmeal contaminated with AFB 1 has been reported.…”

Risk assessment for mycotoxin contamination in fish feeds in Europe

“…Contamination of aquaculture feeds and naturally contaminated experimental fish feeds with the mycotoxins AFB 1 , ZEN, DON, FB 1 , T-2 and OTA reported in the literature (according to the studies ofBoonyaratpalin et al 2001;Kokic et al 2009;Deng et al 2010;Huang et al 2011;Rajeev Raghavan et al 2011;Pietsch et al 2013;Greco et al 2015) …”