Screening of natural Wolbachia infection in Aedes aegypti, Aedes taeniorhynchus and Culex quinquefasciatus from Guadeloupe (French West Indies)

Goindin, Daniella; Cannet, Arnaud; Delannay, Christelle; Ramdini, Cédric; Gustave, Joël; Atyame, Célestine M.; Vega-Rúa, Anubis

doi:10.1016/j.actatropica.2018.06.011

Cited by 21 publications

(14 citation statements)

References 24 publications

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“…The high Wolbachia infection in Cx. quinquefasiatus from Guadeloupe [35] has been also suspected to confer refractoriness to ZIKV, but experimental studies showed that even when Wolbachia is removed from this mosquito species, ZIKV fails to infect the midgut epithelial cells [20]. Taken together, these results show that Cx.…”

Section: Discussionmentioning

confidence: 98%

Transmission potential of African, Asian and American Zika virus strains by Aedes aegypti and Culex quinquefasciatus from Guadeloupe (French West Indies)

Héry

Boullis

Delannay

et al. 2019

Emerging Microbes & Infections

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Zika virus (ZIKV) is an arbovirus that has dramatically spread in South America and the Caribbean regions since 2015. The majority of vector incrimination studies available for ZIKV showed that Aedes aegypti mosquitoes are important vectors for this virus. However, several reports suggest that Culex quinquefasciatus mosquitoes may be implicated in ZIKV transmission in certain urban settings. In the present study, we evaluated the vector competence for ZIKV of Cx. quinquefasciatus and Ae. aegypti mosquitoes from Guadeloupe using African, American and Asian strains. The results demonstrated that Cx. quinquefasciatus is refractory to ZIKV infection whatever the strain tested at 7, 14 or 21 days post-infection (dpi), while ZIKV transmission was recorded in Ae. aegypti for all the three strains. The African ZIKV strain was better transmitted by Ae. aegypti (∼ 50% mean transmission efficiency) and with a shorter incubation period (7 dpi) when compared to the Asian and American strains (<14% transmission efficiency; incubation period of 14–21 dpi). Taken together, these results suggest that only Ae. aegypti mosquitoes are involved in urban ZIKV transmission in Guadeloupe and highlight a higher infectiousness of the African ZIKV strain in this mosquito species when compared to the Asian and American ones.

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Section: Discussionmentioning

confidence: 98%

Transmission potential of African, Asian and American Zika virus strains by Aedes aegypti and Culex quinquefasciatus from Guadeloupe (French West Indies)

Héry

Boullis

Delannay

et al. 2019

Emerging Microbes & Infections

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“…7a) confirms previous observations about the broad distribution of Cx . quinquefasciatus populations with Wolbachia as endosymbiotic bacteria in Guadeloupe [39]. This study from Goindin and colleagues reported a 95.8% positive rate of Wolbachia sp.…”

Section: Discussionmentioning

confidence: 99%

Stable distinct core eukaryotic viromes in different mosquito species from Guadeloupe, using single mosquito viral metagenomics

et al. 2019

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Background Mosquitoes are the most important invertebrate viral vectors in humans and harbor a high diversity of understudied viruses, which has been shown in many mosquito virome studies in recent years. These studies generally performed metagenomics sequencing on pools of mosquitoes, without assessment of the viral diversity in individual mosquitoes. To address this issue, we applied our optimized viral metagenomics protocol (NetoVIR) to compare the virome of single and pooled Aedes aegypti and Culex quinquefasciatus mosquitoes collected from different locations in Guadeloupe, in 2016 and 2017. Results The total read number and viral reads proportion of samples containing a single mosquito have no significant difference compared with those of pools containing five mosquitoes, which proved the feasibility of using single mosquito for viral metagenomics. A comparative analysis of the virome revealed a higher abundance and more diverse eukaryotic virome in Aedes aegypti , whereas Culex quinquefasciatus harbors a richer and more diverse phageome. The majority of the identified eukaryotic viruses were mosquito-species specific. We further characterized the genomes of 11 novel eukaryotic viruses. Furthermore, qRT-PCR analyses of the six most abundant eukaryotic viruses indicated that the majority of individual mosquitoes were infected by several of the selected viruses with viral genome copies per mosquito ranging from 267 to 1.01 × 10 8 (median 7.5 × 10 6 ) for Ae . aegypti and 192 to 8.69 × 10 6 (median 4.87 × 10 4 ) for Cx . quinquefasciatus . Additionally, in Cx . quinquefasciatus , a number of phage contigs co-occurred with several marker genes of Wolbachia sp. strain wPip. Conclusions We firstly demonstrate the feasibility to use single mosquito for viral metagenomics, which can provide much more precise virome profiles of mosquito populations. Interspecific comparisons show striking differences in abundance and diversity between the viromes of Ae . aegypti and Cx . quinquefasciatus . Those two mosquito species seem to have their own relatively stable "core eukaryotic virome", which might have important implications for the competence to transmit important medically relevant arboviruses. The presence of Wolbachia in Cx . quinquefasciatus might explain (1) the lower overall viral load compared to Ae . aegypti ...

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“…It is becoming more apparent that a variety of factors contribute to this variation, but we have a lack of understanding regarding why some taxa are present in a host, yet others are absent. In mosquitoes and other insects, much effort has been undertaken to characterize the infection status of species and populations for specific endosymbiotic bacteria such as Wolbachia [6][7][8][9], yet few studies have examined the infection prevalence of specific gut-associated bacteria in mosquito vectors. It is evident that several gut-associated bacterial taxa are common between phylogenetically diverse mosquito species [4,5], but less attention has been paid to identifying incompatible hos-microbe associations and the mechanism(s) behind this incompatibility.…”

Section: Introductionmentioning

confidence: 99%

Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity

et al. 2020

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How microbe–microbe interactions dictate microbial complexity in the mosquito gut is unclear. Previously we found that, Serratia, a gut symbiont that alters vector competence and is being considered for vector control, poorly colonized Aedes aegypti yet was abundant in Culex quinquefasciatus reared under identical conditions. To investigate the incompatibility between Serratia and Ae. aegypti, we characterized two distinct strains of Serratia marcescens from Cx. quinquefasciatus and examined their ability to infect Ae. aegypti. Both Serratia strains poorly infected Ae. aegypti, but when microbiome homeostasis was disrupted, the prevalence and titers of Serratia were similar to the infection in its native host. Examination of multiple genetically diverse Ae. aegypti lines found microbial interference to S. marcescens was commonplace, however, one line of Ae. aegypti was susceptible to infection. Microbiome analysis of resistant and susceptible lines indicated an inverse correlation between Enterobacteriaceae bacteria and Serratia, and experimental co-infections in a gnotobiotic system recapitulated the interference phenotype. Furthermore, we observed an effect on host behavior; Serratia exposure to Ae. aegypti disrupted their feeding behavior, and this phenotype was also reliant on interactions with their native microbiota. Our work highlights the complexity of host–microbe interactions and provides evidence that microbial interactions influence mosquito behavior.

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Screening of natural Wolbachia infection in Aedes aegypti, Aedes taeniorhynchus and Culex quinquefasciatus from Guadeloupe (French West Indies)

Cited by 21 publications

References 24 publications

Transmission potential of African, Asian and American Zika virus strains by Aedes aegypti and Culex quinquefasciatus from Guadeloupe (French West Indies)

Transmission potential of African, Asian and American Zika virus strains by Aedes aegypti and Culex quinquefasciatus from Guadeloupe (French West Indies)

Stable distinct core eukaryotic viromes in different mosquito species from Guadeloupe, using single mosquito viral metagenomics

Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity

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